Prevalence of amino acid mutations in hepatitis C virus core and NS5B regions among Venezuelan viral isolates and comparison with worldwide isolates
© Jaspe et al.; licensee BioMed Central Ltd. 2012
Received: 30 April 2012
Accepted: 18 September 2012
Published: 21 September 2012
Recent reports show that R70Q and L/C91M amino acid substitutions in the core from different hepatitis C virus (HCV) genotypes have been associated with variable responses to interferon (IFN) and ribavirin (RBV) therapy, as well to an increase of hepatocellular carcinoma (HCC) risk, liver steatosis and insulin resistance (IR). Mutations in NS5B have also been associated to IFN, RBV, nucleoside and non-nucleoside inhibitors drug resistance. The prevalence of these mutations was studied in HCV RNA samples from chronically HCV-infected drug-naïve patients.
After amplification of core and NS5B region by nested-PCR, 12 substitutions were analyzed in 266 Venezuelan HCV isolates subtype 1a, 1b, 2a, 2c, 2b, 2j (a subtype frequently found in Venezuela) and 3a (n = 127 and n = 228 for core and NS5B respectively), and compared to isolates from other countries (n = 355 and n = 646 for core and NS5B respectively).
R70Q and L/C91M core substitutions were present exclusively in HCV G1b. Both substitutions were more frequent in American isolates compared to Asian ones (69% versus 26%, p < 0.001 and 75% versus 45%, p < 0.001 respectively). In Venezuelan isolates NS5B D310N substitution was detected mainly in G3a (100%) and G1a (13%), this later with a significantly higher prevalence than in Brazilian isolates (p = 0.03). The NS5B mutations related to IFN/RBV treatment D244N was mainly found in G3a, and Q309R was present in all genotypes, except G2. Resistance to new NS5B inhibitors (C316N) was only detected in 18% of G1b, with a significantly lower prevalence than in Asian isolates, where this polymorphism was surprisingly frequent (p < 0.001).
Genotypical, geographical and regional differences were found in the prevalence of substitutions in HCV core and NS5B proteins. The substitutions found in the Venezuelan G2j type were similar to that found in G2a and G2c isolates. Our results suggest a high prevalence of the R70Q and L/C91M mutations of core protein for G1b and D310N substitution of NS5B protein for the G3a. C316N polymorphism related with resistance to new NS5B inhibitors was only found in G1b. Some of these mutations could be associated with a worse prognosis of the disease in HCV infected patients.
KeywordsHCV Core NS5B Mutation
Around 170 million persons (3% of the world population) are infected with the hepatitis C virus (HCV). More than 350,000 people die from HCV-related liver diseases, including hepatocellular carcinoma (HCC), each year . In Latin America around 7 million persons are infected with HCV and overall prevalence of HCV antibodies in non-Amerindian populations from South America is around 1% [2–4]. About 130 million people in the world are chronically infected with HCV and at risk of developing liver inflammation, fibrosis, cirrhosis and steatosis leading to HCC . Furthermore, in these chronic patients HCV infection has an increased risk of type-2 diabetes mellitus or insulin resistance (IR) .
Seven HCV genotypes, and a large number of subtypes in each genotype, have been described. Genotypes 1, 2, and 3 have a worldwide distribution, and their prevalence varies according to the region of the world. In Venezuela, genotype 1 is the most frequently detected, followed by genotypes 2 and 3, however a significant reduction of HCV G1b circulation was observed in the last decade, with the increase in circulation of genotype G2j, a subtype quite rare in other countries, including the Americas [6, 7].
While a vaccine for HCV is not available, many new antiviral agents are being tested to treat HCV infection, such as nucleoside (NIs) and non-nucleoside (NNIs) polymerase and protease inhibitors. The combination of pegylated interferon alpha (IFN) and ribavirin (RBV), plus new HCV NS3/4A serine protease inhibitors (boceprevir or telaprevir) is the current treatment for patients chronically infected with HCV . HCV genotype is also a predictive parameter for sustained virological response (SVR). Infections with HCV genotype 1 are associated with the lowest therapeutic success .
Within the HCV genome, core is thought to be the most conserved protein; results of nucleotide and deduced amino acid sequence analysis across diverse strains of HCV reveal 81–88% nucleotide and 96% amino acid sequence homology [10, 11]. The core gene region plays several essential roles in the HCV life cycle. Recent studies have revealed that amino acid substitutions in the core region of different HCV genotypes are associated with IR , increased HCC risk [13–17], variable responses to double (IFN/RBV) [18–24] and triple (telaprevir plus INF/RBV) therapy [25, 26], and liver steatosis [27–30]. On the other hand, the region of the viral genome that codes for NS5B has been described as an important target in therapy with IFN/RBV, and nucleoside and non-nucleoside drugs . Many drug-resistance mutations induced by antiviral treatment are located in this region of the genome [32–37].
In the present study, we analyzed core and NS5B polymorphisms/mutations which have been associated with non-responsiveness or a better response to IFN/RBV combination therapy, and/or resistance to polymerase inhibitor drugs, HCC, liver steatosis and IR, in chronically infected drug-naïve patients from Venezuela, and these polymorphisms/mutations were compared with worldwide HCV isolates.
Some relevant core and NS5B substitutions associated with treatment response, as well as, with a worse prognosis of the disease were analyzed. Four core substitutions [12–30] and eight NS5B substitutions [32–37] were studied in 266 Venezuelan HCV isolates from chronically infected, drug-naïve patients (n = 127 and n = 228 for core and NS5B respectively), and compared to isolates from other countries (n = 355 and n = 646 for core and NS5B respectively).
Frequency of amino acid substitution 70Q and 91 M of core protein in HCV Venezuelan isolates compared to worldwide isolates
p < 0.001
p < 0.001
Frequency of amino acid substitution in NS5B protein related to resistance to INF/RBV and new inhibitors in HCV Venezuelan isolates compared to worldwide isolates
Substitution (related to)
67/101 (66%) p = 0.004
11/82 (13%) p = 0.03
307/335 (91.6%) p < 0.001
Q309R and A333E NS5B mutations, frequents in patients with SVR and end-of-treatment response (ETR) after INF/RB therapy , were also analyzed. Q309R mutation was present in all genotypes (100% G3a, 44% G1a and 12% G1b from Venezuela), except G2 which harbored a Valine in this position (100% G2a, G2b, G2c and 91% G2j). This mutation was more frequently found in Brazilian G1a isolates compared to Venezuelan and USA isolates (Table 2). A333E mutation, only analyzed in Venezuelan G2 and G3 isolates, was present in most G2 samples (100% G2a, G2b, G2c and 98% G2j from Venezuela). G3a samples harbored Lysine or Arginine substitution at this position.
In addition, in the NS5B region, S282T and C316Y/N substitutions, associated to resistance to new NS5B inhibitors (2′-C-methyl modified ribonucleosides and HCV-796, respectively) [34–37], were also analyzed. S282T mutation (Table 2) and C316Y mutation were not found in any of the HCV isolates analyzed. However, C316N polymorphism was only detected in 18% of Venezuelan G1b isolates, in a similar prevalence to that found in other Latin American countries, but with a significantly lower frequency than those found in Japan (Table 2).
Others mutations that also confers resistance to others NNIs, have been identified downstream the NS5B region . These mutations could not be analyzed in this study, due to the length of the sequence analyzed.
A total of 12 amino acid positions were analyzed in core [12–30] and/or NS5B region [32–37] of Venezuelan HCV isolates and compared to worldwide isolates. Substitutions in these sites have been related either to IFN/RBV treatment or differential susceptibility to others drugs, and/or to a worse prognosis of the disease [12–30, 32–37]. These substitutions were found in 9/12 positions analyzed, with genotypical, geographical and/or regional differences in the prevalence of them.
R70Q and L/C91M core substitutions were found more frequently in Venezuelan and USA G1b isolates, compared to Asian ones. These amino acid substitutions in core region of different HCV genotypes are associated with increased HCC risk [13–17], variable responses to IFN/RBV therapy [18–24], liver steatosis [27–30] and IR . Treatment with IFN/RBV has been proposed to induce these amino acid mutations . Another possible explanation is that these mutations might be selected during the natural course of infection, in response to the selective pressure of endogenous IFN . The relatively high frequency of these mutations in patients not treated with IFN/RBV might be associated to the transmission of HCV isolates from treated patients. In addition, this relative high frequency in Latin America warrants further follow up studies in patients harboring one or two of these mutations. Steatosis motives in core domain 3 were exclusive to G3a, as previously reported .
Some mutations have been described in the NS5B region and appeared to be generated during IFN/RBV treatment, probably because of the mutagenic effect of RBV . D310N was detected in all G3a isolates. Interestingly, the frequency of this mutation was higher in Venezuelan G1a compared to Brazilian and USA isolates. Phylogenetic analysis of G1a isolates showed that the isolates carrying D310N were grouped in clades with high genetic relatedness, in agreement with the hypothesis of networks of transmission between non-treated patients infected with HCV isolates from a treated patient.
In addition, some of these mutations have been found more frequently in viruses infecting patients with sustained virological response (SVR) or during end-of-treatment response (ETR), in which the virus has been persistently or at least temporarily eliminated . From these, the Q309R and A333E mutations were analyzed in this study. In Venezuelan isolates Q309R was present in all genotypes except G2, very frequent in G3 and rare in G1b. In G1a the frequency of this mutation was similar in Venezuelan and USA isolates, but significantly lower than those found in Brazil. Instead, A333E was frequent in G2 and rare in G3, while it could not be analyzed in G1. These results are in agreement with the frequency found in other countries . The presence of these amino acid substitutions might be related to the relative susceptibility to IFN/RBV of G2 and G3 compared to G1.
S282T mutation in the NS5B polymerase is known to confer resistance to the inhibitor 2′-C-methyl modified ribonucleosides  with a decrease viral fitness . This mutation was not present in the isolates analyzed in this study.
For NNIs, resistance mutations may also occur as natural variants more frequently in patients infected with a specific subtype. For example, the C316Y mutation in NS5B is associated most frequently with resistance to HCV-796 . Furthermore, C316N also reduces sensitivity to HCV796 in vitro and this polymorphism is observed in many patients infected with HCV subtype 1b [41–43]. While C316Y mutation was not present in the isolates analyzed, C316N variant was detected in 18% of Venezuelan G1b isolates, with a significantly lower prevalence than in Asian isolates, where this mutation was found at high frequency (Table 2). Again, phylogenetic analysis showed that Venezuelan G1b isolates carrying the C316N were generally grouped in clades, displaying high genetic relatedness.
Two major clades have been described inside G1a isolates worldwide . Brazilian isolates belong mainly to one of these clades , and even form a cluster inside this clade . Venezuelan HCV isolates do not group together with Brazilian isolates (Figure 1). These results suggest that important regional variations might be found in HCV isolates circulating in South America. An example of this is the predominant circulation of G2j among the G2 isolates circulating in Venezuela, and not found in other neighboring countries .
Genotypical, geographical and regional differences were found in the prevalence of substitutions in HCV core and NS5B proteins. The substitutions found in the Venezuelan G2j type were similar to that found in G2a and G2c isolates. Our results suggest a high prevalence in Venezuela of the R70Q and L/C91M mutations of core protein for G1b and D310N substitution of NS5B protein for the G3a. As expected, C316N polymorphism, associated to resistance to NNIs was only found in G1b, and mutation S282T to NIs was absent. However, the presence of mutations associated to a worse prognosis of the disease in HCV-infected patients warrants further studies to analyze their impact in the clinical outcome of this disease in Venezuela.
Serum samples were collected from 1997 to 2010, from HCV-infected untreated patients after written informed consent, and stored at −30°C until use. This study was approved by the Bioethical Committee of Instituto Venezolano de Investigaciones Cientificas (IVIC). A total of 127 Venezuelan samples (27 infected with HCV-1a, 38 HCV-1b, 3 HCV-2b, 7 HCV-2c, 42 HCV-2j and 10 with HCV-3a) were processed for core amplification (57.5% male) and 228 (82 infected with HCV-1a, 61 HCV-1b, 1 with HCV-2a, 13 HCV-2b, 9 HCV-2c, 53 HCV-2j and 9 with HCV-3a) for NS5B amplification (58.3% male).
PCR and sequencing
HCV RNA was extracted from human plasma sample using a QIAamp® Viral Mini Kit (QIAGEN, Hilden Germany) and reverse-transcribed to complementary DNA using MMLV Reverse Transcriptase (Invitrogen life technologies, USA), according to the manufacturers protocol. HCV genotype was determined by direct sequencing and phylogenetic analysis of a polymerase chain reaction-amplified product from the 5' non-coding region , core and/or NS5B . The NS5B amplicons were generated previously . The core amplified PCR products were purified by using the QIAquick PCR Purification Kit (Qiagen) and then subjected to direct nucleotide sequencing. In all cases, both sense and antisense inner primers were used for sequencing and all sequences were performed by Macrogen Service Center, Seoul, Korea. A total of 127 core sequences were analyzed: 49 obtained previously  and 79 from this study.
Determination and analysis of core and NS5B amino acid sequence
Amino acid sequences were deduced and aligned using MEGA 4.0.2. The NS5B sequences [GenBank accession numbers HM777048-HM777357] and core sequences [GenBank accession numbers HM777360 -HM777430 and JQ924868-JQ924946] of Venezuelan HCV-infected naïve patients were analyzed. The sequences of each genotype from other countries were obtained from GenBank and HCV database (http:VHC.lanl.gov/content/VHC-db/index).
Sequence alignment performed by the global alignment algorithm, using DNAman 5.2.2 (Lynnon Bio Soft, Canada). Phylogenetic analysis was performed by the Neighbor Joining method (1000 bootstrap replicas, with genetic distances estimated with Kimura 2 parameters correction).
Statistical differences were evaluated by the Chi-Squares test with Yates correction, or Fisher Exact test, according to a computerized Epi Info program, version 3.5.3 (Centers for Disease Control and Prevention, Atlanta, GA).
Hepatitis C virus
Pegylated Interferon alpha
Non- nucleoside inhibitors
Sustained virological response.
This work was supported by Grant LOCTI from Venezuela.
- World health organization report June 2011, fact sheet N°164: http://www.who.int/mediacentre/factsheets/fs164/en/,
- Aguilar MS, Cosson C, Loureiro CL, Devesa M, Martínez J, Villegas L, Flores J, Ludert JE, Noya O, Liprandi F, Pujol FH, Alarcón de Noya B: Prevalence of hepatitis C virus infection in Venezuela assessed by a synthetic peptide-based immunoassay. Ann Trop Med Parasitol. 2001, 95: 187-195. 10.1080/00034980120042944.PubMedView ArticleGoogle Scholar
- Silva L, Paraná R, Mota E, Cotrim HP, Boënnec-McCurtey ML, Vitvitinsky L, Pádua A, Trepo C, Lyra L: Prevalence of hepatitis C virus in urban and rural populations of northeast brazil - pilot study. Arq Gastroenterol. 1995, 32: 168-171.PubMedGoogle Scholar
- Talarmin A, Kazanji M, Cardoso T, Pouliquen J-F, Sankale-Suzanon J, Sarthou JL: Prevalence of antibodies to hepatitis a, C, and E viruses in different groups in French Guiana. J Med Virol. 1997, 52: 430-435. 10.1002/(SICI)1096-9071(199708)52:4<430::AID-JMV15>3.0.CO;2-K.PubMedView ArticleGoogle Scholar
- Hung CH, Lee CM, Lu SN: Hepatitis C virus-associated insulin resistance: pathogenic mechanisms and clinical implications. Expert Rev Anti Infect Ther. 2011, 9 (5): 525-533. 10.1586/eri.11.33.PubMedView ArticleGoogle Scholar
- Pujol FH, Loureiro CL: Replacement of hepatitis C virus genotype 1b by genotype 2 over a 10 year period in Venezuela. J Clin Gastroenterol. 2007, 41: 518-520. 10.1097/01.mcg.0000248010.55149.ce.PubMedView ArticleGoogle Scholar
- Sulbarán MZ, Di Lello FA, Sulbarán Y, Cosson C, Loureiro CL, Rangel HR, Cantaloube JF, Campos RH, Moratorio G, Cristina J, Pujol FH: Genetic history of hepatitis C virus in Venezuela: high diversity and long time of evolution of HCV genotype 2. PLoS One. 2010, 5 (12): e14315-10.1371/journal.pone.0014315.PubMedPubMed CentralView ArticleGoogle Scholar
- Hofmann WP, Zeuzem S: A new standard of care for the treatment of chronic HCV infection. Nat Rev Gastroenterol Hepatol. 2011, 8 (5): 257-264.PubMedGoogle Scholar
- Fried MW, Hadziyannis SJ, Shiffman ML, Messinger D, Zeuzem S: Rapid virological response is the most important predictor of sustained virological response across genotypes in patients with chronic hepatitis C virus infection. J Hepatol. 2011, 55 (1): 69-75. 10.1016/j.jhep.2010.10.032.PubMedView ArticleGoogle Scholar
- Davis GL: Hepatitis C virus genotypes and quasispecies. Am J Med. 1999, 107: 21S-26S. 10.1016/S0002-9343(99)00376-9.PubMedView ArticleGoogle Scholar
- Simmonds P, Smith DB, McOmish F, Yap PL, Kolberg J, Urdea MS, Holmes EC: Identification of genotypes of hepatitis C virus by sequence comparisons in the core, E1 and NS-5 regions. J Gen Virol. 1994, 75 (Pt 5): 1053-1061.PubMedView ArticleGoogle Scholar
- Akuta N, Suzuki F, Hirakawa M, Kawamura Y, Yatsuji H, Sezaki H, Suzuki Y, Hosaka T, Kobayashi M, Kobayashi M, Saitoh S, Arase Y, Ikeda K, Kumada H: Amino acid substitutions in the hepatitis C virus core region of genotype 1b are the important predictor of severe insulin resistance in patients without cirrhosis and diabetes mellitus. J Med Virol. 2009, 81 (6): 1032-1039. 10.1002/jmv.21473.PubMedView ArticleGoogle Scholar
- Akuta N, Suzuki F, Seko Y, Kawamura Y, Sezaki H, Suzuki Y, Hosaka T, Kobayashi M, Hara T, Kobayashi M, Saitoh S, Arase Y, Ikeda K, Kumada H: The complicated relationships of amino acid substitution in HCV core region and IL28Bgenotype influencing hepatocarcinogenesis. Hepatology. In press
- Fishman SL, Factor SH, Balestrieri C, Fan X, Dibisceglie AM, Desai SM, Benson G, Branch AD: Mutations in the hepatitis C virus core gene are associated with advanced liver disease and hepatocellular carcinoma. Clin Cancer Res. 2009, 15 (9): 3205-3213. 10.1158/1078-0432.CCR-08-2418.PubMedPubMed CentralView ArticleGoogle Scholar
- Hu Z, Muroyama R, Kowatari N, Chang J, Omata M, Kato N: Characteristic mutations in hepatitis C virus core gene related to the occurrence of hepatocellular carcinoma. Cancer Sci. 2009, 100 (12): 2465-2468. 10.1111/j.1349-7006.2009.01338.x.PubMedView ArticleGoogle Scholar
- Kobayashi M, Akuta N, Suzuki F, Hosaka T, Sezaki H, Kobayashi M, Suzuki Y, Arase Y, Ikeda K, Watahiki S, Mineta R, Iwasaki S, Miyakawa Y, Kumada H: Influence of amino-acid polymorphism in the core protein on progression of liver disease in patients infected with hepatitis C virus genotype 1b. J Med Virol. 2010, 82 (1): 41-48. 10.1002/jmv.21629.PubMedView ArticleGoogle Scholar
- Nakamoto S, Imazeki F, Fukai K, Fujiwara K, Arai M, Kanda T, Yonemitsu Y, Yokosuka O, Nakamoto S, Imazeki F, Fukai K, Fujiwara K, Arai M, Kanda T, Yonemitsu Y, Yokosuka O: Association between mutations in the core region of hepatitis C virus genotype 1 and hepatocellular carcinoma development. J Hepatol. 2010, 52 (1): 72-78. 10.1016/j.jhep.2009.10.001. Erratum in: J Hepatol 2010, 52(4):620PubMedView ArticleGoogle Scholar
- Akuta N, Suzuki F, Hirakawa M, Kawamura Y, Sezaki H, Suzuki Y, Hosaka T, Kobayashi M, Kobayashi M, Saitoh S, Arase Y, Ikeda K, Chayama K, Nakamura Y, Kumada H: Amino acid substitution in HCV core/NS5A region and genetic variation near IL28B gene affect treatment efficacy to interferon plus ribavirin combination therapy. Intervirology. 2012, 55 (3): 231-241. 10.1159/000328327.PubMedView ArticleGoogle Scholar
- Akuta N, Suzuki F, Sezaki H, Suzuki Y, Hosaka T, Someya T, Kobayashi M, Saitoh S, Watahiki S, Sato J, Matsuda M, Kobayashi M, Arase Y, Ikeda K, Kumada H: Association of amino acid substitution pattern in core protein of hepatitis C virus genotype 1b high viral load and non-virological response to interferon-ribavirin combination therapy. Intervirology. 2005, 48 (6): 372-380. 10.1159/000086064.PubMedView ArticleGoogle Scholar
- Fukuhara T, Taketomi A, Okano S, Ikegami T, Soejima Y, Shirabe K, Maehara Y: Mutations in hepatitis C virus genotype 1b and the sensitivity of interferon-ribavirin therapy after liver transplantation. J Hepatol. 2010, 52 (5): 672-680. 10.1016/j.jhep.2009.10.034.PubMedView ArticleGoogle Scholar
- Inoue Y, Hiramatsu N, Oze T, Yakushijin T, Mochizuki K, Fukuda K, Mita E, Haruna Y, Inoue A, Imai Y, Hosui A, Miyagi T, Yoshida Y, Tatsumi T, Kiso S, Kanto T, Kasahara A, Takehara T, Hayashi N: Amino acid substitution in the core protein has no impact on relapse in hepatitis C genotype 1 patients treated with peginterferon and ribavirin. J Med Virol. 2011, 83 (3): 419-427. 10.1002/jmv.21975.PubMedView ArticleGoogle Scholar
- Kumthip K, Pantip C, Chusri P, Thongsawat S, O'Brien A, Nelson KE, Maneekarn N: Correlation between mutations in the core and NS5A genes of hepatitis C virus genotypes 1a, 1b, 3a, 3b, 6f and the response to pegylated interferon and ribavirin combination therapy. J Viral Hepat. 2011, 18 (4): e117-e125. 10.1111/j.1365-2893.2010.01379.x.PubMedView ArticleGoogle Scholar
- Kurbanov F, Tanaka Y, Matsuura K, Sugauchi F, Elkady A, Khan A, Hasegawa I, Ohno T, Tokuda H, Mizokami M: Positive selection of core 70Q variant genotype 1b hepatitis C virus strains induced by pegylated interferon and ribavirin. J Infect Dis. 2010, 201: 1663-1671. 10.1086/652500.PubMedView ArticleGoogle Scholar
- Akuta N, Suzuki F, Hirakawa M, Kawamura Y, Yatsuji H, Sezaki H, Suzuki Y, Hosaka T, Kobayashi M, Kobayashi M, Saitoh S, Arase Y, Ikeda K, Kumada H: Association of amino acid substitution pattern in core protein of hepatitis C virus genotype 2a high viral load and virological response to interferon-ribavirin combination therapy. Intervirology. 2009, 52 (6): 301-309. 10.1159/000235909.PubMedView ArticleGoogle Scholar
- Akuta N, Suzuki F, Hirakawa M, Kawamura Y, Yatsuji H, Sezaki H, Suzuki Y, Hosaka T, Kobayashi M, Kobayashi M, Saitoh S, Arase Y, Ikeda K, Kumada H: Amino acid substitutions in the hepatitis C virus core region of genotype 1b affect very early viral dynamics during treatment with telaprevir, peginterferon, and ribavirin. J Med Virol. 2010, 82 (4): 575-582. 10.1002/jmv.21741.PubMedView ArticleGoogle Scholar
- Akuta N, Suzuki F, Hirakawa M, Kawamura Y, Yatsuji H, Sezaki H, Suzuki Y, Hosaka T, Kobayashi M, Kobayashi M, Saitoh S, Arase Y, Ikeda K, Chayama K, Nakamura Y, Kumada H: Amino acid substitution in HCV core region and genetic variation near the IL28B gene affect viral dynamics during telaprevir, peginterferon and ribavirin treatment. Intervirology. 2012, 55 (6): 417-425. 10.1159/000323526.PubMedView ArticleGoogle Scholar
- Tachi Y, Katano Y, Honda T, Hayashi K, Ishigami M, Itoh A, Hirooka Y, Nakano I, Samejima Y, Goto H: Impact of amino acid substitutions in the hepatitis C virus genotype 1b core region on liver steatosis and hepatic oxidative stress in patients with chronic hepatitis C. Liver Int. 2010, 30 (4): 554-559. 10.1111/j.1478-3231.2009.02164.x.PubMedView ArticleGoogle Scholar
- Sumida Y, Kanemasa K, Hara T, Inada Y, Sakai K, Imai S, Yoshida N, Yasui K, Itoh Y, Okanoue T, Yoshikawa T: Impact of amino acid substitutions in hepatitis C virus genotype 1b core region on liver steatosis and glucose tolerance in non-cirrhotic patients without overt diabetes. J Gastroenterol Hepatol. 2011, 26 (5): 836-842. 10.1111/j.1440-1746.2010.06568.x.PubMedView ArticleGoogle Scholar
- Jhaveri R, McHutchison J, Patel K, Qiang G, Diehl AM: Specific polymorphisms in hepatitis C virus genotype 3 core protein associated with intracellular lipid accumulation. J Infect Dis. 2008, 197 (2): 283-291. 10.1086/524846.PubMedPubMed CentralView ArticleGoogle Scholar
- Jhaveri R, Qiang G, Diehl AM: Domain 3 of hepatitis C virus core protein is sufficient for intracellular lipid accumulation. J Infect Dis. 2009, 200 (11): 1781-1788. 10.1086/648094.PubMedPubMed CentralView ArticleGoogle Scholar
- Varshney J, Sharma PK, Sharma A: A review on an update of NS5B polymerase hepatitis C virus inhibitors. Eur Rev Med Pharmacol Sci. 2012, 16 (5): 667-671.PubMedGoogle Scholar
- Hamano K, Sakamoto N, Enomoto N, Izumi N, Asahina Y, Kurosaki M, Ueda E, Tanabe Y, Maekawa S, Itakura J, Watanabe H, Kakinuma S, Watanabe M: Mutations in the NS5B region of the hepatitis C virus genome correlate with clinical outcomes of interferon-alpha plus ribavirin combination therapy. J Gastroenterol Hepatol. 2005, 20 (9): 1401-1409. 10.1111/j.1440-1746.2005.04024.x.PubMedView ArticleGoogle Scholar
- Asahina Y, Izumi N, Enomoto N, Uchihara M, Kurosaki M, Onuki Y, Nishimura Y, Ueda K, Tsuchiya K, Nakanishi H, Kitamura T, Miyake S: Mutagenic effects of ribavirin and response to interferon/ribavirin combination therapy in chronic hepatitis C. J Hepatol. 2005, 43 (4): 623-629. 10.1016/j.jhep.2005.05.032.PubMedView ArticleGoogle Scholar
- Migliaccio G, Tomassini JE, Carroll SS, Tomei L, Altamura S, Bhat B, Bartholomew L, Bosserman MR, Ceccacci A, Colwell LF, Cortese R, De Francesco R, Eldrup AB, Getty KL, Hou XS, LaFemina RL, Ludmerer SW, MacCoss M, McMasters DR, Stahlhut MW, Olsen DB, Hazuda DJ, Flores OA: Characterization of resistance to non-obligate chain-terminating ribonucleoside analogs that inhibit hepatitis C virus replication in vitro. J Biol Chem. 2003, 278 (49): 49164-49170. 10.1074/jbc.M305041200.PubMedView ArticleGoogle Scholar
- Dutartre H, Bussetta C, Boretto J, Canard B: General catalytic deficiency of hepatitis C virus RNA polymerase with an S282T mutation and mutually exclusive resistance towards 2'-modified nucleotide analogues. Antimicrob Agents Chemother. 2006, 50 (12): 4161-4169. 10.1128/AAC.00433-06.PubMedPubMed CentralView ArticleGoogle Scholar
- Howe AY, Cheng H, Johann S, Mullen S, Chunduru SK, Young DC, Bard J, Chopra R, Krishnamurthy G, Mansour T, O'Connell J: Molecular mechanism of hepatitis C virus replicon variants with reduced susceptibility to a benzofuran inhibitor, HCV-796. Antimicrob Agents Chemother. 2008, 52 (9): 3327-3338. 10.1128/AAC.00238-08.PubMedPubMed CentralView ArticleGoogle Scholar
- McCown MF, Rajyaguru S, Kular S, Cammack N, Nájera I: GT-1a or GT-1b subtype-specific resistance profiles for hepatitis C virus inhibitors telaprevir and HCV-796. Antimicrob Agents Chemother. 2009, 53 (5): 2129-2132. 10.1128/AAC.01598-08.PubMedPubMed CentralView ArticleGoogle Scholar
- Margeridon-Thermet S, Shafer RW: Comparison of the mechanisms of drug resistance among HIV, hepatitis B, and hepatitis C. Viruses. 2010, 2 (12): 2696-2739. 10.3390/v2122696.PubMedPubMed CentralView ArticleGoogle Scholar
- Furui Y, Hoshi Y, Murata K, Ito K, Suzuki K, Uchida S, Satake M, Mizokami M, Tadokoro K: Prevalence of amino acid mutation in hepatitis C virus core region among Japanese volunteer blood donors. J Med Virol. 2011, 83 (11): 1924-1929. 10.1002/jmv.22216.PubMedView ArticleGoogle Scholar
- Castilho MC, Martins AN, Horbach IS, Perez Rde M, Figueiredo FA, Pinto Pde T, Nabuco LC, de Lima DB, Tanuri A, Porto LC, Ferreira Júnior Oda C: Association of hepatitis C virus NS5B variants with resistance to new antiviral drugs among untreated patients. Mem Inst Oswaldo Cruz. 2011, 106 (8): 968-975.PubMedView ArticleGoogle Scholar
- Hang JQ, Yang Y, Harris SF, Leveque V, Whittington HJ, Rajyaguru S, Ao-Ieong G, McCown MF, Wong A, Giannetti AM, Le Pogam S, Talamás F, Cammack N, Nájera I, Klumpp K: Slow binding inhibition and mechanism of resistance of non-nucleoside polymerase inhibitors of hepatitis C virus. J Biol Chem. 2009, 284 (23): 15517-15529. 10.1074/jbc.M808889200.PubMedPubMed CentralView ArticleGoogle Scholar
- Le Pogam S, Seshaadri A, Kosaka A, Chiu S, Kang H, Hu S, Rajyaguru S, Symons J, Cammack N, Nájera I: Existence of hepatitis C virus NS5B variants naturally resistant to non-nucleoside, but not to nucleoside, polymerase inhibitors among untreated patients. Antimicrob Chemother. 2008, 61 (6): 1205-1216. 10.1093/jac/dkn085.View ArticleGoogle Scholar
- Plaza Z, Soriano V, Gonzalez Mdel M, Di Lello FA, Macias J, Labarga P, Pineda JA, Poveda E: Impact of antiretroviral therapy on the variability of the HCV NS5B polymerase in HIV/HCV co-infected patients. J Antimicrob Chemother. 2011, 66 (12): 2838-2842. 10.1093/jac/dkr385.PubMedView ArticleGoogle Scholar
- Pickett BE, Striker R, Lefkowitz EJ: Evidence for separation of HCV subtype 1a into two distinct clades. J Viral Hepat. 2011, 18 (9): 608-618. 10.1111/j.1365-2893.2010.01342.x.PubMedPubMed CentralView ArticleGoogle Scholar
- Lampe E, Espirito-Santo MP, Martins RM, Bello G: Epidemic history of hepatitis C virus in brazil. Infect Genet Evol. 2010, 10 (7): 886-895. 10.1016/j.meegid.2010.05.010.PubMedView ArticleGoogle Scholar
- Peres-da-Silva A, Almeida AJ, Lampe E: Genetic diversity of NS3 protease from Brazilian HCV isolates and possible implications for therapy with direct-acting antiviral drugs. Mem Inst Oswaldo Cruz. 2012, 107 (2): 254-261.PubMedView ArticleGoogle Scholar
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