Identification of swine influenza A virus and Stenotrophomonas maltophilia co-infection in Chinese pigs
- Dongjun Hou†1,
- Yuhai Bi†1, 2,
- Honglei Sun1,
- Jun Yang1,
- Guanghua Fu1,
- Yipeng Sun1,
- Jinhua Liu1, 3 and
- Juan Pu1, 4Email author
© Hou et al.; licensee BioMed Central Ltd. 2012
Received: 3 November 2011
Accepted: 14 August 2012
Published: 22 August 2012
Influenza virus virulence can be exacerbated by bacterial co-infections. Swine influenza virus (SIV) infection together with some bacteria is found to enhance pathogenicity.
SIV-positive samples suspected of containing bacteria were used for bacterial isolation and identification. Antimicrobial susceptibility testing was performed by disc diffusion methods. To investigate the interaction of SIV and the bacteria in vitro, guinea pigs were used as mammalian hosts to determine the effect on viral susceptibility and transmissibility. Differences in viral titers between groups were compared using Student’s t-test.
During surveillance for SIV in China from 2006 to 2009, seven isolates (24.14%) of 29 influenza A viruses were co-isolated with Stenotrophomonas maltophilia from nasal and tracheal swab samples of pigs. Antimicrobial susceptibility testing showed that the bacteria possessed a high level of resistance towards clinically used antibiotics. To investigate the interaction between these two microorganisms in influencing viral susceptibility and transmission in humans, guinea pigs were used as an infection model. Animals were inoculated with SIV or S. maltophilia alone or co-infected with SIV and S. maltophilia. The results showed that although no transmission among guinea pigs was observed, virus–bacteria co-infections resulted in higher virus titers in nasal washes and trachea and a longer virus shedding period.
This is the first report of influenza virus co-infection with S. maltophilia in the Chinese swine population. Increased replication of virus by co-infection with multidrug resistant bacteria might increase the infection rate of SIV in humans. The control of S. maltophilia in clinics will contribute to reducing the spread of SIV in pigs and humans.
KeywordsSwine influenza virus S. maltophilia Co-infections Guinea pigs
Influenza A virus infection is a clinically and economically important pathogen causing respiratory disease in pigs worldwide. Swine influenza virus (SIV) subtypes H1N1, H3N2 and H1N2 can also cause zoonotic disease with flu-like symptoms in humans . The recent emergence of the pandemic H1N1 virus was potentially of swine origin and provides a reminder that infection of pigs with influenza A viruses pose important public health concerns .
Influenza virus infections are usually exacerbated by secondary bacterial infections, and are one of the major causes of severe influenza pneumonia in humans, possibly due to the synergistic effect of these microorganisms during respiratory tract invasion [3, 4]. It was reported that co-infection of SIV and bacteria such as Streptococcus pneumoniae, Haemophilus influenzae or Staphylococcus aureus leads to higher morbidity and mortality in mammals [5–7].
From our swine influenza surveillance work from 2006 to 2009, samples were inoculated into specific pathogen free (SPF) eggs for viral isolation . It was found that inoculated embryonated eggs of some SIV-positive isolates died during the isolation procedure. Visual inspection of these eggs showed that the allantoic fluid was turbid. From previous experience, the majority of SIVs do not cause egg death, as any bacteria in the swab are killed by the multiple antibiotics in the transport medium. Thus, we hypothesized that the collected samples contained bacteria that was not killed by antibiotics in the transport medium, and that these multidrug resistant bacteria survived by escaping antibiotic treatment, and might contribute to an increased chance of co-infection with influenza virus due to the enhancement of viral susceptibility and interspecies transmission. In light of reports of SIV infections in humans, especially in individuals that have had direct contact with pigs , this study aimed to identify co-infecting bacteria and investigate their interactions with SIV in viral replication and transmission using a guinea pig infection model.
Bacterial isolation and identification
Biochemical profile of the isolate with API 20 NE system
Reduction of nitrates
Indole production (tryptophane)
Assimilation (Potassium gluconate)
Assimilation (capric acid)
Hydrolysis (β-glucosidase) (esculin)
Assimilation (adipic acid)
Hydrolysis (protease) (gelatin)
Assimilation (trisodium citrate)
Assimilation (phenylacetic acid)
Susceptibility in guinea pigs
Guinea pigs have recently been shown to be an alternative mammalian model for the study of human infection with influenza A virus, especially for the study of viral transmissibility . Thus, we used guinea pigs to evaluate the susceptibility and transmissibility of SIV or SIV co-inoculated with S. maltophilia. Among the seven swine influenza isolates, A/Swine/Guangdong/7/06 (H3N2) belonged to a recent human-like lineage and possessed the closest genomic homology with human virus A/Moscow/10/99 . This virus was therefore selected for the animal experiments.
Virus, clinical signs, virus replication, transmission and seroconversion of guinea pigs
Inoculated guinea pigs
Contact guinea pigs
Peak mean nasal wash titer ± SD (day)**
Seroconversion (HI titer range)***
Virus detected in nasal wash****
Seroconversion (HI titer range)
Swine influenza virus (SIV)
5.50 ± 0.6 (2)
SIV + S. maltophilia
6.25 ± 0.4 (2)
Transmissibility in guinea pigs
Virus was not detected in any of the contact animals and seroconversion tests were all negative for SIV, suggesting that transmissibility did not occur in this experiment (Table 2).
Histopathology in guinea pigs
During the surveillance period (2006–2009), we found the first evidence for co-infection of SIV and S. maltophilia in pigs in several provinces in China, where the isolated S. maltophilia showed multidrug resistance. The co-infection of SIV and S. maltophilia has only previously been reported in Brazil .
S. maltophilia is an aerobic, non-fermentative, Gram-negative bacterium belonging to the genus Stenotrophomonas. S. maltophilia is ubiquitous in aqueous environments, soil and plants, and frequently colonizes breathing tubes such as endotracheal or tracheostomy tubes, the respiratory tract and indwelling urinary catheters. It is a causal agent of infection and has gained considerable prominence in recent years as an important nosocomial pathogen associated with significant case/fatality ratios in debilitated or immunosuppressed patients [13–17].
In 2004, S. maltophilia was first reported in pigs from China , which was followed by several reports describing bacteria infecting both healthy and diseased pigs [19–21]. However, the colonization rate of swine with S. maltophilia in China was unknown, and co-infection of S. maltophilia and influenza A virus had not been previously reported in the Chinese swine population.
In this study, the synergistic effect of SIV and S. maltophilia caused depression in co-infected guinea pigs. Higher virus titers in nasal washes and tracheal samples and a longer period of virus shedding in nasal washes of co-infected animals was found in comparison with animals infected only with either virus or bacteria. In addition, the co-infected group showed more severe pathological lesions in the trachea than the other groups. This in vivo finding suggests that co-infection can enhance SIV replication during infection of guinea pigs. However, replication of the virus was still confined to the upper respiratory tract and was not found in the lungs, as is the case for most human influenza viruses [22–24]. Swine influenza viruses can cause disease in humans . Several cases of co-infection of influenza A virus and S. maltophilia in humans have also been reported [11, 25]. S. maltophilia is a multidrug resistant bacterium and possesses a high degree of resistance towards most commonly used broad-spectrum antibiotics, which is also confirmed in this study [26, 27]. S. maltophilia can survive antibiotic treatment and co-infect hosts together with SIV, which can enhance viral replication and may increase the incidence of human infection with SIV, or SIV and S. maltophilia. During the treatment of SIV co-infection, controlling S. maltophilia will contribute to a reduction in illness and viral spread in pigs and human.
Here, we present data providing the first evidence for co-infection of influenza A virus and S. maltophilia in Chinese pigs. We show that co-infection contributed to the virulence of SIV in experimental guinea pigs. SIV and S. maltophilia pose an important public health concern, we believe it is of interest to further investigate co-infections involving these two infectious agents.
Material and methods
From 2006 to 2009, 3,546 nasal and tracheal swab samples were collected for influenza surveillance in four main swine industrial provinces in China (Beijing, Fujian, Guangdong and Shandong). Twenty-nine strains of swine influenza A virus, including 19 H1N1, a single H1N2 and 9 H3N2 strains were obtained. Genetic characterization of these viruses was reported in a previous study . Swab sample transport medium contained minimum essential medium (MEM), 2 × 107 IU/L penicillin G, 1 × 107 IU/L streptomycin, 100 mg/L gentamicin, 100 mg/L nystatin, 100 mg/L polymyxin B and 1000 mg/L sulfanilamide. SIV-positive samples suspected of containing bacteria (assessed by egg death and turbidity of the allantoic fluid) were used for bacterial isolation and identification.
Bacterial isolation and identification
SIV-positive swab samples suspected of containing bacteria were streaked onto LB agar plates and incubated at 37°C for 24 h. Bacterial colonies were collected and identified using a RiboPrinter Microbial Characterization System (DuPont Qualicon, DE, USA) . Biochemical characterization was performed using an API 20 NE (BioMerieux, France) and analyzed with Apiweb software (BioMerieux, France) according the manufacturer’s instructions . To confirm the identified result, several colonies selected randomly were used as template for 16S rRNA sequencing as previously described . Sequences were analyzed by Basic Local Alignment Search Tool (BLAST) for species identification as previously described .
Antimicrobial susceptibility testing
Antimicrobial susceptibility testing was performed by disc diffusion methods recommended by the Clinical and Laboratory Standards Institute (CLSI). The antimicrobial discs tested included ofloxacin, levofloxacin, streptomycin, sulfadimidine, gentamicin, azithromycin, trimethoprim, ciprofloxacin, minocycline, ampicillin, amoxicillin, novobiocin, vancomycin, nitrofurantoin, cefotaxime, ceftazidime. (supplied by Tiantan Company of Pharmaceutical and Biological Products Development, Beijing, China). Determination of the antimicrobial susceptibility test was performed according to the manufacturer’s instructions, which followed the criteria of the CLSI. Reference strains Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853 were used as quality control organisms in all antimicrobial susceptibility tests.
To investigate the interaction of SIV and S. maltophilia in influencing viral susceptibility and transmissibility in mammalian hosts, SPF/VAF (virus antibody free) Hartley strain female guinea pigs weighing 300–350 g and serologically negative for influenza virus were used. Animal experiments were approved by the Beijing Association for Science and Technology, the approve ID is SYXK (Beijing) 2007–0023, and complied with the guidelines of Beijing laboratory animal welfare and ethical of Beijing Administration Committee of Laboratory Animals. Zoletil 100 (tiletamine-zolazepam; Virbac S.A., Garros, France) was used to anesthetize animals by intramuscular injection (10–15 mg/kg).
Susceptibility in guinea pigs
Animals were inoculated with SIV or S. maltophilia alone or by co-infection of SIV and S. maltophilia. Groups of 18 animals were anesthetized and inoculated intranasally with 100 μL (106 50% egg infection dose, EID50) of virus, or 100 μL of 1.5 × 107 colony forming units (CFU) S. maltophilia, or a 100 μL mixture of the virus (106 EID50) and bacteria (1.5 × 107 CFU). Animals inoculated with Dulbecco’s phosphate-buffered saline (DPBS) were used as controls. On days 1, 2, 4, 6 and 8 p.i., three animals from each group were euthanized. Nasal washes, tracheas and lungs were collected and a part of each organ was homogenized in DPBS-A (DPBS containing 2,000 U/mL penicillin G and 2.5 mg/mL streptomycin) and then 0.10 mg/mL vancomycin was added (the S. maltophilia isolate was sensitive to vancomycin) for virus titration by EID50 assay. Nasal washes were performed by instilling a total of 1 mL of DPBS-A into the nostrils and collecting liquid runoff into a sterile Petri dish. To confirm successful bacterial inoculation, tracheas and lungs were also collected for isolation and identification on day 4 p.i., as previously described . Groups of the remaining three animals were observed for 2 weeks for body weight, signs of disease, and tested for SIV seroconversion on day 14 p.i.
Transmissibility in guinea pigs
For the contact transmission study, groups of 15 naive guinea pigs were housed with the virus-inoculated, or virus and bacteria co-inoculated animals at 24 h p.i. and observed for 21 days. On days 2, 4, 6 and 8 p.i., the nasal washes, tracheas and lungs of three contact animals for each group were collected and titrated for EID50 assay. Groups of the remaining three animals were observed over 21 days for body weight and signs of disease, and tested for seroconversion on day 21 post-contact.
On day 4 p.i., trachea and lung specimens from inoculated guinea pigs were fixed in 10% neutral buffered formalin, routinely processed, and embedded in paraffin. Sections (4-μm thickness) were stained with hematoxylin and eosin (HE).
Differences in viral titers between single virus-inoculated and virus-bacterial co-inoculated groups were compared using Student’s t-test. A P-value less than 0.05 was considered statistically significant.
The study was supported by the Specialized Research Fund for the Doctoral Program of Higher Education (20090008120007), National Science Fund for Distinguished Young Scholars (31025029), the National Basic Research Program (973 Program, 2011CB504702), and the Program for Cheung Kong Scholars and Chinese Universities Scientific Fund (Project No.2011JS013).
- Peiris JS, Poon LL, Guan Y: Emergence of a novel swine-origin influenza A virus (S-OIV) H1N1 virus in humans. J Clin Virol. 2009, 45: 169-173. 10.1016/j.jcv.2009.06.006.PubMedView Article
- Neumann G, Noda T, Kawaoka Y: Emergence and pandemic potential of swine-origin H1N1 influenza virus. Nature. 2009, 459: 931-939. 10.1038/nature08157.PubMedPubMed CentralView Article
- Callan RJ, Hartmann FA, West SE, Hinshaw VS: Cleavage of influenza A virus H1 hemagglutinin by swine respiratory bacterial proteases. J Virol. 1997, 71: 7579-7585.PubMedPubMed Central
- Scheiblauer H, Reinacher M, Tashiro M, Rott R: Interactions between bacteria and influenza A virus in the development of influenza pneumonia. J Infect Dis. 1992, 166: 783-791. 10.1093/infdis/166.4.783.PubMedView Article
- Cunha BA, Pherez FM, Durie N: Swine influenza (H1N1) and acute appendicitis. Heart Lung. 2010, 39: 544-546. 10.1016/j.hrtlng.2010.04.004.PubMedView Article
- Barlow GD: Swine flu and antibiotics. J Antimicrob Chemother. 2009, 64: 889-894. 10.1093/jac/dkp313.PubMedView Article
- Cunha BA, Syed U, Mickail N: Fulminant fatal swine influenza (H1N1): Myocarditis, myocardial infarction, or severe influenza pneumonia?. Heart Lung. 2010, 39: 453-458. 10.1016/j.hrtlng.2010.04.003.PubMedView Article
- Bi Y, Fu G, Chen J, Peng J, Sun Y, Wang J, Pu J, Zhang Y, Gao H, Ma G, et al: Novel swine influenza virus reassortants in pigs, China. Emerg Infect Dis. 2010, 16: 1162-1164. 10.3201/eid1607.091881.PubMedPubMed CentralView Article
- Nowotny N, Deutz A, Fuchs K, Schuller W, Hinterdorfer F, Auer H, Aspock H: Prevalence of swine influenza and other viral, bacterial, and parasitic zoonoses in veterinarians. J Infect Dis. 1997, 176: 1414-1415. 10.1086/517337.PubMedView Article
- Lowen AC, Mubareka S, Tumpey TM, Garcia-Sastre A, Palese P: The guinea pig as a transmission model for human influenza viruses. Proc Natl Acad Sci U S A. 2006, 103: 9988-9992. 10.1073/pnas.0604157103.PubMedPubMed CentralView Article
- Mancini DA, Mendonca RM, Dias AL, Mendonca RZ, Pinto JR: Co-infection between influenza virus and flagellated bacteria. Rev Inst Med Trop Sao Paulo. 2005, 47: 275-280.PubMedView Article
- Palleroni NJ, Bradbury JF: Stenotrophomonas, a new bacterial genus for Xanthomonas maltophilia (Hugh 1980) Swings et al. 1983. Int J Syst Bacteriol. 1993, 43: 606-609. 10.1099/00207713-43-3-606.PubMedView Article
- Roilides E, Butler KM, Husson RN, Mueller BU, Lewis LL, Pizzo PA: Pseudomonas infections in children with human immunodeficiency virus infection. Pediatr Infect Dis J. 1992, 11: 547-553. 10.1097/00006454-199207000-00008.PubMedView Article
- Denton M, Kerr KG: Microbiological and clinical aspects of infection associated with Stenotrophomonas maltophilia. Clin Microbiol Rev. 1998, 11: 57-80.PubMedPubMed Central
- Penland RL, Wilhelmus KR: Stenotrophomonas maltophilia ocular infections. Arch Ophthalmol. 1996, 114: 433-436. 10.1001/archopht.1996.01100130429013.PubMedView Article
- Vartivarian SE, Papadakis KA, Anaissie EJ: Stenotrophomonas (Xanthomonas) maltophilia urinary tract infection. A disease that is usually severe and complicated. Arch Intern Med. 1996, 156: 433-435. 10.1001/archinte.1996.00440040111012.PubMedView Article
- Waters VJ, Gomez MI, Soong G, Amin S, Ernst RK, Prince A: Immunostimulatory properties of the emerging pathogen Stenotrophomonas maltophilia. Infect Immun. 2007, 75: 1698-1703. 10.1128/IAI.01469-06.PubMedPubMed CentralView Article
- Zhang H, Xie M, Zhang J, Tan Z, Cai J, Wanjun G: Cloning and sequencing of 1 6 S rRNA gene of Stenotrophomonas maltophilia from swine. Chinese Journal of Veterinary Science and Technology. 2004, 34: 3-5.
- Zhengqin G, Qiang Z, Zhengming H, Bingfei Y, Qiang Y: Identification and susceptibility test of bacteria isolated from miniature pigs in China. Chinese Journal of Zoonoses. 2010, 26: 46-52.
- Long C, Wenyi X, Lixing W, Mi L, Qigui Y, Manli H, Yingchun F: Isolation, identification and pathogenicity of stenotrophomonas maltophilia strain derived from swine high fever cases. Progress in Veterinary Medicine. 2009, 30: 25-28.
- Xiangjie L, Sheng Y, Qiuquan W, Qingjun Z, Weiguo Z, Minxiang L, Sheng Z, Haojie Z: Study on the drug resistance and identification of Stenotrophomonas maltophilia isolated from swine. Fuoshan Science and Technology Institute Journal. 2008, 26: 41-43.
- Smith AM, Ribeiro RM: Modeling the viral dynamics of influenza A virus infection. Crit Rev Immunol. 2010, 30: 291-298. 10.1615/CritRevImmunol.v30.i3.60.PubMedView Article
- Bender BS, Small PJ: Influenza: pathogenesis and host defense. Semin Respir Infect. 1992, 7: 38-45.PubMed
- van Riel D, den Bakker MA, Leijten LM, Chutinimitkul S, Munster VJ, de Wit E, Rimmelzwaan GF, Fouchier RA, Osterhaus AD, Kuiken T: Seasonal and pandemic human influenza viruses attach better to human upper respiratory tract epithelium than avian influenza viruses. Am J Pathol. 2010, 176: 1614-1618. 10.2353/ajpath.2010.090949.PubMedPubMed CentralView Article
- Mancini DA, Alves RC, Mendonca RM, Bellei NJ, Carraro E, Machado AM, Pinto JR, Mancini FJ: Influenza virus and proteolytic bacteria co-infection in respiratory tract from individuals presenting respiratory manifestations. Rev Inst Med Trop Sao Paulo. 2008, 50: 41-46. 10.1590/S0036-46652008000100009.PubMedView Article
- Lecso-Bornet M, Bergogne-Berezin E: Susceptibility of 100 strains of Stenotrophomonas maltophilia to three beta-lactams and five beta-lactam-beta-lactamase inhibitor combinations. J Antimicrob Chemother. 1997, 40: 717-720. 10.1093/jac/40.5.717.PubMedView Article
- Sanders CC, Sanders WJ: beta-Lactam resistance in gram-negative bacteria: global trends and clinical impact. Clin Infect Dis. 1992, 15: 824-839. 10.1093/clind/15.5.824.PubMedView Article
- Pangloli P, Dje Y, Ahmed O, Doane CA, Oliver SP, Draughon FA: Seasonal incidence and molecular characterization of salmonella from dairy cows, calves, and farm environment. Foodborne Pathog Dis. 2008, 5: 87-96. 10.1089/fpd.2007.0048.PubMedView Article
- Bosshard PP, Zbinden R, Abels S, Boddinghaus B, Altwegg M, Bottger EC: 16S rRNA Gene Sequencing versus the API 20 NE System and the VITEK 2 ID-GNB Card for Identification of Nonfermenting Gram-Negative Bacteria in the Clinical Laboratory. J Clinical Microbiology. 2006, 44 (4): 1359-1366. 10.1128/JCM.44.4.1359-1366.2006.View Article
- Kajikazawa T, Sugita T, Nishikawa A: Comprehensive identification of bacteria in processed fresh edible sea urchin using 16S ribosomal DNA sequence analysis: The products contain various food poisoning-related bacteria and opportunistic bacterial pathogens. J Health Science. 2007, 53 (6): 756-759. 10.1248/jhs.53.756.View Article
- Tang HB, DiMango E, Bryan R, Gambello M, Iglewski BH, Goldberg JB, Prince A: Contribution of specific Pseudomonas aeruginosa virulence factors to pathogenesis of pneumonia in a neonatal mouse model of infection. Infect Immun. 1996, 64: 37-43.PubMedPubMed Central
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