Disassociation of the SV40 Genome from Capsid Proteins Prior to Nuclear Entry
© Kuksin and Norkin; licensee BioMed Central Ltd. 2012
Received: 16 December 2011
Accepted: 9 July 2012
Published: 10 August 2012
Previously, we demonstrated that input SV40 particles undergo a partial disassembly in the endoplasmic reticulum, which exposes internal capsid proteins VP2 and VP3 to immunostaining. Then, in the cytoplasm, disassembly progresses further to also make the genomic DNA accessible to immune detection, as well as to detection by an ethynyl-2-deoxyuridine (EdU)-based chemical reaction. The cytoplasmic partially disassembled SV40 particles retain some of the SV40 capsid proteins, VP1, VP2, and VP3, in addition to the viral genome.
In the current study, we asked where in the cell the SV40 genome might disassociate from capsid components. We observed partially disassembled input SV40 particles around the nucleus and, beginning at 12 hours post-infection, 5-Bromo-2-deoxyuridine (BrdU)-labeled parental SV40 DNA in the nucleus, as detected using anti-BrdU antibodies. However, among the more than 1500 cells examined, we never detected input VP2/VP3 in the nucleus. Upon translocation of the BrdU-labeled SV40 genomes into nuclei, they were transcribed and, thus, are representative of productive infection.
Our findings imply that the SV40 genome disassociates from the capsid proteins before or at the point of entry into the nucleus, and then enters the nucleus devoid of VP2/3.
KeywordsSV40 Polyomavirus Nuclear entry
Simian virus 40, as well as several other polyomaviruses (e.g. murine polyomavirus and BKV), are taken up into cells by virus-induced, caveola-mediated endocytosis [1–4]. The entry pathway of these viruses then follows a rather unusual route to the nucleus, by first passing through the endoplasmic reticulum (ER) [3, 5].
Previously, we demonstrated that SV40 particles undergo partial disassembly in the ER, as shown by the finding that within that organelle the internal capsid proteins, VP2 and VP3, become accessible to immunostaining with antibodies . More recently, we asked whether SV40 disassembly in the ER occurs to an extent that might also make the viral genome accessible to an antibody-based detection procedure. We found that the genomic DNA becomes accessible to each of two independent detection procedures, one based on detecting BrdU-labeled DNA with anti-BrdU antibodies and the other based on an EdU (ethynyl-2-deoxyuridine)-based chemical reaction, only after the partially disassembled SV40 particles emerge in the cytoplasm . The cytoplasmic partially disassembled SV40 particles retain at least some of each of the three SV40 capsid proteins, as well as the viral genome. Thus, SV40 particles undergo discrete disassembly steps during entry that are separated temporally and topologically. First, a partial disassembly of the particles occurs in the ER, which causes internal capsid proteins VP2 and VP3 to become accessible to detection with antibodies. Then, in the cytoplasm, disassembly progresses further to also make the genomic DNA accessible to immune detection, as well as to an EdU-based procedure.
A key unanswered question regarding the unique SV40 entry pathway concerns the location where the viral genome might be completely released from the capsid components. Experimental results reported here imply that the SV40 genome disassociates from the internal capsid proteins VP2 and VP3 prior to, or at the point of nuclear entry, and then enters the nucleus without them.
We incorporated BrdU into parental SV40 genomes, and used anti-BrdU antibodies to detect exposure of the BrdU-labeled DNA within partially disassembled viral particles, as well as to detect released SV40 genomes, as previously described . Exposed internal SV40 capsid proteins, VP2 and VP3, were detected using an antibody that recognizes a common epitope on these proteins . Because we can not distinguish between VP2 and VP3 using this antibody, we use the designation “VP2/3” to refer to the two proteins it recognizes. Note that under our conditions of incorporating BrdU into viral genomes (i.e., at 1 μg/ml), there was no effect on the kinetics of SV40 entry . Nor was there an effect on subsequent infectivity, as measured by expression of the SV40 large T antigen (LT, an early SV40 gene product) [7, 8].
Importantly, no VP2/3 can be seen within the nuclei of SV40-infected cells at either 8 or 12 hours post infection (Figures 1, 3). What is more, the fact that the cytoplasmic VP2/3 stain was considerably more intense than the nuclear BrdU DNA stain supports the premise that VP2/3 of parental virus particles does not enter the nucleus with the viral genome but, instead, remains behind in the cytoplasm.
Note that the intensity of the VP2/3 stain of the cytoplasmic VP2/3+BrdU- entities is less than that of the VP2/3+BrdU+ entities. This might be due to some VP2/3 loss from these particles when they release their DNA for entry into the nucleus; a finding consistent with the likelihood that the viral DNA plays a role in maintaining the structural integrity of the disassembly intermediates .
It may be possible that a small number of VP2/3 molecules, which is below our level of detection, do accompany the viral genome into the nucleus. Nevertheless, we can not ignore the fact that among the more than 1500 cells examined, we never detected input VP2/VP3 in the nucleus. Thus, our experimental findings imply that the SV40 genome separates from VP2/3 preceding or at the point of nuclear entry and thus enter the nucleus devoid of these viral proteins.
There is substantial evidence that nuclear localization signals (NLSs) on VP2/3 might mediate the targeting SV40 disassembly intermediates to the nucleus, and perhaps mediate nuclear entry as well. For example, microinjecting anti-VP2/3 antibodies into the cytoplasm blocks SV40 infection, and SV40 virus-like particles (VLPs), which contain NLS-deficient VP3, enter cells normally, but do not transport their DNA into the nucleus [10, 11]. In view of these findings, our initial working hypothesis was that exposed VP2/3 on the SV40 disassembly intermediates would target these particles to the nucleus, and also mediate the nuclear entry of the SV40 genomes. However, considering our current findings, we believe that while VP2/3 may target the SV40 disassembly intermediates to nuclear pore complexes (NPCs), SV40 capsid proteins do not mediate nuclear entry of the genome per se. The larger adenoviruses provide a precedent for such a state of affairs. Adenovirus, subviral capsids attach to the NPC via the hexon protein. Next, they recruit a cellular motor, kinesin-1, which facilitates both the uncoating of the viral genome and its entry into the nucleus, with capsid fragments being left behind in the cytoplasm .
Given our findings, we might ask the following. First, what factors might separate VP2/3 from the viral genome prior to nuclear entry? We can only speculate. Nevertheless, one possibility is that this stage of SV40 disassembly is facilitated by a cytoplasmic chaperone, such as cytoplasmic Hsp70. In this regard, Hsp70 binds to SV40 and disrupts murine polyomavirus in vitro [13, 14]. Moreover, the premise, that a cytoplasmic chaperone might facilitate the separation of VP2/3 from the viral genome, may account for at least some of the BrdU immunostain seen outside the nucleus that does not colocalize with VP2/3. The relative diffuseness of this stain, in comparison to the particle-associated BrdU stain, is consistent with this notion. It is possible that some of these “naked” cytoplasmic viral genomes may be aborted nuclear entry events, rather than intermediates in the nuclear entry pathway.
Second, how might SV40 genomes be targeted to cross the nuclear membrane? The 5.2-kbp circular double-stranded SV40 DNA genome is associated with about 200 copies of cellular histones, all of which contain an NLS. Thus, it is tempting to hypothesize that these histones might mediate nuclear entry of the viral genome via the nuclear transport machinery. However, contrary to that premise, microinjected SV40 genomes, in complex with empty capsids, but not with histones, were reported to enter the nucleus (10).
Results reported here imply that SV40 disassembly intermediates, which contain exposed internal capsid proteins VP2/3, as well as exposed SV40 genomes, arrive at the nuclear periphery, where the genome separates from the capsid components and enters the nucleus without them. Taken together with our earlier findings [6, 7], input SV40 particles undergo discrete disassembly steps during entry that are separated temporally and topologically. First, a partial disassembly of the particles occurs in the ER, which causes internal capsid proteins VP2 and VP3 to become accessible to detection with antibodies. Then, in the cytoplasm, disassembly progresses further to also make the genomic DNA accessible to immune detection, as well as to an EdU-based chemical reaction. Finally, prior to or concomitant with nuclear entry, the viral genome disassociates from capsid components and enters the nucleus without them. Whereas NLSs on exposed VP2/3 may target the SV40 disassembly intermediates to the nuclear periphery, they do not appear to mediate nuclear entry of the viral genome. The means by which the SV40 genome is transported into the nucleus remains to be resolved.
African Green Monkey kidney cells (CV-1) were purchased from ATCC. Dulbecco’s Modified Eagle’s Medium (DMEM), 5-Bromo-2-deoxyuridine (BrdU), Pen-Strep, and bovine serum albumin (BSA) were purchased from SIGMA-ALDRICH (Saint Louis, MO). Fetal Bovine Serum-premium select was from ATLANTA Biologicals (Lawrenceville, GA). Fluoromount-G was from Southern Biotech (Birmingham, AL.). Rabbit anti-VP2/3 was from A. Oppenheim (Jerusalem, Israel), mouse anti-BrdU was from Invitrogen (Carlsbad, California). Mouse and rabbit anti-SV40 large-T antigen were from Calbiochem - EMD4 Biosciences (San Diego, CA). (FITC)-conjugated donkey anti-rabbit IgG (H + L), and (Cy3)-conjugated goat anti-mouse IgG (H + L) were from Jackson ImmunoResearch Laboratories (West Grove, PA).
Viral DNA labeling
Procedure for the incorporation of 5-Bromo-2-deoxyuridine (BrdU) into SV40 genomes was as previously described .
150 μl of BrdU-labeled SV40 stock was absorbed onto CV-1 cells growing in 8-well Lab-Tek chamber slides (Nunc, Rochester, NY) for 1 h at 4°C. After one hour the cells were washed once with DMEM plus 10% FBS. Fresh warm DMEM plus 10% FBS was then added to the wells, and the chamber slides were incubated at 37°C and 5% CO2. Input multiplicity of infection in all experiments was approximately 1 PFU per cell. The infections were stopped by washing the chamber slides three times with 1 × PBS and fixed in 100% methanol at −20°C for 10 minutes.
Control media for all mock-infected controls was prepared to closely mimic infected-cell media as follows. BrdU was added to regular media, which was then applied to non-infected cells. After 10–12 days this media was harvested, dialyzed, and used to mock-infect control cells. Importantly, these mock-infected cells were grown concurrently with and processed identically to their infected counterparts, as previously described .
Fixed samples were washed three times with PBS, and once with 0.01% bovine serum albumin (BSA). All samples that were infected with BrdU-labeled SV40 were then treated with 20 KU (Kunitz units) of DNase (Sigma-Aldrich) per well for 20 min at 37°C. After 20 min, the cells were washed 3 times with 0.01% BSA solution. Samples were then incubated with primary antibodies for 1 h at 37°C and then washed three times with 0.01% BSA. The following primary antibodies were utilized in various combinations with each other: mouse anti-BrdU (1:100), rabbit anti-VP2/3 (1:1,000), mouse anti-SV40 LT (1:200), rabbit anti-SV40 LT (1:100). Following the incubation with primary antibodies, the cell were washed 3 times with 0.01% BSA solution and incubated with secondary antibodies for 50 min at room temperature. Secondary antibodies FITC-conjugated donkey anti-rabbit IgG (H + L) (1:100), and Cy3-conjugated goat anti-mouse IgG (H + L) (1:200) were used in conjunction with the appropriate primaries. After the final wash, the cells were mounted in fluoromount-G.
Immunofluorescence microscopy was carried out as previously described .
DK carried out all experiments. LN conceived of the study. DK and LN participated in its design and coordination. LN prepared the first draft of the manuscript and both authors contributed to its final version.
Simian virus 40
Large T antigen
Nuclear localization signal
Nuclear pore complex.
This work was supported by Public Health Service grant CA100479 from the National Cancer Institute to LN.
- Anderson HA, Chen Y, Norkin LC: Bound simian virus 40 translocates to caveolin-enriched membrane domains, and its entry is inhibited by drugs that selectively disrupt caveolae. Mol Biol Cell. 1996, 11: 1825-1834.View ArticleGoogle Scholar
- Eash S, Querbes W, Atwood WJ: Infection of vero cells by BK virus is dependent on caveolae. J Virol. 2004, 78: 11583-11590. 10.1128/JVI.78.21.11583-11590.2004.PubMedPubMed CentralView ArticleGoogle Scholar
- Mannová P, Forstová J: Mouse polyomavirus utilizes recycling endosomes for a traffic pathway independent of COPI vesicle transport. J Viro. 2003, 77: 1672-1681. 10.1128/JVI.77.3.1672-1681.2003.View ArticleGoogle Scholar
- Pelkmans L, Kartenbeck J, Helenius A: Caveolar endocytosis of simian virus 40 reveals a novel two-step vesicular transport pathway to the ER. Nat Cell Biol. 2001, 3: 473-483. 10.1038/35074539.PubMedView ArticleGoogle Scholar
- Kartenbeck J, Stukenbrok H: Helenius A: Endocytosis of simian virus 40 into the endoplasmic reticulum. J Cell Bio. 1989, 109: 2721-2729. 10.1083/jcb.109.6.2721.View ArticleGoogle Scholar
- Norkin LC, Anderson HA, Wolfrom SA, Oppenheim A: Caveolar endocytosis of simian virus 40 is followed by brefeldin A-sensitive transport to the endoplasmic reticulum, where the virus disassembles. J Virol. 2002, 76: 5156-5166. 10.1128/JVI.76.10.5156-5166.2002.PubMedPubMed CentralView ArticleGoogle Scholar
- Kuksin D, Norkin LC: SV40 disassembly during passage through the endoplasmic reticulum and in the cytoplasm. J Virol. 2012, [Epub ahead of print]Google Scholar
- Calothy G, Hirai K, Delendi V: 5-Bromodeoxyuridine incorporation into simian virus 40 deoxyribonucleic acid. Effects on simian virus 40 replication in monkey cells. Virology. 1973, 55: 329-338. 10.1016/0042-6822(73)90172-4.PubMedView ArticleGoogle Scholar
- Schelhaas M, Malmström J, Pelkmans L, Haugstetter J, Ellgaard L, Grünewald K, Helenius A: Simian Virus 40 depends on ER protein folding and quality control factors for entry into host cells. Cell. 2007, 131: 516-529. 10.1016/j.cell.2007.09.038.PubMedView ArticleGoogle Scholar
- Nakanishi A, Clever J, Yamada M, Li PP H, Kasamatsu H: Association with capsid proteins promotes nuclear targeting of simian virus 40 DNA. Proc Natl Acad Sci USA. 1996, 93: 96-1006. 10.1073/pnas.93.1.96.PubMedPubMed CentralView ArticleGoogle Scholar
- Nakanishi A, Shum D, Morioka H, Otsuka E, Kasamatsu H: Interaction of the Vp3 nuclear localization signal with the importin alpha 2/beta heterodimer directs nuclear entry of infecting simian virus 40. J Virol. 2002, 76: 9368-0377. 10.1128/JVI.76.18.9368-9377.2002.PubMedPubMed CentralView ArticleGoogle Scholar
- Strunze S, Engelke MF, Wang IH, Puntener D, Boucke K, Schleich S, Way M, Schoenenberger P, Burckhardt CJ, Greber UF: Kinesin-1-mediated capsid disassembly and disruption of the nuclear pore complex promote virus infection. Cell Host Microbe. 2011, 10: 210-223. 10.1016/j.chom.2011.08.010.PubMedView ArticleGoogle Scholar
- Li PP, Itoh N, Watanabe M, Shi Y, Liu P, Yang HJ, Kasamatsu H: Association of simian virus 40 vp1 with 70-kilodalton heat shock proteins and viral tumor antigens. J Virol. 2009, 83: 37-46. 10.1128/JVI.00844-08.PubMedPubMed CentralView ArticleGoogle Scholar
- Chromy LR, Oltman A, Estes PA, Garcea RL: Chaperone-mediated in vitro disassembly of polyoma- and papillomaviruses. J Virol. 2006, 80: 5086-5091. 10.1128/JVI.80.10.5086-5091.2006.PubMedPubMed CentralView ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.