Analysis of the PDZ binding specificities of Influenza A Virus NS1 proteins
© Thomas et al; licensee BioMed Central Ltd. 2011
Received: 16 November 2010
Accepted: 19 January 2011
Published: 19 January 2011
The Influenza A virus non-structural protein 1 (NS1) is a multifunctional virulence factor with several protein-protein interaction domains, involved in preventing apoptosis of the infected cell and in evading the interferon response. In addition, the majority of influenza A virus NS1 proteins have a class I PDZ-binding motif at the C-terminus, and this itself has been shown to be a virulence determinant.
In the majority of human influenza NS1 proteins the consensus motif is RSxV: in avian NS1 it is ESxV. Of the few human strains that have the avian motif, all were from very high mortality outbreaks of the disease. Previous work has shown that minor differences in PDZ-binding motifs can have major effects on the spectrum of cellular proteins targeted. In this study we analyse the effect of these differences upon the binding of Influenza A virus NS1 protein to a range of cellular proteins involved in polarity and signal transduction.
The Influenza A virus NS1 protein (non-structural protein 1) is extremely important in the pathology of the virus. It is not a virion component, but is expressed early in infection. It is a multifunctional virulence factor and many of its effects are modulated by activation of PI3K, which it binds via its SH3 domain [1–4].
The influenza A virus NS1 protein has several protein interaction sites, including SH2 and SH3 domains, as well as recognition sites for kinases, including CK2 and MAPK. In addition, over 99% of NS1 proteins isolated have a class 1 PDZ binding motif (PBM) at the C-terminus . PDZ domains are 80-90 amino acid domains that function as docking regions for protein-protein interactions [6, 7], and PDZ-containing proteins were originally thought mainly to act as scaffolding proteins for bringing other proteins in proximity to one another, often at the cell membrane. They are now thought to play a more dynamic role, having various functions in cell polarity and cell signalling, depending upon cell cycle and cellular location of the protein (for overviews see Oncogene (2008) 27, review issue 55).
The importance of the PDZ binding motif (PBM) for influenza virulence was suggested by studies finding, in some cases, that attenuated virulence correlated with C-terminal truncations or extensions of the NS1 protein, either deleting or masking the PBM [8–10]. The avian influenza NS1 protein has recently been shown to interact with a number of PDZ domain-containing proteins including MAGI-1,-2, and -3, Dlg and Scribble . Furthermore, NS1's targeting of Scribble has been shown to relocalise it, concomitantly reducing Scribble-induced apoptosis in infected cells.
We have previously shown that the precise amino acid residues composing the PBM are extremely important in substrate selection [12, 13] and we were therefore interested in analysing these differences between the avian-like and human-like PBMs.
Materials and methods
The pCDNA 3.1 plasmids expressing human and avian wild type NS1 proteins have been described previously  and the Ha, Ah, and Aa mutants were generated in these using the Invitrogen GeneTailor system and verified by sequencing. Oligonucleotides were designed in-house and were synthesised by MWG Biotech AG.
The pCDNA 3.1 plasmids expressing wild type HPV-18 E6 and p53 have been described previously .
In vitro translation
The proteins used in this study were translated in vitro using the TNT rabbit reticulocyte lysate system (Promega). They were radiolabelled with either [35S]-Cysteine or [35S]-Methionine (Perkin Elmer), depending upon the sequence of the protein in question. The levels of translated proteins were assayed by SDS-PAGE followed by phosphorimager analysis.
GST pulldown assays
The other GST constructs were as follows:
GST-Dlg N+2 expresses Dlg amino acids 1-404;
GST-Dlg N+3 expresses Dlg amino acids 1-539;
GST-M1P5 expresses MAGI-1 amino acids 1034-1115;
GST-NTMAGI expresses MAGI-1 amino acids 1-734;
GST-CTMAGI expresses MAGI-1 amino acids 735-1374;
GST-Scrib4PDZ contains Scrib amino acids 616-1490.
The fusion proteins were immobilised on Glutathione-Agarose (Sigma) and incubated with in vitro translated proteins radiolabelled with [35S]-Cysteine or [35S]-Methionine, as described previously [15, 16].
Cells and Transfections
293 cells were maintained in Dulbecco's modified medium supplemented with 10% foetal calf serum, and transfections were performed using the standard calcium phosphate precipitation method .
Interferon induction of STAT1 activation
293 cells were transfected with plasmids expressing human wild type, avian wild type or avian Aa mutant (PDZ non-binding) NS1 proteins or with vector alone. After overnight incubation they were treated with 1 × 104 U/ml Hplc-purified Interferon-α for 5 h before the total protein extract was analysed by SDS-PAGE and Western Blotting.
Activated STAT1 was detected using anti phospho-STAT1-specific antibodies (Cell Signaling), and α-actinin antibody (Santa Cruz) was used as loading control.
Western blots were developed by the ECL enhanced chemiluminescence method (GE Healthcare) according to the manufacturer's instructions.
The human and avian type influenza NS1 proteins differ in PDZ-binding activity
Since the sequence of the NS1 PBM has been shown to affect the virulence of the virus , it was of interest to analyse any differences between the PDZ-binding activities of the human and avian NS1 proteins. A PDZ array assay had previously been reported, using a large number of isolated PDZ domains, and this had identified the PDZ domains of several proteins associated with intercellular membranes, including the Dlg PDZ1 domain .
The NS1 Dlg interaction is PDZ-dependent
Thus the interaction between NS1 and Dlg indeed appears to occur primarily through a PDZ-dependent interaction.
Mapping the the PDZ domain of Dlg targeted by NS1
A number of studies have shown that PDZ-dependent interactions are very specific, with each domain on a multi-PDZ domain protein having specific binding partners [15, 19–22]. The human papillomavirus type 18 E6 protein, for example, binds exclusively to Dlg's PDZ2 domain . Thus, having shown that the interaction between NS1 and Dlg was PDZ-dependent, it was interesting to know how selective the influenza A NS1 might be of specific PDZ domain(s) of Dlg.
The exact PDZ-binding motif sequence directs the specificity of binding to Dlg
Sequence requirements for NS1 interactions with the PDZ domains of MAGI-1 and Scribble
Having defined the interaction of NS1 and Dlg PDZ3, it was interesting to know whether similar constraints applied to the binding of NS1 to other PDZ domains.
The PDZ domain-containing protein, hScrib, has recently been shown to be a PDZ-dependent target of NS1 . Scrib is a partner of Dlg and of HuGL in the tripartite Scrib complex which contributes to polarity regulation in the cell [see [26, 27], for reviews]. As can be seen from Figure 6, the GST-Scrib4PDZ is bound very strongly by the avian NS1, and weakly by the human NS1; but most interestingly the Aa mutant, which has a non-functional PBM, still binds more strongly to GST-Scrib4PDZ than the human wild type, albeit much less than the wild type avian. This indicates that the NS1 protein interaction with Scrib is mainly mediated by the PBM, but that other regions of the protein may also be involved.
NS1 effect upon hScrib's signalling activity
In this study we have dissected the PDZ binding activities of the Avian and Human type NS1 proteins of Influenza A. It is clear from these studies that the Avian type PBM binds PDZ domains more strongly than does the Human type PBM. It also shows that there are interesting differences in their modes of interaction, depending upon the PDZ domain analysed.
Previous work has shown that screening for protein interactions using isolated PDZ domains can be misleading in determining which PDZ domains are the true binding partners of certain proteins. Our assays using the full-length Dlg protein show differences from data published using only isolated domains (5). In addition, our assays shown in Figures 5 and 6 using either the isolated PDZ domains of MAGI-1 or larger portions of the protein would tend to suggest that data obtained from single domain assays should be treated with caution.
We had previously shown by crystallographic and mutational analysis that the specificities of type 1 PDZ-binding interactions are determined by several factors [12, 13]. The sequence of the canonical motif: x-S/T-x-V/L/I is highly influential and we have shown that changing the V to L in otherwise identical PBMs alters target selection [22, 24]. Furthermore, the presence of serine or threonine can affect target selection, even between highly homologous PDZ domains, depending on the hydrophobicity of the PDZ domain's binding groove . The third layer of selectivity is contributed by the non-canonical -4 and -2 amino acid residues (numbering the final residue as -1), and by the residues immediately upstream of the PBM. We have shown that the influence of these residues can be critical in determining PDZ domain preference, and hence substrate selectivity [12, 13].
In this study we have shown that the Avian type NS1 protein binds strongly to the Dlg PDZ3 domain and it might be reasonable to speculate that type 1 PDZ domains of similar sequence might also be targeted by Avian NS1. We have shown that the binding is specific, and this supports the data of Liu et al  who showed the binding between GST-NS1 and HA-tagged Dlg exogenously expressed in 293T cells. They also showed that the binding of NS1 to each of the highly homologous MAGI-1,-2 and -3 proteins is not equally strong, again supporting our findings that the binding selectivity is mutually determined by the sequences of both PDZ domain and ligand.
As seen in Figure 6 the avian NS1 binds to hScrib, largely through PDZ interactions and this agrees with the data of Liu et al., . The absence of a PBM in truncated NS1 proteins increases the expression of IFN  and correlates with attenuated virulence [8–10]. This, together with the finding that TBEV NS5 binds hScrib and impairs IFN-stimulated JAK/STAT signalling, possibly through feedback between STAT and IFN , led us to investigate the effect of a functional PBM upon IFN-induced STAT activation. Our results show that STAT phosphorylation induced by IFNα is reduced in the presence of an hScrib-binding PBM, and is essentially unaffected by the same protein with two point mutations that render the PBM inactive. Clearly, other PDZ domain proteins could also be involved in this activity, although hScrib is a strong candidate, based upon its strength of interaction and previous studies linking hScrib to the regulation of STAT signalling. It seems possible that this function of NS1 is to assist the virus in evading the IFN response to infection. Soubies et al  showed that truncation of NS1 increases IFN induction during infection and Zielecki et al.  have shown that the presence of an avian type PDZ motif can modulate viral replication in a strain and host-dependent manner. These studies underline the importance of the PDZ domain and support the notion that the strength of PDZ interactions is mediated by the precise sequences of the PDZ domain in question and the canonical and non-canonical residues composing and upstream of the PDZ binding motif.
We would like to thank Dr Clayton Naeve for the plasmids expressing the avian and human type NS1 proteins.
We are most grateful to Dr Sergio Tizminetsky and Dr Natasha Skoko of the I.C.G.E.B. Biotechnology Transfer Unit for the Interferon-α.
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