Evaluation of a rapid diagnostic test, NanoSign® Influenza A/B Antigen, for detection of the 2009 pandemic influenza A/H1N1 viruses
- Gyu-Cheol Lee†1,
- Eun-Sung Jeon†2,
- Won-Shik Kim3,
- Dung Tien Le4,
- Jong-Ha Yoo5 and
- Chom-Kyu Chong2Email author
© Lee et al; licensee BioMed Central Ltd. 2010
Received: 3 August 2010
Accepted: 20 September 2010
Published: 20 September 2010
This study evaluated the clinical accuracy and analytical sensitivity of the NanoSign® Influenza A/B antigen kit in detecting 2009 pandemic influenza A/H1N1 viruses. The kit is one of the most popular rapid diagnostic tests for detecting influenza in Republic of Korea.
The NanoSign® Influenza A/B kit resulted in 79.4% sensitivity and 97.2% specificity compared to RT-PCR in the detection of the viruses from 1,023 specimens. In addition, the kit was able to detect two strains of novel influenza viruses, Influenza A/California/12/2009(H1N1) and clinically isolated wild-type novel influenza A/H1N1, both of which are spreading epidemically throughout the world. In addition, the correlation between NanoSign® Influenza A/B test and conventional RT-PCR was approximately 94%, indicating a high concordance rate. Analytical sensitivity of the kit was approximately 73 ± 3.65 ng/mL of the purified viral proteins and 1.13 ± 0.11 hemagglutination units for the cultured virus.
As the NanoSign® Influenza A/B kit showed relatively high sensitivity and specificity and the good correlation with RT-PCR, it will be very useful in the early control of influenza infection and in helping physicians in making early treatment decisions.
The novel influenza A/H1N1 virus has spread to most of the world's populations, and its spread has led to a pandemic alert situation [1–3]. As a result, at the end of 2009, the World Health Organization announced that the novel influenza A/H1N1 had reached pandemic status .
A variety of different diagnostic methods can be used to detect the presence of influenza viruses in respiratory specimens such as nasopharyngeal aspirates, including direct antigen detection tests, virus isolation in cell cultures, and detection of influenza-specific RNA by real-time reverse transcriptase (RT)-polymerase chain reaction (PCR) [5–10]. Albeit the gold standard for the diagnosis of influenza is virus isolation using chicken embryos or tissue culture method, it has the shortcomings such as time consuming and labor intensiveness; it takes between two to 14 days before results are available. Detection of virus-infected cells in nasopharyngeal secretions by direct or indirect immunofluorescent staining is widely used, but it is a difficult and technician-dependent technique still requiring two hours to complete .
For the effective control and treatment of novel influenza, rapid and cost-effective diagnosis is important. Rapid diagnosis of influenza allows the physician to begin antiviral treatment, thereby helping control nosocomial transmission of the infection [12, 13]. It also helps reducing costs and hospital stay. Rapid diagnostic tests (RDT), known as lateral flow rapid tests, have previously been shown to be cost-effective in pediatric patients [14, 15] and effective in controlling influenza epidemics in geriatric institutions [12, 16].
The NanoSign® Influenza A/B test is a rapid diagnostic test, which detects the viral nucleoprotein antigen of influenza virus. This kit has been popularly used in Korea and by Korean governmental organizations, including Korea Centers for disease control and prevention (CDC), since its high accuracy has been demonstrated, with a high sensitivity and specificity against seasonal influenza A viruses, including A/H3N2, A/H1N1 (seasonal) and H2N2 [17, 18].
This study evaluated the clinical accuracy and analytical sensitivity of the NanoSign® Influenza A/B kit in detecting novel influenza A/H1N1. Using two types of novel influenza A/H1N1, A/California/12/2009(H1N1) and clinically isolated wild type influenza A/H1N1, the sensitivity and detection limits of the NanoSign® Influenza A/B kit were evaluated in this study.
Results and Discussion
Performance of NanoSign® Influenza A/B Antigen kit for the detection of novel influenza A/H1N1
Results of conventional RT-PCR
Results of NanoSign® Influenza A/B antigen kit
Detailed results for the detection of novel influenza viruses by institutions
Results of NanoSign® Influenza A/B antigen kit/Results of conventional RT-PCR
(Ilsan city), n = 170
Chungbuk National University Hospital (Cheongju city), n = 123
Local ENT Clinics
(Korean domestic), n = 730
Total, n = 1,023
Values obtained by the NanoSign® Influenza A/B test showed a considerably high accuracy in the detection of novel influenza. Some studies have shown a very low sensitivity (about 50%) using other RDT kits that are predominantly used in the market, even though the kits have a high accuracy for the detection of seasonal influenza viruses [20, 21]. In addition, the correlation between NanoSign® Influenza A/B test and conventional RT-PCR was approximately 94%, indicating a high concordance rate.
Rapid influenza diagnostic tests are antigen-detection tests that target the nucleoprotein of the virus. Usually, the commercially available RDT kits provide results within less than 30 minutes. Thus, results are available in a clinically relevant time frame to help assist in the clinical decision-making process, thereby preventing secondary infection. Some studies have reported that RDT kits have a low to moderate sensitivity against novel influenza compared to RT-PCR . However, the analytical sensitivity of RDT kits is dependent on the properties of its antibody. According to this clinical evaluation of the NanoSign® Influenza A/B antigen kit, its sensitivity for novel influenza (79.4%) was significantly higher than commercialized detection kits (10-70%) that have been reported to the CDC . There are many factors that can affect the sensitivity of the RDT kit, since, unlike RT-PCR, it does not have any target amplification step. Factors that contribute to the sensitivity of the RDT include technician skill in collecting specimens, the type of specimen, the quality of the specimen, the stage of illness when the specimen was collected, and patient age. In addition, the physician's ability to obtain a good specimen is a key factor in obtaining higher sensitivity.
RDT kits such as the NanoSign® Influenza A/B antigen kit are very useful in the early control of influenza infection and in helping physicians in making early treatment decisions. However, the negative results of this particular RDT kit used in this study do not rule out influenza virus infection because its sensitivity is not 100%. For this reason, when there is a high clinical suspicion of influenza infection in a patient, empirical antiviral therapy should be administered. Finally, when reporting the results of a RDT kit, it should be better to inform the physicians about the limitations of the test, so that they can decide the most effective clinical approach.
The NanoSign® Influenza A/B kit had 79.4% sensitivity and 97.2% specificity in the detection of the viruses, and could detect two strains of novel influenza viruses, Influenza A/California/12/2009(H1N1) and wild-type novel influenza A/H1N1. Analytical sensitivity of the kit was approximately 73 ± 3.65 ng/mL for the purified viral proteins and 1.13 ± 0.11 HA units for the cultured virus. In addition, the correlation between the NanoSign® Influenza A/B test and conventional RT-PCR was 94%, indicating a high concordance rate. The NanoSign® Influenza A/B kit may be very useful in the early control of influenza infection and in helping physicians in making early treatment decisions.
Influenza A/California/12/2009(H1N1) was kindly provided by the Laboratory of Virology, Chungbuk National University and wild-type novel influenza A/H1N1 was obtained from the Department of Laboratory Medicine, National Health Insurance Corporation Ilsan Hospital.
A host cell and chicken embryos
A host cell, MDCK, for the culture of the viruses from nasal swabs and aspirates was kindly provided by the laboratory of Professor Chan-Hee Lee. Chicken embryos were purchased from an egg store in Korea (Ochang Mart, Ochang, Republic of Korea).
For the culture of Influenza from the aspirates of nasal swabs, MDCK cells were cultured in DMEM (Invitrogen Corporation, Carlsbad, CA) supplemented with 5% fetal bovine serum (FBS, Invitrogen Corporation) at 37°C and 5% CO2. Before infection, MDCK cells were trypsinized in 1 × 107 cells per T75 flasks (Nalge Nunc International, Rochester, NY). After seeding in 10 mL DMEM plus 5% FBS at 80-90% confluency, plated cells were rinsed with phosphate-buffered saline (PBS, Invitrogen Corporation). Suspended specimens in the flasks were gently shaken for two hours for effective mixing. Cell infections were observed until 80-90% of the cells were floating or lightly attached to the T75 flask (typically 4-5 days post-infection). At that time, harvesting and storage of viral supernatant was performed.
The titer of the influenza A/H1N1 virus was determined by HA assay. First, U-bottom 96 well plates (Nalge Nunc International) were prepared and 50 μL of PBS (pH7.0) was added to each well. Next, after adding 50 μL of original viral solution, they underwent two folds serial dilutions. Finally, 50 μL of 0.5% turkey red blood cells were added and mixed. The mixture was then incubated for approximately 30 minutes at room temperature. The number of positive reactions showing agglutination was observed and recorded to calculate virus titer.
To prepare the nasal swab specimens, a sterile swab was carefully inserted into the nostril with the most secretions under visual inspection. Using a gentle rotation, the swab was pushed until a weak resistance occurred at the level of the turbinate (less than one inch into the nostril). Next, the swab was rotated a few times against the nasal wall. To test the RDT kit, a test strip was inserted into the tube containing the 300 μL of extraction solution and allowed to sit at room temperature prior to testing. After preparing the nasal swab, the sample was inserted into the tube and swirled at least six times while pressing the head against the bottom and side of the tube (the swab head should be rolled and squeezed against the inside of the tube as it is removed). Finally, the test strip was inserted into the tube containing the sample-extracted solution. The results were interpreted after 10 minutes. Some positive results appeared sooner, but results were not read after 30 minutes.
For confirmation of cultured influenza A/H1N1, the viral RNA was isolated from the infected MDCK cells. RT-PCR was performed with the commercialized Influenza A(H1N1) Detection Kit by using primers targeting the novel influenza (GeNet Bio, Nonsan, Republic of Korea). RNA was directly extracted from the specimens or virus cultured-supernatant using the QIAGEN® Viral RNA mini kit (Qiagen, Hilden, Germany). cDNA synthesis was accomplished at 42°C for 30 minutes. Next, the DNA was amplified by 40 cycles of PCR with three steps: denaturation at 94°C for 20 seconds, annealing at 54°C for 20 seconds, and elongation at 72°C for 30 seconds. Finally, an additional elongation step (72°C/5 min) was carried out. The amplified gene products were analyzed with 2% agarose gel electrophoresis. The size of the amplified target was 170 base pair for novel influenza A/H1N1 and 350 base pair for seasonal influenza A/H1N1.
We thank to Chan-Hee Lee, the professor of Chungbuk National University, Republic of Korea, for providing Influenza A/California/12/2009(H1N1).
- Centers for disease control and prevention: Swine influenza A (H1N1) infection in two children - Southern California, March-April 2009. MMWR Morb Mort Wkly Rep 2009, 58: 400-402.
- Dawood FS, Jain S, Finelli L, Shaw MW, Lindstrom S, Garten RJ, Gubareva LV, Xu X, Bridges CB, Ueyki TM, for the Novel Swine-Origin Influenza Virus Investigation Team: Emergence of a novel swine-origin infuluenza A (H1N1) virus in humans. N Engl J Med 2009, 360: 2605-2615. 10.1056/NEJMoa0903810PubMedView Article
- Neumann G, Noda T, Kawaoka Y: Emergence and pandemic potential of swine-origin H1N1 influenza virus. Nature 2009, 459: 931-939. 10.1038/nature08157PubMedPubMed CentralView Article
- World now at the start of 2009 influenza pandemic[http://www.who.int/mediacentre/news/statements/2009/h1n1_pandemic_phase6_20090611/en/]
- Andresen DN, Kesson AM: High sensitivity of a rapid immunochromatographic test for detection of influenza A virus 2009 H1N1 in nasopharyngeal aspirates from young children. J Clin Microbiol 2010, 48: 2658-2659. 10.1128/JCM.00229-10PubMedPubMed CentralView Article
- Chidlow G, Harnett G, Williams S, Levy A, Speers D, Smith DW: Duplex real-time reverse transcription PCR assays for rapid detection and identification of pandemic (H1N1) 2009 and seasonal influenza A/H1, A/H3, and B viruses. J Clin Microbiol 2010, 48: 862-866. 10.1128/JCM.01435-09PubMedPubMed CentralView Article
- Kang X, Jiang T, Li Y, Lin F, Liu H, Chang G, Zhu Q, Qin E, Qin C, Yang Y: A duplex real-time RT-PCR assay for detecting H5N1 avian influenza virus and pandemic H1N1 influenza virus. Virol J 2010, 7: 113. 10.1186/1743-422X-7-113PubMedPubMed CentralView Article
- Pabbaraju K, Wong S, Wong AA, Appleyard GD, Chui L, Pang XL, Yanow SK, Fonseca K, Lee BE, Fox JD, Preiksaitis JK: Design and validation of real-time reverse transcription-PCR assays for detection of pandemic (H1N1) 2009 virus. J Clin Microbiol 2009, 47: 3454-3460. 10.1128/JCM.01103-09PubMedPubMed CentralView Article
- Rouleau I, Charest H, Douvill-Fredet M, Skowronski DM, De Serres G: Field performance of a rapid diagnostic test for influenza in an ambulatory setting. J Clin Microbiol 2009, 47: 2699-2703. 10.1128/JCM.00762-09PubMedPubMed CentralView Article
- Yang Y, Gonzalez R, Huang F, Wang W, Li Y, Vernet G, Wang J, Jin Q: Simultaneous typing and HA/NA subtyping of influenza A and B viruses including the pandemic influenza A/H1N1 2009 by multiplex real-time RT-PCR. J Virol Methods 2010, 167: 37-44. 10.1016/j.jviromet.2010.03.007PubMedView Article
- Landry ML, Cohen S, Ferguson D: Impact of sample type on rapid detection of influenza virus A by cytospin-enhanced immunofluorescence and membrane enzyme-linked immunosorbent assay. J Clin Microbiol 2000, 38: 429-430.PubMedPubMed Central
- Leonardi GP, Leib H, Birkhead GS, Smith C, Costello P, Conron W: Comparison of rapid detection methods for influenza A virus and their value in health-care management of institutionalized geriatric patients. J Clin Microbiol 1994, 32: 70-74.PubMedPubMed Central
- Liao RS, Tomalty LL, Majury A, Zoutman DE: Comparison of viral isolation and multiplex real-time reverse transcription-PCR for confirmation of respiratory syncytial virus and influenza virus detection by antigen immunoassays. J Clin Microbiol 2009, 47: 527-532. 10.1128/JCM.01213-08PubMedPubMed CentralView Article
- Noyola DE, Demmler GJ: Effect of rapid diagnosis on management of influenza A infections. Pediatr Infect Dis J 2000, 19: 303-307. 10.1097/00006454-200004000-00008PubMedView Article
- Woo PC, Chiu SS, Seto WH, Peiris M: Cost-effectiveness of rapid diagnosis of viral respiratory tract infections in pediatric patients. J Clin Microbiol 1997, 35: 1579-1581.PubMedPubMed Central
- Church DL, Davies HD, Mitton C, Semeniuk H, Logue M, Maxwell C, Donaldson C: Clinical and economic evaluation of rapid influenza A virus testing in nursing homes in Calgary, Canada. Clin Infect Dis 2002, 34: 790-795. 10.1086/338960PubMedView Article
- Bioland supply the rapid diagnostic test kit, NanoSign ® Influenza A/B Antigen, to Korea Centers for disease control and prevention[http://www.mdtoday.co.kr/mdtoday/index.html?no=94894]
- Bioland supply the rapid diagnostic test kit, NanoSign ® Influenza A/B Antigen[http://www.wownet.co.kr/news/wownews/view_nhn.asp?bcode=N07010000&artid=A200908190054]
- Altman DG, Bland JM: Diagnostic tests 1: Sensitivity and specificity. BMJ 1994, 308: 1552.PubMedPubMed CentralView Article
- Faix DJ, Sherman SS, Waterman SH: Rapid test sensitivity for novel swine-origin influenza A(H1N1) virus in humans. N Engl J Med 2009, 361: 728-729. 10.1056/NEJMc0904264PubMedView Article
- Ginocchio CC: Evaluation of multiple test methods for the detection of the novel 2009 influenza A(H1N1) during the New York City outbreak. J Clin Virol 2009, 45: 191-195. 10.1016/j.jcv.2009.06.005PubMedView Article
- Sakai-Tagawa Y, Ozawa M, Tamura D, Le QM, Nidom CA, Sugaya N, Kawaoka Y: Sensitivity of influenza rapid diagnostic tests to H5N1 and 2009 pandemic H1N1 viruses. J Clin Microbiol 2010.
- Velasco JMS, Montesa-Develos ML, Jarman RG, Lopez MNB, Gibbons RV, Valderama MTG, Yoon IK: Evaluation of QuickVue influenza A+B rapid test for detection of pandemic influenza A/H1N1 2009. J Clin Virol 2010, 48: 120-122. 10.1016/j.jcv.2010.03.010PubMedView Article
- Interim report on clinical presentation of the novel influenza A (H1N1) cases reported from Kobe City[http://idsc.nih.go.jp/disease/swine_influenza_e/idsc_e2009/clinical_epi_kobe.html]
- Interim guidance for the detection of novel influenza A virus using rapid influenza diagnostic tests[http://www.cdc.gov/h1n1flu/guidance/rapid_testing.htm]
- Reimer CB, Baker RS, Newlin TE, Havens ML: Influenza virus purification with the zonal ultracentrifuge. Science 1966, 152: 1379-1381. 10.1126/science.152.3727.1379PubMedView Article
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.