Bovine ISG15: an antiviral and inducible protein in BIV infected fetal bovine lung cells
© Liu et al; licensee BioMed Central Ltd. 2010
Received: 19 March 2010
Accepted: 23 June 2010
Published: 23 June 2010
Bovine ISG15 (bISG15) is an interferon inducible ubiquitin-like protein that is responsible for the establishment of early pregnancy in ruminant, understanding the properties of bISG15 capable of being inducible in fetal bovine lung (FBL) cells upon infection of bovine immunodeficiency virus (BIV) is of significant importance. In this study, we investigated the expression of bISG15 in poly I:C treated FBL cells. The increased expression of bISG15 was observed, and the inhibition of BIV replication was also detected in FBL cells. Elimination of bISG15 expression by small interfering RNA reversed the bISG15 mediated inhibition of BIV replication. These findings demonstrate that bISG15 plays an important role in inhibition of the BIV replication in FBL cells. Furthermore, real-time PCR and western blot assay revealed that bISG15's expression can also be induced in BIV infected FBL cells. Taken together, bISG15 is an antiviral and inducible protein in BIV infected FBL cells.
Type I IFNs' binding to cell type specific receptors can induce the synthesis of a plethora of proteins , many of which play crucial roles in the antiviral response . ISG15 was one of the most strongly induced proteins. Thirty years past since ISG15 was first found , whereas its antiviral function was reported recently . ISG15, a 15-kDa ubiquitin-like protein, can be covalently conjugated to target proteins for post-translational modification through a series of steps similar to ubiquitin modification . ISG15 and its conjugation modification play roles in processes including regulation of IFN signaling, innate immunity, anti-viral infections, pregnancy, and carcinogenesis . Interestingly, ISG15 and enzymes involved in ISG15 modification are interferon inducible [1, 3, 6–8]. This indicates ISG15 and its conjugation play important roles in innate immunity and antiviral response to infection. Mice deficient of ISG15 have been shown to be more susceptible to influenza, herpes, and Sindbis viruses' infection . It has been reported that ISG15 can inhibit the release of human immunodeficiency virus type 1 (HIV-1) virions by disrupting the interaction of the Gag L domain with Tsg101 , and ISG15 can also repress the replication of Ebola virus by inhibiting the release of virions . Unlike ubiquitin or other ubiquitin-like molecules, cross-species' conservation of ISG15 is low . Therefore, it is necessary and intriguing to elucidate the antiviral functions of ISG15 in wide variety of species.
Bovine ISG15 (bISG15) has 70% homology amino acid sequence to human ISG15 . The studies about bISG15 are mainly focused on its role in reproductive biology. The expression of bISG15 is up-regulated at endometrium in response to conceptus-secreted IFN-τ during early pregnancy . The relation between bISG15 and innate immune response has also been noticed. The expression of bISG15 in maternal blood is increased in cows infected with acute non-cytopathic bovine viral diarrhea virus (BVDV) . However, the reports about property of the bISG15 with antiviral function are scarce up to date. Recently, we have found that expression of bISG15 can be induced in fetal bovine lung (FBL) cells after treated with poly I:C or lipopolysaccaride (LPS) . FBL cells were chosen to be as a target type of cells here, since our lab found that FBL cells could be infected by viruses which are capable of infecting cattle, such as BVDV, bovine foamy virus (BFV), and bovine immunodeficiency virus (BIV). BIV can infect FBL cells in vitro, and induce syncytia formation in FBL cells. Moreover, BIV DNA can also be detected in bovine lung tissues . BIV, which has a similar genome to HIV-1, is associated with chronic inflammatory diseases in infected cows . Considering the similarity between BIV and HIV-1, BIV may contribute as alternative animal virus model for certain aspects of HIV research. It has been reported that ISG15 can inhibit the replication of HIV . We previously demonstrated that bISG15 can be induced robustly in poly I:C treated FBL cells . Here, we further try to address whether the replication of BIV can be repressed in FBL cells treated with poly I:C and to address extensively whether the expression of bISG15 is changed in FBL cells infected with BIV.
In this study, FBL cells were isolated from fetal bovine lung issues. The primary FBL cells isolated from the lung issues of fetal calf were called passage"1". When FBL cells proliferated and separated, they were called passage "2" and so on. All the FBL cells used in the experiment were the passages between 10 and 20. BIV indicator cell line, designated as BIVL, has an integrated reporter plasmid which has a firefly luciferase reporter protein gene downstream of BIV LTR promoter . FBL cells and BIVL cells were maintained in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 50 IU/ml penicillin, 50 μg/ml streptomycin in 5% CO2 at 37°C. Due to fail of obtaining cell free BIV, a BIV strain R29 was used here. The BIV R29 strain was maintained in FBL cells. All the BIV used in the experiment were the FBL cells infected BIV. Poly I:C (purchased from Sigma-Aldrich) was dissolved in PBS at a concentration of 2.5 mg/ml. BIVL cells were plated at a density of 4 × 104 per well in 12-well plates to indicate BIV infection efficiency. BIVL cells co-cultured with BIV infected/uninfected FBL cells at equal cell number. After 12 hours, all cells were assessed by luciferase assays (Promega luciferase assay kit) to indicate the replication efficiency of BIV in FBL cells . Synthetic siRNA of bISG15 (bISG15siRNA) and negative control siRNA (scrambled siRNA) (purchased from GenePharma) were used as annealed oligonucleotide and was resuspended in RNase-free H2O, respectively. The sense sequence of scrambled siRNA is 5'-UUC UCC GAA CGU GUC ACG UTT-3', the anti-sense sequence of scrambled siRNA is 5'-ACG UGA CAC GUU CGG AGA ATT-3'; and the sense sequence of bISG15siRNA is 5'-CAC CGU GUU CAU GAA UCU ATT-3', the anit-sense sequence of bISG15siRNA is 5'-UAG AUU CAU GAA CAC GGU GTT-3'. To validate the suppressing effect of bISG15, bISG15siRNA or scrambled siRNA (100 Nmol/hole) was transfected to FBL cells with Lipofectamine 2000 (Invitrogen), respectively. Realtime PCR and Western blot assay of bISG15 were performed as previously described . Briefly, total RNA from cells was isolated with TRIzol reagent (Invitrogen) following the manufactures's instructions, and then the extracted RNA was reverse transcribed into cDNA by reverse transcriptase (Promega). Real time PCR was performed using an IQ5 Multicolor Real-time PCR detection system (Bio-Rad) and fluorescent EVAGreen nucleic acid stain (Biotium). Primers used to quantify bISG15's cDNA were bISG15-F (5'-GTG GTG CAG AAC TGC ATC TC-3') and bISG15-R (5'-GCC AGA ACT GGT CTG CTT GT-3'). GAPDH cDNA served as endogenous control, GAPDH was amplified using the primers bGAPDH-F (5'-AAC GGC ACA GTC AAG GCA GA-3') and bGAPDH-R (5'-TCG GCA GAA GGT GCA GAG AT-3'). The level of bISG15 transcription was normalized to GAPDH using the 2-ΔΔCt method. The polyclonal murine bISG15 antibody used in western blot assays was obtained in our lab. Western blot detection was performed using the chemiluminescence detection regent (Santa Cruz). To ensure equal protein loading, anti-β-actin antibody (Santa Cruz) was used to detect β-actin expression in cells.
This study demonstrates that bISG15 is an antiviral and inducible protein in BIV infected fetal bovine lung cells. BIV infection can induce the expression of bISG15 in FBL cells, but it is still unclear that the detail involved in this process. It was shown in Figure 3.a, the induction of bISG15 in FBL cells infected with BIV was decreased at 3 hours post-infection, while FBL cells infected with BIV at 18 hours post-infection were still alive, not dead obviously. To infect the naive FBL cells, we added the BIV infected FBL cells to naive FBL cells. All cells involved in this experiment were exclusively FBL cells not other cells. FBL cells infected with BIV may activate some signal pathways like interferon, NF-κB signal pathway and so on. These signal pathways may induce the expression of bISG15 directly or some cytokines such as Type I interferon. These cytokines then induce the expression of bISG15. It indicates that the induction of bISG15's expression in FBL cells by BIV infection may be related to several viral and cellular events via unknown mechanism. Our results suggested that bISG15 played a role in poly I:C-induced inhibition of BIV in FBL cells. The function of bISG15 repressing the replication of BIV in FBL cells demonstrates that bISG15 is a key mediator induced in FBL cells inhibition of BIV replication and also reveals the antiviral function of ISG15 in other species.
The R29 strain of BIV was kindly provided by Dr Charles Wood (University of Nebraska Lincoln). This work was supported by the National Natural Science Foundation of China (30770081) and Tianjin municipal science and technology foundation (08ZCGHHZ01800).
- de Veer MJ, Holko M, Frevel M, Walker E, Der S, Paranjape JM, Silverman RH, Williams BR: Functional classification of interferon-stimulated genes identified using microarrays. J Leukoc Biol 2001, 69: 912-920.PubMedGoogle Scholar
- Stark GR, Kerr IM, Williams BR, Silverman RH, Schreiber RD: How cells respond to interferons. Annu Rev Biochem 1998, 67: 227-264. 10.1146/annurev.biochem.67.1.227PubMedView ArticleGoogle Scholar
- Farrell PJ, Broeze RJ, Lengyel P: Accumulation of an mRNA and protein in interferon-treated Ehrlich ascites tumour cells. Nature 1979, 279: 523-525. 10.1038/279523a0PubMedView ArticleGoogle Scholar
- Dao CT, Zhang DE: ISG15: a ubiquitin-like enigma. Front Biosci 2005, 10: 2701-2722. 10.2741/1730PubMedView ArticleGoogle Scholar
- Haas AL, Ahrens P, Bright PM, Ankel H: Interferon induces a 15-kilodalton protein exhibiting marked homology to ubiquitin. J Biol Chem 1987, 262: 11315-11323.PubMedGoogle Scholar
- Kim KI, Giannakopoulos NV, Virgin HW, Zhang DE: Interferon-inducible ubiquitin E2, Ubc8, is a conjugating enzyme for protein ISGylation. Mol Cell Biol 2004, 24: 9592-9600. 10.1128/MCB.24.21.9592-9600.2004PubMedPubMed CentralView ArticleGoogle Scholar
- Moynihan TP, Ardley HC, Nuber U, Rose SA, Jones PF, Markham AF, Scheffner M, Robinson PA: The ubiquitin-conjugating enzymes UbcH7 and UbcH8 interact with RING finger/IBR motif-containing domains of HHARI and H7-AP1. J Biol Chem 1999, 274: 30963-30968. 10.1074/jbc.274.43.30963PubMedView ArticleGoogle Scholar
- Johnson GA, Joyce MM, Yankey SJ, Hansen TR, Ott TL: The Interferon Stimulated Genes (ISG) 17 and Mx have different temporal and spatial expression in the ovine uterus suggesting more complex regulation of the Mx gene. J Endocrinol 2002, 174: R7-R11. 10.1677/joe.0.174R007PubMedView ArticleGoogle Scholar
- Lenschow DJ, Lai C, Frias-Staheli N, Giannakopoulos NV, Lutz A, Wolff T, Osiak A, Levine B, Schmidt RE, Garcia-Sastre A, et al.: IFN-stimulated gene 15 functions as a critical antiviral molecule against influenza, herpes, and Sindbis viruses. Proc Natl Acad Sci USA 2007, 104: 1371-1376. 10.1073/pnas.0607038104PubMedPubMed CentralView ArticleGoogle Scholar
- Okumura A, Lu G, Pitha-Rowe I, Pitha PM: Innate antiviral response targets HIV-1 release by the induction of ubiquitin-like protein ISG15. Proc Natl Acad Sci USA 2006, 103: 1440-1445. 10.1073/pnas.0510518103PubMedPubMed CentralView ArticleGoogle Scholar
- Okumura A, Pitha PM, Harty RN: ISG15 inhibits Ebola VP40 VLP budding in an L-domain-dependent manner by blocking Nedd4 ligase activity. Proc Natl Acad Sci USA 2008, 105: 3974-3979. 10.1073/pnas.0710629105PubMedPubMed CentralView ArticleGoogle Scholar
- Perry DJ, Austin KJ, Hansen TR: Cloning of interferon-stimulated gene 17: the promoter and nuclear proteins that regulate transcription. Mol Endocrinol 1999, 13: 1197-1206. 10.1210/me.13.7.1197PubMedView ArticleGoogle Scholar
- Hansen TR, Austin KJ, Johnson GA: Transient ubiquitin cross-reactive protein gene expression in the bovine endometrium. Endocrinology 1997, 138: 5079-5082. 10.1210/en.138.11.5079PubMedView ArticleGoogle Scholar
- Smirnova NP, Bielefeldt-Ohmann H, Van Campen H, Austin KJ, Han H, Montgomery DL, Shoemaker ML, van Olphen AL, Hansen TR: Acute non-cytopathic bovine viral diarrhea virus infection induces pronounced type I interferon response in pregnant cows and fetuses. Virus Res 2008, 132: 49-58. 10.1016/j.virusres.2007.10.011PubMedView ArticleGoogle Scholar
- Liu C, Chang R, Yao X, Qiao WT, Geng YQ: ISG15 expression in response to double-stranded RNA or LPS in cultured Fetal bovine lung (FBL) cells. Vet Res Commun 2009, 33: 723-733. 10.1007/s11259-009-9221-8PubMedView ArticleGoogle Scholar
- Gonda MA, Oberste MS, Garvey KJ, Pallansch LA, Battles JK, Pifat DY, Bess JW Jr, Nagashima K: Development of the bovine immunodeficiency-like virus as a model of lentivirus disease. Dev Biol Stand 1990, 72: 97-110.PubMedGoogle Scholar
- St-Louis MC, Cojocariu M, Archambault D: The molecular biology of bovine immunodeficiency virus: a comparison with other lentiviruses. Anim Health Res Rev 2004, 5: 125-143. 10.1079/AHR200496PubMedView ArticleGoogle Scholar
- Yao X, Su Y, Liu C, Tan J, Liu L, Geng YQ, Qiao WT: Establishment of an indicator cell line for monitoring bovine immunodeficiency virus infection and inhibitor susceptibility. J Virol Methods 2010, 163: 25-30. 10.1016/j.jviromet.2009.07.007PubMedView ArticleGoogle Scholar
- Sen GC, Sarkar SN: The interferon-stimulated genes: targets of direct signaling by interferons, double-stranded RNA, and viruses. Curr Top Microbiol Immunol 2007, 316: 233-250. full_textPubMedGoogle Scholar
- Meylan E, Tschopp J: Toll-like receptors and RNA helicases: two parallel ways to trigger antiviral responses. Mol Cell 2006, 22: 561-569. 10.1016/j.molcel.2006.05.012PubMedView ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.