Posttranslational N-glycosylation of the hepatitis B virus large envelope protein
© Lambert and Prange; licensee BioMed Central Ltd. 2007
Received: 22 March 2007
Accepted: 30 May 2007
Published: 30 May 2007
The addition of N-linked glycans to proteins is normally a cotranslational process that occurs during translocation of the nascent protein to the endoplasmic reticulum. Here, we report on an exception to this rule occurring on the hepatitis B virus (HBV) large L envelope protein that is a subject to co-plus posttranslational N-glycosylation.
By using an improved detection system, we identified so far unrecognized, novel isoforms of L. Based on mutational analyses, the use of N-glycosylation inhibitors, and pulse-chase studies, we showed that these isoforms are due to posttranslational N-glycan addition to the asparagines 4 and 112 within the preS domain of L. While an inhibition of N-glycosylation and glycan trimming profoundly blocked virus assembly and release, the posttranslational N-glycosylation of L itself was found to be dispensable for HBV morphogenesis.
These data together with previous results implicate that the N-glycosylation requirements of virion release are due to functional inhibition of cell glycoproteins engaged by HBV.
N-glycosylation is known to possess important functions for many proteins, as it affects protein folding, quality control, sorting, degradation, secretion, and modulates immune responses [1, 2]. N-linked glycans are added to proteins en bloc in the lumen of the endoplasmic reticulum (ER) as presynthesized oligosaccharides. The reaction is catalyzed by the oligosaccharyltransferase (OST)-complex that links the glycan unit to the asparagine residue in the target sequence N-X-T/S. As the OST-complex is associated with the translocon, N-glycosylation normally occurs cotranslationally when the nascent protein chain grows into the ER lumen [1, 2]. However, in the present study we show that the large envelope protein of the hepatitis B virus (HBV) is also modified by posttranslational N-glycosylation.
HBV formation strictly depends on a functional N-glycosylation apparatus of the cell, since both the inhibition of N-glycosylation and N-glycan processing by ER glucosidases block virion egress [12–14]. By contrast, the release of empty envelope subviral particles that are mainly formed by the S protein and known to accompany HBV morphogenesis does not require N-glycans [6, 13]. Based on these observations, the preS2-specific N-glycan of the M protein was suggested to be the prime target for N-glycosylation inhibitors that would provoke aberrantly folded M polypeptides with the potential to block HBV envelope and hence virus maturation . Consistent with this, a mutational inactivation of the preS2-specific N-glycosylation sequon of M has been shown to prevent virus release in one report . Inconsistently, however, several studies have documented that M is dispensable for HBV morphogenesis [15–17] and thus leave the puzzle why virus production may require the preS2-linked glycan but does not need the M protein.
In this study we readdressed the role of N-glycosylation in HBV maturation with special emphasis on the L envelope protein. We provide first evidence that L is N-glycosylated within its preS1 and preS2 domains in an uncommon posttranslational manner. In order to solely examine the impact of the preS-specific glycosylation of L on virus production, we used an engineered in vitro-replication system that allows to study HBV assembly in the absence of an ongoing M protein synthesis. We found that mutational inactivation of the L-specific glycosylation sites did not affect virus maturation, whereas inhibitors of N-glycosylation and glycan trimming blocked virus production, thus indicating that proper N-glycosylation and processing of host cell components are involved in HBV multiplication.
Detection of two novel L forms
Novel L forms result from N-glycosylation
By using protease protection assays, we observed that the novel L forms were resistant against proteolysis and hence should have their preS domains orientated into the ER lumen (data not shown). Therefore, we reasoned that the higher molecular weights of the 48 kDa and 51 kDa L derivatives might be due to modification with N-glycans attached to the preS region that contains two potential consensus motifs (N4 and N112; Fig. 1A). To test for N-glycosylation, lysates of L-expressing HEK 293T cells were treated with Peptide:N-Glycosidase F (PNGase F), an enzyme that cleaves high mannose, hybrid and complex oligosaccharides from N-linked glycoproteins. As shown in Fig. 2B, PNGase F not only converted gp42 but also the two additional forms to the nonglycosylated p39 form of L. To verify these results, we analyzed the L expression profile in the presence of tunicamycin, which prevents N-glycosylation. As expected, treatment of L-expressing HEK 293T cells with tunicamycin inhibited the formation of gp42, but, importantly, also the generation of the 48 kDa and 51 kDa forms. Although these data pointed to differently N-glycosylated L chains, the 48 kDa and 51 kDa forms could equally represent a single-glycosylated L polypeptide carrying distinct oligosaccharide moieties due to processing of glycan structures in the Golgi stacks. To examine whether the L forms had acquired complex glycan chains, cellular extracts were treated with endoglycosidase H (Endo H) which cleaves N-glycans from the high-mannose-type present on glycoproteins in the ER, while glycans trimmed within the Golgi to complex/hybrid structures are resistant to Endo H. This analysis clearly revealed that all modified L forms were converted by Endo H to the non-glycosylated p39 L polypeptide (Fig. 2B), thus indicating that they did not resemble Golgi-processed glycan derivatives. Together, these data demonstrated additional N-glycosylation of L occurring beyond its known glycosylation site at N309.
Novel L forms result from N-glycosylation within preS1 and preS2
N-glycosylation within preS1 and preS2 occurs posttranslationally
Inhibition of N-glycosylation in the absence of the M protein inhibits HBV release
Inhibition of posttranslational N-glycosylation of L does not inhibit HBV release
This prompted us to investigate whether posttranslational preS N-glycosylation of L per se is involved in HBV morphogenesis. As above, the HBV genomic construct defective in envelope protein synthesis (HBV.env-) was cotransfected together with wild-type or mutant S and Lo expression constructs in HuH-7 cells and intracellular nucleocapsid assembly and release of virions into the medium were monitored by the EPR assay. Because the preS2-specific glycan acceptor residue (N112) of L is located within a critical region involved in nucleocapsid envelopment comprising amino acids 92 to 113 (corresponding to 103 to 124 in HBV, subtype adw2) [17, 22], we created the substitution T114A that destroyed the glycosylation consensus motif (N-X-S/T) without being part of the nucleocapsid envelopment region. While the Lo.N112Q variant had a negative phenotype as expected, the Lo.T114A mutant still supported virus production and thus even more efficiently as compared to the wild-type Lo protein (Fig. 5B). Similarly, inactivation of the preS1-specific glycosylation site of L had no inhibitory effect on virus formation, neither alone (Lo.N4Q) nor in combination with the preS2 glycan knockout mutation (Lo.N4Q, T114A) (Fig. 5B). In accordance with published data  we also found no influence on virion secretion when the glycan acceptor residue in the S protein (N146Q) and in the S domain of L (N309Q) was mutated simultaneously (data not shown). Together, these results indicated that posttranslational N-glycosylation of L as well as cotranslational N-glycosylation of L and S are not required for HBV maturation. Hence, the observed inhibitory effects of the N-glycosylation-interfering drugs on virus production are likely due to functional inhibition of host cell factors engaged by HBV for its propagation.
In this work we report that the HBV L envelope protein is modified by N-glycosylation within its N-terminal preS1 and preS2 domains. As would be expected from the unique L biogenesis and topology, these modifications take place after completion of L synthesis and thus in a posttranslational mode. In general, N-glycans are cotranslationally transferred to the asparagine residue of the sequon N-X-S/T as the growing polypeptide is translocated from the ribosome into the ER lumen [1, 2]. However, recent reports provided first hints for an exception to this rule in such that N-glycans can be also added posttranslationally to polypeptides at least under artificial conditions. For examples, posttranslational N-glycosylation had been observed for the aglycoinsulin receptor synthesized in the presence of tunicamycin , for a truncated form of the peptidylglycine α-amidating monooxygenase , or in manosylphosphoryldolichol-deficient mutant cells . The human coagulation factor VII is another substrate of posttranslational N-glycosylation which becomes modified within its very C-terminal region, presumably because conformational constraints do not favour a cotranslational N-glycan addition . In the case of the HBV L protein, the posttranslationally N-glycosylated sites are located in its N-terminal domain but first entered the ER lumen after chain termination [9, 11, 18]. Hence, the data presented here pointed out the potential of the cellular machinery to conduct N-glycan attachment independently of protein translation. Although the enzymes catalyzing the posttranslational N-glycosylation of L remain to be characterized, it is tempting to speculate that the cellular OST-complex is responsible for this modification based on our mutagensis analysis, the use of endoglycosidases and the use of tunicamycin, an inhibitor of the OST-driven N-glycosylation reaction. Accordingly, completed L polypeptides that posttranslationally translocate their preS domains into the ER should still have access to the OST-complex even after their uncoupling from the ribosome. In support of this view, recent data suggested that the OST-complex might associate with the translocon in either a ribosome-dependent or ribosome-independent manner . Therefore, it seems possible that proteins which remain transiently associated with the Sec61 translocon after chain termination or even reassociate with this channel can become N-glycosylated by OST. Based on pulse-chase analysis, we have evidence that the HBV L protein indeed is associated with the translocon for a prolonged time (our unpublished observations), thus rendering this protein as a model substrate to further investigate the mechanism(s) of posttranslational N-glycosylation uncoupled from protein synthesis.
Because the L protein along with its split topology is a key player in the HBV life cycle, and because N-glycans have been shown to serve as folding and trafficking devices of many glycoproteins [1, 2], it appeared reasonable to assume that the newly identified posttranslational N-glycosylation of L may contribute to viral replication. Previous studies have well documented an extreme sensitivity of HBV egress to inhibition of N-glycosylation and ER glucosidase function. Mutational analyses of the S-specific N-glycan site, modified in all three envelope proteins, revealed that this site is dispensable for viral particle release and therefore should not be targeted by N-glycosylation-interfering drugs. Rather, the preS2-specific N-glycan of M was claimed to be responsible for N-glycan-dependent HBV maturation, as an inactivation of this sequon by a single mutation (N-X-T to N-X-A) blocked virus release . Conversely, however, in two other reports a disruption of this sequon showed no measurable effects on virus production [17, 22]. With regard to these conflicting data of the role of the preS2-specific N-glycan and of the M protein per se played during virus formation [15, 16], here we have examined the impact of posttranslational N-glycosylation of L in HBV-replicating cells in which M protein synthesis was blocked. A treatment of these cells with the glucosidase inhibitors CST or NB-DNJ significantly reduced HBV release without, notably, affecting intracellular nucleocapsid assembly, implicating that these drugs interfered with proper L protein folding, trafficking and/or nucleocapsid envelopment. To our surprise, however, the mutational inactivation of the preS-specific N-glycosylation sites of L, either individually or in combination, did not diminish virus production. Together, these results suggested that posttranslational N-glycosylation of L itself is not necessary for HBV morphogenesis. Hence, the N-glycosylation requirements of virion release are likely due to modifications of cell glycoproteins assisting in L envelope and/or virus maturation. The search for such proteins is under active investigation.
Rather, the posttranslationally added N-glycans may serve other, yet to be determined functions in the HBV infection cycle. Interestingly, in an early study characterizing the L protein in secreted HBV particles, Heermann and co-workers also detected protein bands of > 45 kDa in purified virus samples but not in preparations of subviral particles . Based on this observation it is tempting to speculate that L proteins with posttranslational glycan additions in the preS-domain may be selectively incorporated into mature virion particles. With respect to L topogenesis, these glycan chains presumably are exposed on the virus surface where they may contribute to virulence. First experiments analyzing the glycosylation pattern of secreted viral particles confirmed the existence of additional preS1-specific N-glycans (data not shown). For several viruses, like HIV-1, Sindbis virus, Dengue virus, human cytomegalovirus, hepatitis C virus, and Ebola virus, the N-linked glycans in their envelope glycoproteins have been shown to mediate host-cell recognition , and ref. therein]. Therefore, one putative function of the preS-linked glycans may reside in facilitating HBV binding to liver cells. In support of this view, a deletion of amino acids 3–7 of L, including the preS1 N-glycan sequon, has been shown to block HBV infection of primary human hepatocytes . Synergistically, the preS-linked glycans may protect the surface-exposed preS domain of L along with its essential receptor binding site for degradation, thereby enhancing virus stability and supporting its infectivity. It is equally possible that these glycans may provide a means to combat humoral immune surveillance in such that they may form a formidable barrier for development of strong antibody responses to HBV.
The mammalian expression vectors carrying the HBV (subtype ayw) L gene (pNI2.L) or S gene (pNI2.S) under the control of the human metallothionein IIA promoter have been described . To abolish concomitant expression of the HBV M and S proteins from the L open reading frame, their translational start codons had been inactivated in the plasmid pNI2.Lo as described .
Site-directed mutagenesis was performed using the QuickChange II XL kit (Stratagene) as recommended by the manufacturer. For simplification, amino acid numbering used herein will refer to consecutive numbering of the L protein rather than to the envelope protein domains (see Fig. 1A). The N112Q mutation was introduced with the forward primer 5'-CATCCTCAGGCCCTGCAGTGGCAAT CCACAACCTTC-3' (nucleotides (nts) 1210–1245, mutations are in boldface) and a complementary reverse primer. For the T114A mutation, the primer 5'-CCATGCAGTGGAACTCCGCAACCTTCCACC-3' (nts 1220–1249) and its corresponding reverse primer were used. The N4Q mutation was introduced by PCR in a two-step reaction. First, a 150 bp product was generated using the 5'-primer 5'-CCTCCTCCAAGTCCCAGCGAACCC-3' (nts 772–795) and the mutagenic 3'-primer 5'-GCTGGTGGACAGCTGCTGCCCCAT-3' (nts 895–921). In a second reaction, this product served as a 5'-primer together with the 3'-primer 5'-TGTGATGTTCTCCATATGCAGCGC-3' (nts 1378–1401). The resulting product was cut with SacI and XhoI and used to substitute for the wild-type fragment of pNI2.Lo. The mutation of N309Q within the S coding region (corresponding to N146Q of S) has been described . A XhoI-SpeI-fragment containing the N309Q mutation (nts positions 1359–19011) was subcloned into the pNI2.Lo vector.
Cell culture, transfection, and lysis
In order to rule out cell type-specific effects on protein biogenesis, we used transient expression of L in different cell lines. COS-7 cells, HEK 293T cells, and HuH-7 liver cells were transfected using electroporation, Lipofectamine 2000 reagent (Invitrogen), or Lipofectamine/Plus reagents (Invitrogen), respectively, as recommended by the manufacturers. In addition, L synthesis and maturation was examined in the cell line EcR-LHA-293  which stably expresses L under the control of the ecdysone-inducible promoter. Unless otherwise indicated, cell lysates were prepared by incubating the cells with lysis buffer (100 mM Tris-HCl (pH 8.0), 100 mM NaCl, 10 mM EDTA, 1% NP-40) for 15 min on ice and cleared by centrifugation at 10,000 g for 5 min at 4°C. Alternatively, cells were disrupted by homogenization and microsomal fractions were prepared by ultracentrifugation essentially as described .
Glycosidase and tunicamycin treatment
For Peptide:N-Glycosidase F (PNGase F) treatment, cell lysates were boiled for 10 min in denaturing buffer (0,5% SDS, 40 mM DTT), and sodium phosphate (pH 7.5) and NP-40 were added to give a final concentration of 50 mM and 1%, respectively. Samples were divided in two portions and incubated for 1 h at 37°C with or without 1,000 U PNGase F (New England Biolabs). For endoglycosidase H (Endo H) digestion, cells were lysed in 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0,5% SDS, 1% 2-mercaptoethanol and boiled for 10 min. Sodium acetate (pH 5.0) was added at a final concentration of 50 mM and split samples were incubated over night at 37°C with or without 5 mU Endo H (Roche). For the tunicamycin experiment, EcR-LHA-293 cells were treated with 10 μM of the inducer Ponasterone A (PonA, Sigma) for 15 h in the presence or absence of 5 μg/ml tunicamycin (Sigma).
To pulse induce L protein synthesis, EcR-LHA-293 cells were incubated with 10 μM PonA for 3 h. After removal of the inducer, the cells were chase treated with 0,25 mM cycloheximide (ICN biochemicals) for different time periods. For preparation of microsomes, cells were washed twice with Tris-buffered saline (TBS, 50 mM Tris-HCl (pH 7.5), 150 mM NaCl) and incubated for 15 min on ice with 0,1 × TBS-buffer. Microsomes were prepared by homogenisation and ultracentrifugation as described . The microsomal pellet was lysed in loading buffer and subjected to SDS-PAGE and immunoblotting with an L-specific monoclonal antibody recognizing an epitope within the preS1 domain of L (MA18/7) .
Drug treatment of HBV-replicating cells
For replication of HBV in the HuH-7 liver cell line, plasmid HBV.env- was used carrying an 1.1-mer of the HBV DNA genome  in which the translational start codons for the L, M, and S envelope protein had been inactivated by mutagenesis . To allow virus production, pNI2-based plasmids encoding HBV envelope proteins were complemented in trans. For cotransfections, either plasmids pNI2.S plus pNI2.Lo or pNI2.S plus glycosylation mutants of Lo were employed. Cells were left untreated or treated for 70 h with 100 μg/ml N-butyl-deoxynojirimycin (NB-DNJ, Sigma), 1 mM castanospermine (CST, Applichem), or 5 μg/ml tunicamycin. Thereafter, cell culture supernatants were harvested and measured for the release of subviral/viral particles by ELISA (Auszyme, Abbott). In parallel, intracellular nucleocapsids and extracellular virions were isolated by capsid- or envelope-specific immunoprecipitations, respectively, prior to detection of the encapsidated viral progeny DNA by radioactive labelling of the partially double-stranded genome with 10 μCi [α32P]dATP (GE Healthcare) by the endogenous polymerase as described . After extraction of the labelled DNA genomes from the immunoprecipitated samples, they were resolved on agarose gels and visualized by PhosphorImaging. Band-intensities were quantified using the ImageQuant software (Molecular Dynamics).
We thank T. Döring for expert technical assistance and K.-H. Heermann for generously providing the MA18/7 antibody. This work was supported by grants to R.P. from the Deutsche Forschungsgemeinschaft (SFB 490-D1, RP 305/1-3).
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