Feline aminopeptidase N is not a functional receptor for avian infectious bronchitis virus
© Chu et al. 2007
Received: 15 January 2007
Accepted: 26 February 2007
Published: 26 February 2007
Coronaviruses are an important cause of infectious diseases in humans, including severe acute respiratory syndrome (SARS), and have the continued potential for emergence from animal species. A major factor in the host range of a coronavirus is its receptor utilization on host cells. In many cases, coronavirus-receptor interactions are well understood. However, a notable exception is the receptor utilization by group 3 coronaviruses, including avian infectious bronchitis virus (IBV). Feline aminopeptidase N (fAPN) serves as a functional receptor for most group 1 coronaviruses including feline infectious peritonitis virus (FIPV), canine coronavirus, transmissible gastroenteritis virus (TGEV), and human coronavirus 229E (HCoV-229E). A recent report has also suggested a role for fAPN during IBV entry (Miguel B, Pharr GT, Wang C: The role of feline aminopeptidase N as a receptor for infectious bronchitis virus. Brief review. Arch Virol 2002, 147:2047–2056.
Here we show that, whereas both transient transfection and constitutive expression of fAPN on BHK-21 cells can rescue FIPV and TGEV infection in non-permissive BHK cells, fAPN expression does not rescue infection by the prototype IBV strain Mass41. To account for the previous suggestion that fAPN could serve as an IBV receptor, we show that feline cells can be infected with the prototype strain of IBV (Mass 41), but with low susceptibility compared to primary chick kidney cells. We also show that BHK-21 cells are slightly susceptible to certain IBV strains, including Ark99, Ark_DPI, CA99, and Iowa97 (<0.01% efficiency), but this level of infection is not increased by fAPN expression.
We conclude that fAPN is not a functional receptor for IBV, the identity of which is currently under investigation.
The family of Coronaviridae is composed of group 1–3 coronaviruses (CoVs) . These viruses are able to infect human, canine, feline, murine, bovine, porcine, rat, and avian species. The etiological importance and zoonotic characteristics of coronaviruses have received much attention since the discovery of the newly emerged severe acute respiratory syndrome associated coronavirus (SARS-CoV) in 2003 [1, 2]. Coronaviruses have a high frequency of viral genome recombination and polymerase infidelity, which may have contributed to the increase of viral pathogenesis, inter-species transmission, and tissue tropism [3–5]. In the case of SARS-CoV, its ancestral origin remains undetermined, but some evidence suggests that Chinese horseshoe bats may be the natural reservoirs, while Himalayan palm civets harbor and support inter-species transmission to humans [5, 6]. Other examples of extended tissue tropisms can also be found in some group 2 CoVs. It is speculated that the acquisition of hemagglutinin esterase (HE) activity from influenza C virus gives rise to the ability of sialic acid recognition and the extended tissue tropism and pathogenesis for some group 2 CoVs [7–9]. Furthermore, bovine coronavirus (BCV) is thought to have jumped to human hosts, possibly by recombining with influenza C virus, thus giving rise to human coronavirus-OC43 (HCoV-OC43) around 1890 [4, 8].
Receptor interaction between the virus and its host is the first step leading to a successful entry and productive replication. Viruses increase fitness by adapting to environmental pressure through mutation and recombination. In contrast to other families of viruses that utilize a universal receptor to gain entry into host cells, members in the coronavirus family use a variety of cellular proteins and/or co-factors. Group 1 CoVs – including human coronavirus-229E (HCoV-229E), feline infectious peritonitis virus (FIPV), transmissible gastroenteritis virus (TGEV) and canine coronavirus (CCV) – utilize human, feline, porcine, and canine aminopeptidase N (APN) as functional receptors during virus entry [10–13]. The only notable exception is HCoV-NL63, which utilizes angiotensin-converting enzyme 2 (ACE2). In group 2 CoV, mouse hepatitis virus (MHV) of group 2a and SARS-CoV of group 2b independently utilize carcinoembryonic antigen-cell adhesion molecule (CEACAM1) and ACE2 to mediate infection [14, 15]. However, other group 2a CoVs, including HCoV-OC43 and BCoV recognize N-acetyl-9-O-acetylneuraminic acid as a functional receptor . While the cellular receptors for both groups 1 and 2 CoVs have been identified and independently confirmed, group 3 CoV receptors remains undetermined.
The avian CoVs, such as turkey CoV and infectious bronchitis viruses (IBV), have been classified in group 3, with IBV the most extensively studied. Recently, Winter and colleagues suggested that sialic acids are responsible for IBV strain Massachusetts 41 entry . However, group 3 CoVs lack HE as a key viral protein regulating sialic acid binding, and the use of sialic acid would not explain the dependence on chicken cells for infection. Therefore IBV is unlikely to use sialic acids as a functional entry receptor, but rather as a non-specific attachment factor. Heparan sulfate may also serve as an attachment factor for the IBV strain Beaudette (IBV_Bdtt) . IBV_Bdtt is a highly chicken embryo-adapted strain [18, 19], which has an extensive tropism in cell culture and efficiently infects various cell types, including BHK-21 cells [17, 19, 20]. In contrast, clinical isolates and field strains of IBV typically only infect chicken cells.
In the effort to identify the receptor for group 3 CoVs, feline APN (fAPN) was reported to allow entry of the IBV strain Arkansas 99 (IBV_Ark99) . This could therefore be the first indication of a more universal receptor for the CoV family. APN belongs to a family of metalloproteases . It is a type II membrane-bound glycoprotein, and it is expressed on a variety of cell types, including granulocytes, monocytes, and fibroblasts. APN can also be found on the synaptic membranes of the central nervous system neurons, and on epithelial cells in the proximal convoluted tubules, intestinal brush border, and respiratory tract [11, 23]. For coronaviruses in general, there is a cross-species restriction that permits cells of a certain species to be infected only by its own complimentary CoV. However, several studies on FIPV and HCoV-229E, CCV and TGEV have identified fAPN as a universal entry receptor for group 1 coronaviruses [11–13, 23]. The demonstration that fAPN can allow infection by the IBV strain Ark_99 prompted us to examine both prototype and field isolates of IBV and test them for the potential use of fAPN as a receptor.
In this study, we first verified the use of the expressed fAPN as a receptor for FIPV and TGEV by transient and constitutive expression of fAPN in non-permissive BHK-21 cells. We also cultured seven strains of IBV, including Arkansas 99, Arkansas_DPI, California 99, Connecticut 46, Holland 52, Iowa 97, and Massachusetts 41 (designated as Ark99, Ark_DPI, CA99, Conn46, H52, Iowa97, and Mass41) as candidates to test for fAPN utilization by group 3 avian CoVs. Surprisingly, expression of fAPN did not increase viral infection in any of the strains tested. As a consequence, we conclude that fAPN is not a functional receptor during IBV entry. The authentic receptor is still under investigation.
Viruses make use of a variety of receptors to gain entry into their target cells. The ability to recognize sialic acids that are ubiquitously present on the cell surface gives influenza viruses the ability to indiscriminately infect varied tissue or cell types. In contrast, retroviruses require more specialized receptor interactions between viral glycoprotein 120 (gp120) and CD4 as well as other chemokine receptors on the T-helper lymphocytes during invasion . Despite some subtle differences among different species or strains of viruses in the same family, they may still retain a trace of evolutionary similarity in terms of receptor utilization. However, receptor usage appears to have very little consensus among coronaviruses across different groups and species. Coronaviruses seem to be able to devise various strategies to interact with specific target host at the level of receptor binding.
In order to gain a more comprehensive understanding of IBV receptor utilization, we cultured several clinical strains of IBV for this study. Surprisingly, we found that the BHK-21 cells are in fact, weakly permissive to IBV_Ark99, IBV_DPI, IBV_CA99, and IBV_Iowa97 infections. However, fAPN expression on BHK-21 cell surface did not increase viral infectivity for any IBV strain. We therefore conclude that fAPN could not be a functional receptor for IBV entry. It is important to note that one, highly chick-embryo- adapted, IBV strain (IBV Beaudette) gives efficient infection in BHK cells ; combined with the data presented here, that a low level of infection of BHK cells could be obtained with some clinical strains, this suggests that there are no post-entry restrictions to IBV replication and gene expression that might account for a lack of infection of BHK cells.
How do we explain the discrepancy between these data and previous studies that show that fAPN is the receptor for IBV_Ark99 entry? According to our observations, IBV_Ark99 viruses were able to infect BHK-21 cells to a limited degree (<0.01%), although the infection profile was not always consistent among samples. The relevance of this very limited infection of hamster cells is unclear. As a consequence, previous studies may have shown only localized and under-represented populations of cells, which were infected by IBV_Ark99, since there was no further quantification of viral infectivity. In contrast, our present study was performed in a quantitative manner by measuring infection frequency of nearly 106 cells and we provide numerical virus infection data between fAPN-expressing and non-expressing cells.
Various studies have shown several potential receptor candidates for group 3 coronaviruses, which include fAPN, sialic acids, and heparan sulfate [16, 21]. However, there is still little consensus on which of these might be the functional entry receptor in vivo. Sialic acid utilization as a functional entry receptor by IBV remains controversial since IBV generally has a highly restricted species tropism, and does not possess a HE glycoprotein that would normally be responsible for sialic acid destruction and hence efficient virus spread. Unlike influenza viruses that are established to use cell surface sialic acids for entry into a wide variety different cells, IBV is generally considered only to infect cells of chicken origin. The infected chickens often show lesions or lymphocyte infiltration in the ciliated epithelia cells, mucus secreting cells, and sub-epithelial cells along the respiratory tract . IBV also infects lung, ovary, and kidney tissues . In cell culture, IBV_Mass41 infects CKC but not chick embryo fibroblast cells or the chicken DF-1 fibroblast cell line (data not shown), suggesting possible cell type or tissue specificity for IBV infection.
Feline APN has been previously reported to serve as a functional receptor for IBV_Ark99 entry, a conclusion based in part on the ability of the virus to infect feline kidney cells (CRFK, also known as FEK) . We have independently confirmed the ability of feline kidney cells to support IBV_Mass41 infection (Fig. 3A) with relatively high efficiency. As a consequence, cats could potentially serves as an intermediate host during an extension of coronavirus tissue tropism. In the case of SARS-CoV, viral transmission is thought to originate from Chinese horseshoe bats to Himalayan palm civet cats before jumping to humans [5, 6]. The use of a feline receptor for IBV could potentially be another example of coronaviruses using feline cells as an interspecies reservoir to cross species boundaries. The reason why cats may serve as such an intermediate host is unclear, although it may be noteworthy that the endogenous feline coronavirus (FECV) can undergo distinct recombination events within feline cells that result in the emergence of the clinically different virus FIPV . Also of potential relevance is the suggestion that the SARS-CoV S gene may be a mosaic of feline and avian sequences .
We conclude here that IBV cannot utilize fAPN as a functional receptor. It is however important to note that although IBV does not utilize fAPN as an entry receptor, there is still a possibility that chicken APN (cAPN) may serve as the key receptor. Future work will address the potential usage of cAPN, as well as other chicken cell-specific proteins, as IBV receptors.
Viruses and cells
IBV Conn46, Iowa97, H52, and Mass41 were obtained from Dr. Benjamin Lucio-Martinez, Unit of Avian Health, Cornell University, and IBV Ark_DPI was obtained from Dr. Mark W. Jackwood, University of Georgia. IBV Ark99 and CA99 were obtained Dr. Shankar P. Mondal (University of California, Davis). Viruses were propagated in 11 day-old embryonated chicken eggs and purified as described previously . Virus stocks were titered by infection of primary chicken kidney cells, followed by immunofluorescence microcopy with monoclonal anti-S antibodies after 8–12 h of infection. Typically, the highest titer stocks were able to infect between 50% and 70% of the CK cells present, and were used at this concentration for all infections, unless otherwise indicated.
FIPV-1146 and TGEV were obtained from Dr. Edward J. Dubovi, Animal Health Diagnostic Center, Cornell University, and were propagated in CRFK (feline kidney cell) and A72 (canine fibroblast cells) respectively. BHK-21 cells were purchased from ATCC and propagated twice weekly in DMEM, supplemented with 10% fetal bovine serum (FBS) 1% penicillin-streptomycin, and 10 mM HEPES (Cellgro). CRFK and A72 were grown in L:M media. BHKexp.fAPN or and BHKexp.pCiNeo cells were kindly provided by Dr. Kathryn V. Holmes (University of Colorado Health Sciences) and grown in 10% DMEM + 300 μg/ml G418 (Sigma).
Primary chicken kidney cells (CKC) were prepared as follows: in short, SPF White Leghorn Chicks (11–14 days) were sacrificed in a CO2 chamber, and kidneys were removed via aseptic techniques. Kidney cells were washed in 50 ml sterile phosphate buffer saline (PBS) twice via gentle stirring to remove red blood cells. The cells were then treated with 25 ml of trypsin-EDTA (Cellgro) for two minutes to separate large chunks of kidney tissues. Cell supernatant was mixed with 1 ml of FBS and centrifuged at 1,000 rpm for 2 min. Media were removed and kidney cells were resuspended in 25 ml of M20 media. Cell population was determined on a hemocytometer. For plating, 1–1.5 × 106 cell/ml was prepared in M25 media (M20 media with 5% FBS) and incubated in 5% CO2 at 37°C. For infection, 60% confluent cell monolayers were inoculated with virus stocks and incubated for 8, 12, or 24 hours before fixation.
Plasmids and transfections
fAPN/pcDNA3.1D/TOPO and hAPN/pCiNeo plasmid DNAs were provided by Dr. Kathryn V. Holmes (University of Colorado Health Sciences). For standard transfection, 0.6 μg of plasmid DNA were premixed with 2 μl of Lipofectamine 2000 (Invitrogen) in 300 μl of Opti-MEM (Gibco) at room temperature according to manufacture's instructions. Cell monolayers were transfected at 37°C for 24 hours before fixation or virus infection.
Virus infection and immunofluorescence microscopy
Infection and immunofluorescence microscopy were essentially performed as described previously  except with methanol fixation for detecting viral antigens. For fAPN staining, cell monolayers were fixed in 3% paraformaldehyde at room temperature and labeled with monoclonal antibody R-G-4 (provided by Dr. Tsutomu Hohdatsu, Kitasato University, Towada, Aomori, Japan). For viral antigen staining, anti-S1 monoclonal antibody 15:88 was used for IBV Massachusetts 41 and Iowa 97. The anti-S1 monoclonal antibody 13:18 was used for IBV Arkansas_DPI. The anti-M monoclonal antibody 9:19 was used for IBV Arkansas 99, Connecticut 46, California 99, and Holland 52. Monoclonal antibody 17B71 and rabbit polyclonal antibody 367 were provided by Dr. Edward Dubovi, Cornell University, and were used detect FIPV and TGEV infection respectively. The secondary antibodies Alex-fluor 488 goat anti-mouse IgG and Alex-fluor 488 goat anti-rabbit IgG were purchased from Molecular Probes. Cell nuclei were counter-stained with Hoechst 33258 (Molecular Probes). Cells were viewed on a Nikon Eclipse E600 fluorescence microscope. Images were captured with a SPOT RT Monochrome camera and SPOT Advanced v.4.0.9 software and processed with Adobe Photoshop v.7.
We thank (in alphabetical order) Dr. Joel D. Baines, Dr. Edward J. Dubovi, Dr. Tsutomu Hohdatsu, Dr. Kathryn V. Holmes, Dr. Mark W. Jackwood, Dr. Benjamin Lucio-Martinez, and Dr. Shankar P. Mondal for their generous support on providing various reagents possible for this study. We thank A. Damon Ferguson for her skillful technical assistance, and all members of the Whittaker Laboratory for helpful suggestions. This work was supported by grant R03 AI060946 from the National Institutes of Health.
- Spaan WJM, Brian D, Cavanagh D, de Groot RJ, Enjuanes L, Gorbalenya AE, Holmes KV, Masters PS, Rottier PJ, Taguchi F, Talbot P: Virus Taxonomy: Family Coronaviridae. Eighth Report of the International Committee on Taxonomy of Viruses 8th Edition (Edited by: Fauquet CM, Mayo MA, Maniloff J, Desselberg U, Ball LA). London, Elsevier Academic Press 2005, 947–964.
- Webster RG: Wet markets--a continuing source of severe acute respiratory syndrome and influenza? Lancet 2004,363(9404):234–236.View ArticlePubMed
- Makino S, Keck JG, Stohlman SA, Lai MM: High-frequency RNA recombination of murine coronaviruses. J Virol 1986,57(3):729–737.PubMed
- Vijgen L, Keyaerts E, Moes E, Thoelen I, Wollants E, Lemey P, Vandamme AM, Van Ranst M: Complete genomic sequence of human coronavirus OC43: molecular clock analysis suggests a relatively recent zoonotic coronavirus transmission event. J Virol 2005,79(3):1595–1604.View ArticlePubMed
- Li W, Wong SK, Li F, Kuhn JH, Huang IC, Choe H, Farzan M: Animal origins of the severe acute respiratory syndrome coronavirus: insight from ACE2-S-protein interactions. J Virol 2006,80(9):4211–4219.View ArticlePubMed
- Lau SK, Woo PC, Li KS, Huang Y, Tsoi HW, Wong BH, Wong SS, Leung SY, Chan KH, Yuen KY: Severe acute respiratory syndrome coronavirus-like virus in Chinese horseshoe bats. Proc Natl Acad Sci U S A 2005,102(39):14040–14045.View ArticlePubMed
- Luytjes W, Bredenbeek PJ, Noten AF, Horzinek MC, Spaan WJ: Sequence of mouse hepatitis virus A59 mRNA 2: indications for RNA recombination between coronaviruses and influenza C virus. Virology 1988,166(2):415–422.View ArticlePubMed
- Zhang XM, Kousoulas KG, Storz J: The hemagglutinin/esterase gene of human coronavirus strain OC43: phylogenetic relationships to bovine and murine coronaviruses and influenza C virus. Virology 1992,186(1):318–323.View ArticlePubMed
- Schwegmann-Wessels C, Herrler G: Sialic acids as receptor determinants for coronaviruses. Glycoconj J 2006,23(1–2):51–58.View ArticlePubMed
- Yeager CL, Ashmun RA, Williams RK, Cardellichio CB, Shapiro LH, Look AT, Holmes KV: Human aminopeptidase N is a receptor for human coronavirus 229E. Nature 1992,357(6377):420–422.View ArticlePubMed
- Tresnan DB, Levis R, Holmes KV: Feline aminopeptidase N serves as a receptor for feline, canine, porcine, and human coronaviruses in serogroup I. J Virol 1996,70(12):8669–8674.PubMed
- Delmas B, Gelfi J, L'Haridon R, Vogel LK, Sjostrom H, Noren O, Laude H: Aminopeptidase N is a major receptor for the entero-pathogenic coronavirus TGEV. Nature 1992,357(6377):417–420.View ArticlePubMed
- Tresnan DB, Holmes KV: Feline aminopeptidase N is a receptor for all group I coronaviruses. Adv Exp Med Biol 1998, 440:69–75.PubMed
- Nash TC, Buchmeier MJ: Spike glycoprotein-mediated fusion in biliary glycoprotein-independent cell-associated spread of mouse hepatitis virus infection. Virology 1996,223(1):68–78.View ArticlePubMed
- Li W, Moore MJ, Vasilieva N, Sui J, Wong SK, Berne MA, Somasundaran M, Sullivan JL, Luzuriaga K, Greenough TC, Choe H, Farzan M: Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Nature 2003,426(6965):450–454.View ArticlePubMed
- Winter C, Schwegmann-Wessels C, Cavanagh D, Neumann U, Herrler G: Sialic acid is a receptor determinant for infection of cells by avian Infectious bronchitis virus. J Gen Virol 2006,87(Pt 5):1209–1216.View ArticlePubMed
- Madu IG, Chu VC, Lee H, Regan AD, Bauman BE, Whittaker GR: Heparan sulfate is a selective attachment factor for the avian coronavirus IBV Beaudette. Avian Diseases Accepted for publication
- Beaudette FR, Hudson CB: Cultivation of the virus of infectious bronchitis: August 11–14; Columbus, OH. New Jersey Agricultural Experiment Station 1936, 51–60.
- Otsuki K, Noro K, Yamamoto H, Tsubokura M: Studies on avian infectious bronchitis virus (IBV). II. Propagation of IBV in several cultured cells. Arch Virol 1979,60(2):115–122.View ArticlePubMed
- Chu VC, McElroy LJ, Chu V, Bauman BE, Whittaker GR: The avian coronavirus infectious bronchitis virus undergoes direct low-pH-dependent fusion activation during entry into host cells. J Virol 2006,80(7):3180–3188.View ArticlePubMed
- Miguel B, Pharr GT, Wang C: The role of feline aminopeptidase N as a receptor for infectious bronchitis virus. Brief review. Arch Virol 2002,147(11):2047–2056.View ArticlePubMed
- Hooper NM: Families of zinc metalloproteases. FEBS Lett 1994,354(1):1–6.View ArticlePubMed
- Kolb AF, Hegyi A, Siddell SG: Identification of residues critical for the human coronavirus 229E receptor function of human aminopeptidase N. J Gen Virol 1997, 78 ( Pt 11):2795–2802.
- Cavanagh D, Naqi SA: Infectious Bronchitis. Diseases of Poultry (Edited by: Saif SM). Ames, Iowa, Iowa State Press 2003, 101–119.
- Chu VC, McElroy LJ, Bauman BE, Whittaker GR: Fluorescence dequenching assays of coronavirus fusion. Adv Exp Med Biol 2006, 581:241–246.View ArticlePubMed
- Sommerfelt MA: Retrovirus receptors. J Gen Virol 1999, 80 ( Pt 12):3049–3064.
- Vennema H, Poland A, Foley J, Pedersen NC: Feline infectious peritonitis viruses arise by mutation from endemic feline enteric coronaviruses. Virology 1998,243(1):150–157.View ArticlePubMed
- Stavrinides J, Guttman DS: Mosaic evolution of the severe acute respiratory syndrome coronavirus. J Virol 2004,78(1):76–82.View ArticlePubMed
- Sieczkarski SB, Whittaker GR: Influenza virus can enter and infect cells in the absence of clathrin-mediated endocytosis. J Virol 2002,76(20):10455–10464.View ArticlePubMed
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