Replicative homeostasis II: Influence of polymerase fidelity on RNA virus quasispecies biology: Implications for immune recognition, viral autoimmunity and other "virus receptor" diseases
© Sallie. 2005
Received: 31 July 2005
Accepted: 22 August 2005
Published: 22 August 2005
Much of the worlds' population is in active or imminent danger from established infectious pathogens, while sporadic and pandemic infections by these and emerging agents threaten everyone. RNA polymerases (RNApol) generate enormous genetic and consequent antigenic heterogeneity permitting both viruses and cellular pathogens to evade host defences. Thus, RNApol causes more morbidity and premature mortality than any other molecule. The extraordinary genetic heterogeneity defining viral quasispecies results from RNApol infidelity causing rapid cumulative genomic RNA mutation a process that, if uncontrolled, would cause catastrophic loss of sequence integrity and inexorable quasispecies extinction. Selective replication and replicative homeostasis, an epicyclical regulatory mechanism dynamically linking RNApol fidelity and processivity with quasispecies phenotypic diversity, modulating polymerase fidelity and, hence, controlling quasispecies behaviour, prevents this happening and also mediates immune escape. Perhaps more importantly, ineluctable generation of broad phenotypic diversity after viral RNA is translated to protein quasispecies suggests a mechanism of disease that specifically targets, and functionally disrupts, the host cell surface molecules – including hormone, lipid, cell signalling or neurotransmitter receptors – that viruses co-opt for cell entry. This mechanism – "Viral Receptor Disease (VRD)" – may explain so-called "viral autoimmunity", some classical autoimmune disorders and other diseases, including type II diabetes mellitus, and some forms of obesity. Viral receptor disease is a unifying hypothesis that may also explain some diseases with well-established, but multi-factorial and apparently unrelated aetiologies – like coronary artery and other vascular diseases – in addition to diseases like schizophrenia that are poorly understood and lack plausible, coherent, pathogenic explanations.
1.1 Global impact of RNA polymerases
Many of the world's population suffer from acute and chronic viral infection. The two common types of chronic viral hepatitis (CVH), hepatitis B (HBV) and C (HCV) are major causes of death and morbidity; conservative estimates suggest 400 million people are persistently infected with HBV, while HCV may infect a further 200 million. Annually, in excess of two million people will die from cirrhosis or liver cancer caused by CVH, and many more suffer chronic ill health as result. During the 20 years since the human immunodeficiency virus (HIV) was identified, perhaps 40 million people have become infected worldwide and each year about a million die from resulting immunodeficiency and consequent opportunistic infections, particularly tuberculosis, and other complications. Poor countries bear a disproportionate burden of disease caused by these viruses that further exacerbate poverty through pervasive economic disruption and diversion of limited resources to healthcare and disease control. Emerging viral pathogens including West Nile virus (WNV), the SARS coronavirus, endemic viruses like Murray Valley, Japanese, and other encephalitis viruses, Dengue and yellow fever, and seasonal influenza, hepatitis A (HAV) and E (HEV) cause enormous further morbidity and mortality, while pandemic outbreaks of virulent influenza strains remain a constant threat. Together, these viruses probably kill more people every ten days than the Boxing Day Tsunami. RNA viral infections, including Foot and Mouth, Bovine Viral Diarrhea Virus (BVDV) and Hog Cholera Virus (HChV), cause similar devastation of animal populations with enormous economic consequences.
RNA polymerases generate massive genetic variability of RNA viruses and retroviruses that circulate within infected hosts as vast populations of closely related, but genetically distinct, molecules known as quasispecies. After translation, this genetic variability causes near-infinite antigenic heterogeneity, facilitating viral evasion of host defences. Tuberculosis, malaria and other cellular pathogens also express broad cell-surface antigenic heterogeneity, generated by DNA-dependent RNApol. Thus, RNA polymerases probably cause more morbidity and premature mortality in man, and other animals, and greater economic loss, than any other molecule.
1.2 RNA viruses and immune control
Despite a depressing global epidemiology that strongly suggests otherwise, the immune system is thought to "control" viruses. What practical meaning does "immune control" have for the individual? There is no argument for HBV, and other viruses, high affinity antibody, generated by prior vaccination or other exposures and directed against neutralizing epitopes, will prevent HBV infection (excepting vaccine escape mutations [1, 2]), in part by blocking viral ligand interaction with cell receptors, or that most patients exposed to HBV develop neutralizing antibodies (HBsAb), clear HBsAg from serum, and will normalize liver function long term. However, even patients who develop robust immune responses to HBV, defined by high-affinity antiHBsAb and specific antiviral cytotoxic T cell (CTL) responses, will have both "traces of HBV  ... many years after recovery from acute hepatitis"  and transcriptionally active HBV demonstrable in peripheral blood mononuclear cells (PBMCs) . Furthermore, occult HBV is detected in liver tissue of patients with isolated antiHBc (i.e. HBsAg/HBsAb negative)  and in patients with HBsAg-negative hepatocellular carcinoma  suggesting, at least some patients, HBV in may persist irrespective of any immune responses, implying long term latency and low level basal replication may be a survival/reproductive strategy for HBV.
For most patients, acute HCV or HIV infection results in life-long viral persistence. Although many patients develop immunological responses, including specific antibody and CTL reactivity to various viral antigens, these responses have little discernible impact on either HCV or HIV replication that occurs essentially unchecked at rates estimated between 1010 and 1012 virions per day [7, 8], indefinitely, while progressive destruction of liver or immune cells proceeds, commonly resulting in cirrhosis or liver cancer (for HCV) or death from immune deficiency (for HIV). Evidence that prior HCV infection confers no protective immunity against heterologous HCV infection in humans  or chimpanzees  or against either homotypic  or heterotypic  human reinfection, confirmation that active HCV infection persists long after either apparent spontaneous  or treatment-induced  viral clearance, or that vaccines causing specific antiviral B and T cell responses fail to protect against infection in animals , and that antibodies to HCV envelope protein E2 are only detected in animals with persistent infection [16, 17], further undermines the potency of "immune control" and suggests, at least for patients with HCV, the definition of "control" may need to broadened significantly.
Based on observations that stronger specific CD4/CD8 immune responses with T-helper (TH1) cytokine profiles are found more frequently in patients with self limiting viral infections than those who develop chronic viral carriage [18, 19] it is thought ability to mount robust adaptive immune responses predicts viral clearance while failure to do so results in chronic viral carriage . However, detailed and very painstaking studies, albeit in small numbers of chimpanzees  and patients following antiviral therapy , have failed to demonstrate any relationship between T cell responses and viral clearance. Although development of TH1and other immune responses are certainly temporally and, probably, causally related to reduced viral replication and viral clearance the assumed direction of causality (immune response -> reduced viral replication), is not proved by the fact those responses develop, post hoc ergo propter hoc, as comforting a conclusion as it may be to reach.
The first part of this paper explores the impact of RNApol fidelity on quasispecies behaviour, specifically in mediating immune avoidance during acute HCV infection. We suggest the primary event causing reduction in viral replication is inhibition of RNApol processivity by variant viral proteins, specifically envelope and envelope-related proteins. We also suggest that immune responses to viruses are thwarted initially by broad antigenic diversity generated by low RNApol fidelity but develop, when they do, after viral replication falls (because of reduced RNApol processivity) and polymerase fidelity increases – linked events that occur because of replicative homeostasis – thus restricting antigenic diversity sufficiently to permit focused immune recognition. We further suggest immune responses strategically exploit replicative homeostasis to force viruses to reveal critical dominant antigenic epitopes, facilitating progressively more focused immune responses. The second part explores the ineluctable consequence of viral RNA quasispecies: That is, translation of RNAs into protein quasispecies with a spectrum of phenotypes and unpredictable properties, among which may be disruption of the cell surface receptors that viruses co-opt for cell entry. This innate property of viral quasispecies may explain a wide variety of diseases apart from viral autoimmunity.
2. Immunological, viral and biochemical kinetics following acute viral hepatitis
2.1 The replicative kinetic paradox
2.2 Temporal tissue injury (aminotransferase) paradox
Both HBV and HCV are non-cytolytic and viral clearance from hepatocytes, as well as hepatocyte injury, thought to be immune mediated. However, for both HBV and HCV the brisk fall in viral replication following acute infection precedes the peak of transaminase rise by at least two weeks (figure 1). If falling viral replication is due to adaptive immune responses causing hepatocyte lysis the transaminase peak should either precede or be coincident with falling replication. This temporal relationship is also inconsistent with the belief immune factors cause the falling replication seen during acute HCV or HBV, and is analagous to non-cytolytic reductions of viral replication observed for both HBV and lymphocytic choriomeningitis virus (LCV) experimentally, that suggested either [unspecified] antiviral mechanisms are operative [30, 31], or that auto-inhibition of RNApol by viral mechanisms (replicative homeostasis) occurs . However, if other non-cytopathic host anti-viral mechanism(s) are responsible, the kinetic paradox implies their potency falls significantly between points A and B.
2.3 The Hepatitis C "early replication" paradox
Hepatitis C replication kinetics and their relationship to immune responses are well documented [32, 33] but reveal an unexplained paradox. Despite high level viral replication, adaptive cellular immune responses to HCV are completely undetectable for at least 7–10 weeks  after infection, while humoral responses are rarely detected before 12–14 weeks , and in some patients , and some chimpanzees , are never detected at all. An exhaustive and very careful review of the clinical and experimental data relating adaptive immune response and HCV replication kinetics has been published recently . Seeking to rationalize the enigma posed by a complete lack of immune responses to HCV replication of ~106–7 geq/ml at week 6 but [variable] immune responses to replication at ~105 geq/ml after week 14, the authors conclude "..[the data]...appear[s] to be consistent with the interpretation that HBV and HCV are ignored by the adaptive immune system for about 2 months after primary infection" and "[in HCV].. the adaptive response seems to really ignore for several weeks a substantial quantity of virus (at least 106 copies/ml)..". This is certainly an accurate synthesis of an extensive and highly complex literature but does it make any sense?
If adaptive immune responses really ignore high level HCV replication for two months, as suggested, then the following mechanism(s) are implied: a) an accurate mechanism for prompt detection of infection; b) A timing mechanism; c) A trigger mechanism for immune responses independent of any viral factor (given levels of virus are greater before immune recognition than afterwards the trigger for immune response must be either non-viral or falling (!) viraemia); and, as cytomegalovirus (CMV)-specific CD4(+) T cell responses arise within 7 days of CMV infection ; d) A mechanism allowing the immune system to differentiate HCV from CMV and other viruses (and reasons to do so). While possible, this seems unusually inelegant and pointlessly counterproductive, especially as events soon after infection probably determine whether virus is cleared or chronic infection develops. It is much more likely that adaptive cellular or humoral immune responses do not develop in the first 6–7 weeks of HCV infection simply because the virus isn't "seen". Why should HCV replicating at 106–7 geq/ml at week 6 be invisible to the immune system but visible when replicating at 105 geq/ml long term? Dissection of this problem requires explicit analysis of what is being measured and how.
3.1 Hepatitis C: measurement and detection
Assay of HCV RNA and detection of HCV by immune responses measure two quite different things. Quantitation of HCV is typically performed by branch-chain cDNA assay (bDNA) or quantitative PCR (qPCR) using probes or primers complementary to conserved 5'untranslated (5'UTR) HCV RNA sequences. Immune responses to HCV typically "measures" envelope proteins translated from envelope-encoding RNA (EeRNA) sequences and are directed at specific antigenic amino acid sequences and polypeptide conformations, not total viral envelope protein concentrations. While concentrations of 5'UTR RNA will be proportional to EeRNA concentrations in any given sample, they may not be identical for two reasons; i) RNA transcription may prematurely terminate making 5'UTR RNAs relatively more prevalent than EeRNAs and ii) HCV 5'UTR is highly conserved, while EeRNA s are less constrained, making hybridization efficiencies of PCR primers or bDNA probes greater for 5'UTR RNAs than for the population of EeRNAs, causing relative under-estimation of true envelope RNA concentration1. Nonetheless, as 5'UTR HCV RNA concentrations will be proportional to EeRNA concentration, the question remains; why should envelope proteins translated from EeRNA sequences present at concentrations corresponding to ~105 5'UTR geq/ml at 16 weeks be visible immunologically, but envelope proteins derived from EeRNA sequences corresponding to ~106–7 5'UTR geq/ml at 4–6 weeks remain unseen? Quasispecies biology, specifically variable RNApol fidelity, replicative homeostasis, and sequence-specific requirements for both genetic and immunological detection suggest an answer.
4.0 Quasispecies biology: Generation of genomic and phenotypic diversity
The phenotypic consequences of viral quasispecies biology may be more important. Progressive divergence of genomic RNA sequences away from wild-type sequences caused by RNApol infidelity generates a massive population of closely related, but genetically distinct, RNA molecules (figure 3), an effect operative at all scales from each open reading frame (ORF) to whole virus species. A quasispecies of ORF RNAs has but one inevitable outcome; translation of a quasispecies of viral proteins with a vast and highly variable spectrum of phenotypes, some subtly nuanced, others grossly defective. Furthermore, mutations that create new, or obliterate pre-existing, start or stop codons in a significant proportion of RNAs, will cause translation of highly unusual and heterogeneous proteins, particularly during high-level viral replication, a phenomenon that may explain HBeAg. Viral quasispecies cannot, and will not, produce homogeneous proteins with predictable and consistent phenotypic and antigenic properties.
4.1 Quasispecies biology: Frequency distribution of genomic and phenotypic diversity
5.0 Co-evolutionary adaptation
Interactions among species, whether between humming birds and flowering plants, primitive viroids and prokaryotic cells or HCV and man, results in an unremitting process of adaptation and responsive counter-adaptation – in effect, a molecular arms race – for each species just to maintain ecological parity. The price of survival for a species is continual evolution. Survival, for viruses, requires cell entry, a precondition long antedating necessity to evade more complex host defenses, including interferons and other cytokines and adaptive immune responses, while for cells, and complex cellular organisms, cell wall defenses, including receptor polymorphisms, form a principal barrier against viral invasion. Viral survival – effectively meaning RNApol survival – on an evolutionary timescale, as argued previously [28, 44], requires control of mutation and replication rates in a manner adaptively responsive to constantly changing biota and this implies dynamic linkage of RNApol fidelity and processivity with quasispecies phenotypic and antigenic diversity, meaning an autoregulatory linkage – Replicative homeostasis – between RNApol fidelity and processivity and envelope proteins, as argued previously . By definition, evolutionary co-adaptation occurs in response to adaptations in locally prevalent interacting species. Natural selection for beak variation(s) in Darwin's finches occurs as a consequence of concrete survival benefits these variations – mediating, for example, enhanced food harvesting interactions with other variable plant or animal species – confer to individual Galapagos Island birds, rather than any inexorable hypothetical 'improvement' in beak function for finches in general. If a species is widely distributed in space, but population mixing is slow or incomplete, locally prevalent interactions with other species will vary and regional genetic variations will arise and be maintained, hence progressive divergence from the original genotype (speciation) may result. For viruses, and their hosts, genetic variations – reflected in viral genotype and cell surface polymorphisms and resulting disease susceptibilities – would be predicted, and are observed [45–50], to have frequencies that vary geographically.
5.1 Enzymatic Autoregulation
5.2 Co-evolutionary adaptation: Cell-surface polymorphisms
Generation and maintenance of polymorphisms, that is, replacement of existing genes – that, by operational Darwinian definition, have proved their functionality and evolutionary fitness by surviving to reproduce – with variant genes (polymorphisms) of uncertain functionality, fitness or overall compatibility within an organism, is an evolutionary strategy that will only be sustained on a geological timescale if new polymorphisms confer survival benefits to organisms that exceeds the risks and metabolic costs of generating and sustaining those polymorphisms. For primitive cells, lacking functional humoral, cellular or cytokine defense mechanisms, development of cell-surface protein polymorphisms is an obvious adaptive strategy to thwart invasion by primitive viruses. Like other adaptive strategies, cell-surface polymorphisms are strongly selected for, and have been highly conserved over deep time, and are found in all organisms from primitive prokaryotic cells  and thermophilic bacteria  through to plants  as well as mammalian cells, strongly suggesting a critical evolutionary function. The lock and key hypothesis, for which there is very considerable evidence [54–57], first proposed by JBS Haldane , contends polymorphisms arise, and are maintained, as protection against cellular parasitism, particularly by viruses2. While DNA-encoded protein polymorphisms form necessary defenses against viral access, they may not be sufficient; a quasispecies of cells (e.g. the liver) expressing similar and static receptor variations renders those cells vulnerable to sustained attack from any virus that successfully invades any one cell, and further dynamic modification of cell receptors, triggered by viral infection and mediated at the transcriptional level by modulation of DNA dependent RNA polymerase fidelity in nearby uninfected cells, by a mechanism similar to replicative homeostasis would seem possible.
6.0 Problems of Detection
A clear, unambiguous band at the "C" position on a sequencing gel, causes "cytosine" to be assigned to that genetic locus. But does this certitude reflect reality, at least for viral RNA quasispecies? Direct PCR sequencing is an "averaging" procedure revealing the most frequent nucleotide at any particular locus. However, nucleic acids and proteins cannot express 'an average', and discrete quanta of specific nucleotides or amino acids are present at every locus. A typical clinical serum sample, containing 4 × 105 geq/ml HCV and mutating at 10-5 substitutions/base, will contain examples of each possible nucleotide at every locus, but most variations will remain undetected during sequencing or any other method of quasispecies analysis. Analysis of cloned DNA gives cleaner data than PCR sequencing but if 100 clones (and multiple HCV quasispecies clones are highly unlikely to be identical) provides definitive sequence, would we process the 101st to reveal different and, potentially, critical sequence variations? And if we did, how would we recognise its importance? Is important sequence likely to be present at frequencies of < 1%? Infectious virions containing, presumably, full-length functional genome and corresponding wild-type proteins, are often outnumbered by ~6 × 104:1 in serum by defective and non-infectious particles  that presumably do not, suggesting that important genetic sequence and associated phenotype may occasionally be extremely rare. How the immune system recognizes uncommon, nondescript, but important protein sequences in a featureless background of similar molecules is a non-trivial problem for which replicative homeostasis may suggest a solution.
7.0 Replicative Homeostasis
The hepatitis C "early replication" paradox now resolves completely when considered in the context of replicative homeostasis; initial high level HCV replication (due to high RNApol processivity) remains immunologically undetectable for 6–8 weeks, or more, because of low RNApol fidelity causing a broad spectrum of HCV envelope proteins each expressed on cell surfaces at concentrations below the threshold of detection even while viraemia, reflected in concentrations of 5'UTR RNA common to each RNA species, are present at 106–7 geq/ml. As replication progresses, intracellular accumulation of variant viral proteins increase RNApol fidelity but decrease processivity (replicative homeostasis), downregulating HCV replication and reducing viraemia but restricting antigenic diversity and increasing expression of HCV envelope proteins to beyond the threshold of immune detection. Furthermore, the temporal tissue injury (aminotransferase) paradox also resolves in this light: Focussed immune recognition (including cytotoxic T cell responses) doesn't develop until after viral antigenic diversity is restricted by replictive homeostasis the transaminase peak would not be expected until after viral replication falls due to autoinhibition of RNApol processivity. Varying expression of viral proteins by modulating RNApol fidelity to facilitate immune escape would seem a useful evolutionary adaptation that might be retained by more complex organisms, including cellular pathogens like tuberculosis and malaria, to optimize their stability within hosts.
This mechanism of immune avoidance might also explain maternal-foetal tolerance. The human foetus maintains a stable parasitic existence during gestation (and, I expect, to University age and beyond) that is tolerated despite normal maternal immune responsiveness in general and lack of specific tolerance to paternal antigens in particular, a situation made more problematic as expressed foetal antigens are predominantly of paternal origin . While immunological isolation of foetal tissue by the placental trophoblastic layer , and placental display of HLA-G , probably contribute to foetal stability in the face of a potentially robust immune attack, neither mechanism would explain persistence of viable foetal nucleated red blood cells within the maternal circulation  in quantities sufficient to permit clinical prenatal diagnosis . Is it possible foetal tolerance is mediated by regulating the fidelity of foetal DNA dependent RNA transcriptases to ensure any cell-surface antigens are expressed heterogeneously and at levels below the threshold of maternal immune responsiveness?
For many classical autoimmune disorders, including primary biliary cirrhosis , multiple sclerosis, and rheumatoid arthritis, convincing epidemiological evidence , including cases clustering [61, 62], strongly suggests these diseases are triggered by infectious agents in genetically predisposed individuals. In others, such as diabetes mellitus, tantalizing epidemiological , clinical  and laboratory  evidence has implicated enteroviruses, but has suggested viral-triggered autoimmune processes, rather than cytolytic destruction of pancreatic beta-cells . Similar circumstantial evidence exists for myocarditis, demyelinating diseases, myositis and other post infectious inflammatory disorders. When MacFarlane Burnet wrote autoimmunity arises from "inability to distinguish self from non-self" HBV, HCV, HIV and other viruses, now established to cause diseases with clear autoimmune features were unknown. Viral infections, particularly hepatitis C – and its treatment with interferon – are associated with many varied autoimmune phenomena , and thyroid disease [68–70], diabetes mellitus [71, 72], membranous, membranoproliferative and cryoglobulinemic glomerulonephritis, vasculitis and peripheral neuropathy , and autoimmune gastritis  are all very well documented, although the mechanism(s) are unknown and causality is certain. Classical serological markers of autoimmunity, including rheumatoid factor, antinuclear antibodies (ANA), anticardiolipin, antithyroid, anti-liver/kidney/microsomal antibodies (anti-LKM), as well as HCV/anti-HCV immune complex formation and mixed essential cryoglobulinemia are common accompaniments of chronic HCV infection , raising the obvious question of whether all "autoimmunity" has a viral basis. Indeed, Zinkernagel's pragmatic and subtly anticipatory; "If we know the infection, we call the disease immunopathologically mediated; if we do not recognize or know it, we call the disease autoimmune " fully reflects recent explosive growth of information and the deeper questions this information poses.
10.0 Virus receptor disease
RNA virus quasispecies biology, specifically the generation of RNA quasispecies by RNApol, and translation of these immensely variable RNAs into protein quasispecies, suggests an immediate solution to the problem of viral autoimmunity and, by extension, to autoimmunity in general, as well as suggesting a unifying hypothesis to explain other diseases known to have multi-factorial aetiologies that include inflammatory components – such as coronary artery disease – in addition to other diseases – including schizophrenia and some forms of depression – that currently lack rational and coherent pathogenic explanations.
Viruses are known to co-opt cell surface molecules, including lectins, hormone receptors and cell signaling molecules, to access cells. Receptors, and other cell surface molecules, identified as "viral receptors"or to specifically interact with viral proteins include prostaglandins, catecholamines and acetylcholine receptors , serotonergic neurotransmitters (5HT) , endothelial cell glycoproteins , insulin-like growth factor (IGF-IR) and its major signaling molecules insulin receptor substrates IRS-1  and IRS-2 , epidermal growth factor (EGF) , neurotrophin receptor , thyroid hormone receptor TRalpha1 , an immunoglobulin protein superfamily , low density lipoprotein (LDL) receptors [85, 86], transferrin receptor (TfR) , asialoglycoprotein receptor (ASGP-R) [88, 89], and angiotensin-converting enzyme 2 , to cite biologically diverse examples. Of necessity, some receptor affinity studies have used cloned viral protein ligands, an artificial situation that cannot approach the phenotypic complexity of RNA viral protein quasispecies. Nonetheless, variable virus receptor affinities [91, 92], evolutionary adaptation of receptor affinity , emergence of escape variants with altered receptor affinities , temporal alteration of receptor usage  and capacity to exploit alternative entry pathways  have all been confirmed, suggesting viruses are capable of generating highly plastic ligands with very broad receptor affinities.
If a virus co-opts a receptor for cell entry, then wild-type envelope (consensus sequence) epitopes, coded for by wild-type RNA sequences, will probably form the common viral ligand. However, any viral RNA quasispecies also contain a vast spectrum of RNAs derived from, and similar to, envelope open reading frame (ORF) consensus sequence, but variant from it. As the envelope ORF quasispecies sequences progressively diverge from wild-type, the quasispecies of envelope proteins translated from these variant ORFs will also, and inexorably, diverge in sequence, structure and biological function from wild-type envelope sequence proteins. Some of these envelope proteins will be functionally identical, but others, and probably the vast majority, will range from subtly different to grossly abnormal, either due to major differences of sequence and/or chemical or steric amino acid incompatibility, or because of premature introduction of stop codons. Even minor amino acid differences, as sickle cell anaemia illustrates, and has been confirmed specifically for viral receptor usage [96, 97], may catastrophically alter a proteins' function with respect to co-opted viral receptors, with some having no binding affinity, while others will bind strongly and act as agonists, antagonists or competitive inhibitors of normal receptor function. Variant and defective viruses, and their polypeptides, will be in vast molar excess compared to wild-type  but will exhibit similarly high antigenic variability, permitting escape from immune and other scavenger mechanisms. As many variant viral polypeptides will bind tightly to "self" receptors, but contain immunogenic non-self motifs, a polymorphic (because variant viral proteins will themselves be highly polymorphic due to the quasispecies process) immune response, apparently directed against "self" antigens, but actually targeting virus protein-receptor complexes virtually indistinguishable from normal cell receptors, will result causing apparent 'autoimmune' tissue damage.
This mechanism suggests an explanation for common autoimmune phenomena. If a virus enters cells because wild-type envelope motifs interact with insulin, insulin receptor substrate [79, 80], TSH or related molecules , or acetylcholine  receptors, many variant envelope polypeptides, generated by envelope ORF quasispecies RNAs, would have similar receptor binding affinity, but may effectively disrupt receptor function, predictably causing impaired glucose tolerance or diabetes mellitus, thyroid dysfunction, or myasthenia gravis with secondary resistance to, and elevation of, the normal hormone ligand (insulin, TSH etc.). The expected consequences disruption of receptor function by variant viral proteins might explain many common biochemical pathologies; For example, what effect would chronic blockade of parathyroid (PTH) receptors by viral proteins have on PTH levels, the parathyroid glands, or bone?
Leptin is a 16Kda protein hormone secreted by adipocytes and carried across the blood-brain barrier by a rate-limiting transporter to act on hypothalamic receptors  where, among other functions, it regulates thyrotropin-releasing hormone (trh) genes and upregulates alpha-melanocyte-stimulating hormone and other anorexigenic neuropeptides  important to appetite-regulation and energy balance . Leptin also regulates a broad spectrum of other processes and behaviours including thermogenesis, blood pressure and immune function. s=Serum leptin concentrations and leptin resistance, are independent markers of obesity, weight gain, systemic hypertension , diabetes mellitus , obstructive sleep apnoea  and myocardial infarction , while polymorphisms of the leptin gene are associated with insulin resistance  and long-term risk of developing diabetes mellitus . Predictably, variant envelope proteins generated by envelope ORF RNA quasispecies from viruses utilizing leptin receptors for cell access would have similar receptor affinity, but exhibit non-physiological leptin antagonist or agonist properties, thus disrupting leptin receptor function, altering energy regulation, and causing either excess caloric intake unrestrained by satiety responses, or inappropriate satiety signals with pathologically reduced caloric intake. As clear evidence exists for viral disruption of leptin function  and virus-associated weight gain in humans  and monkeys , is it possible the global epidemics of type II diabetes mellitus, insulin resistance, hyperlipidaemia and obesity now prevalent [109–116], are just that; epidemics fundamentally caused by viruses that co-opt insulin or leptin or other associated receptors for cell access and generate protein quasispecies that disrupt receptor function? Could it also be that ethnically based epidemics of obesity, diabetes mellitus, hypertension and reno-vascular disease (the 'metabolic syndrome'), as seen in PIMA Indians, Nauruans and Australian Aborigines  have developed not primarily because of exposure to "Western" foods and lifestyles – that, after all, are all-pervasive without necessarily having so dramatic an effect on other groups – but because of chronic or recurrent exposure to viruses, or genotypes of viruses to which their particular repertoire of receptor polymorphisms confer no protection? Or that anorexia nervosa develops, in some patients, when variant viral proteins with aberrant leptin-agonist function arise during the course of viral infection, as the temporal relationship between infection and disease onset, very clearly documented in one study , suggests.
Cardiovascular disease, the leading cause of premature death and disability in most western countries, has a well-established multi-factorial basis involving a complex interplay between genetic predisposition, environmental and personal risk factors – including systemic hypertension, diabetes mellitus, hyperlipidaemia, obesity and cigarette smoking – and more recently recognized mechanisms, including endothelial dysfunction , vascular inflammation  and leptin levels . Systemic hypertension, diabetes mellitus and hyperlipidaemia have long-established, but complex, patterns of inheritance, a situation further compounded by evidence receptor polymorphisms – including those of angiotensin II type 1 receptor , IRS-1 gene  and low density lipoprotein receptor (LDLR)  – both confer disease susceptibility and have regionally variable prevalences [123, 124].
Human immunodeficiency virus HIV-associated dementia (HIVD) occurs in 15% of HIV-infected adult patients, and as a major cause of dementia in the young represents "proof of principle" of virus-caused dementia, raising the possibility other forms of virus related dementia exist. Although highly active antiretroviral therapy (HAART) has reduced the incidence of HIV-D by 40–50% , it remains a major cause of morbidity and the pathogenesis poorly understood. Direct cytopathic effects of HIV or other viruses are unlikely, while active replication of virus, high-level viral protein expression , and increased viral envelope sequence-diversity in blood and brain  are all important, clearly indicating viral proteins are pathogenically important. The clinical features of HIVD, including psychomotor slowing, apathy, and altered gait and posture, strongly suggest a subcortical dementia with involvement of the basal ganglia and striatal dopamine receptor pathways. Schizophrenia, depression and bipolar affective disorder, and anorexia nervosa are highly prevalent, chronic conditions of unknown aetiology that cause enormous morbidity and generate significant health care costs. Each of these disorders have well documented, albeit regionally variable, associations with receptor – including dopamine – polymorphisms [124, 139–143], as well as epidemiological evidence that viral infections are aetiologically important, either directly or as precipitating events [117, 144–147], although other sero-epidemiological studies  and work directly seeking viral nucleic acids in patients with schizophrenia have proved negative . If a virus, or viruses, use dopamine, acetylcholine , neurotrophin , serotonergic (5-HT), or other neuro-transmitter receptors to access cells (and, given RNA virus quasispecies biology, it would be surprising if some didn't), then the RNA quasispecies will generate a quasispecies of variant polypeptides potentially reactive to these receptors. While it is difficult to imagine what effect perfusing a functional human brain with a solution of antigenic, inflammatory polypeptides that bind to, and are variably disruptive of, critical neurotransmitter receptor function, might have on cognition, perception, behaviour, attention span, abstract thought, fine motor or emotional control, it is unlikely to be beneficial. In this context, the well-documented cognitive abnormalities – unrelated to depression – found in patients with early HCV and HIV infection [150–152] are unsurprising.
12.0 Virus Receptor Disease: Conclusions
Virus receptor disease (VRD) is quite distinct from either immune complex deposition disease due to deposition of macromolecules in tight vascular arcades, or from disease related to altered cell tropisms and is also completely independent of the primary site of viral replication; both non-inflammatory receptor blockade and immune-mediated inflammation directed at viral protein-receptor complexes could cause pathology of tissues non-permissive for and remote from the primary site(s) of viral replication with "autoimmune" damage to the liver, pancreas, brain, skin or lungs arising, for example, from chronic small intestinal virus infection. Viral quasispecies biology predicts VRD will have other characteristics. First, due to replicative homeostasis, the ratio of wild type to variant viral proteins of the quasispecies will both fluctuate with time and will alter dramatically after initial infection; if wild-type proteins are dominantly agonist in function with respect to their receptor, variant proteins, most likely, will predominantly exhibit antagonist function (and vice versa). Furthermore, the net effect of viral proteins (because of viral autoregulation) will fluctuate initially between receptor agonist and antagonist function, before becoming predominantly antagonistic, thus providing a possible explanation for transient thyrotoxicosis during early thyroiditis (before hypothyroidism supervenes), for hypoglycaemia seen during early insulin-receptor antibody-mediated insulin resistance , and for the contradictory functions ascribed to HIV nef . A corollary of fluctuating phenotypic dominance of viral protein quasispecies is that receptor affinity of these proteins will also fluctuate, and any resulting inflammation may vary in both intensity and anatomical distribution over time. Second, because viruses utilize alternate receptors for cell access, apparently homogeneous disease processes could result from multiple different viruses. Similarly, because virus quasispecies produce a broad spectrum of protein phenotypes, and the receptor polymorphisms permissive for cell entry for specific viruses will be variably distributed in host populations, pathology of widely variable tissues in different individuals could result from the same virus. Third, as evolutionary co-adaptation results in progressive genetic co-divergence of interacting species, the receptor polymorphisms predisposing to (or protecting against) infection by any particular virus, and resulting VRD, and the common viruses causing them, would be predicted to vary geographically, an expectation multiply confirmed for disease associated polymorphisms. As a corollary this suggests individuals migrating from regions where hosts and virus strains are stably co-adaptated to other areas, where different viruses are prevalent, might experience increased rates of VRD – beaks optimally adapted for finch survival on the Galapagos may be a liability elsewhere – a prediction again amply confirmed [155–157];.
Finally, if immune mechanisms are unable to clear RNA viruses like HCV and do not cause the reduced viral replication seen during acute infection, are they any more likely to be effective against other RNA viruses? Is it possible that self-limiting infections like influenza and SARS also autoregulate their replication, and, like HCV or HBV, become partially dormant, yet remain transcriptionally active, in the face of an active and powerful immune response? PCR amplification of influenza RNA from convalescent samples makes this readily testable, while the documented relationship of influenza to myocardial infarction  and juvenile rheumatoid arthritis  makes the question important. If confirmed, the well-documented seasonality of some depressive illnesses  and schizophrenia,  and increased rates of schizophrenia during influenza epidemics , and the increased incidence of both depression  and schizophrenia [144, 145] following in-utero exposure to influenza may be more rationally explained.
I thank my wife Sophie J Coleman, and sons Matt and Tim, for everything important, my parents Dick and Janet for extraordinary opportunity, and some great physician-teachers – that most noble vocation – of the University of Western Australia Medical School; Professors Mike McCall, Dick Joske, Bill Reed, Bill Musk, Peter Pullan, Michael Quinlan, Dick Lefroy and Ted Haywood. Special thanks to Karl Ruckriegel for turning back-of-envelope sketches into first-class graphics. Any remaining lack of clarity is my fault.
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