Kobuvirus belongs to the family Picornaviridae, a small and non-enveloped virus with a single stranded, positive-sense genomic RNA. The genus Kobuvirus consists of three species, Aichivirus A (formerly Aichi virus), Aichivirus B (formerly Bovine kobuvirus) and Aichivirus C (aka porcine kobuvirus). Kobuviruses have a wide range of host species including humans
 and goats
PKV was first identified from stool specimens of clinically healthy and diarrhea domestic pigs in 2007 in Hungary
. In the past several years PKV has been identified in China
 and Brazil
During the past three years, China has suffered from severe porcine diarrhea and serious economic loss. Apart from the three main viruses, Porcine Epidemic Diarrhea Virus (PEDV), Transmissible Gastroenteritis Virus (TGEV) and A group Porcine Rotavirus (PoRV), which causing porcine diarrhea, PKV was identified from the diarrhea pigs, separately existed or mixed infected with the three viruses. Recent studies indicated that PKV was involved in swine diarrhea and may act as a special part in gastroenteritis pathogenesis
. Early detection and diagnosis can serve to control the spread of PKV and decrease economic loss.
Most studies investigating the distribution and prevalence of PKV employed reverse transcription-polymerase chain reaction (RT-PCR) method to amplify the conserved region, which targeting the 3D region of PKV genome
[18, 19]. At present, no more detection methods were reported except RT-PCR method. Sequence analysis indicated the 3D region is the most conservative, which suggests that the 3D region could be selected as the target sequence for loop-mediated isothermal amplification detection to investigate the distribution and prevalence of PKV.
As PCR requires an expensive thermal cycler and operator skill, which are limited to the field, a novel technique, loop-mediated isothermal amplification (LAMP) was developed by Notomi et al.
 to overcome the difficulties of PCR-based techniques. RT-LAMP assays are more sensitive than conventional gel-based RT-PCR assays, fast and easy to perform since they require only a simple incubator, such as a heating block or a water bath to provide a constant temperature for the reaction
. This technique just requires only 30 to 60 minutes and can be performed at a single temperature ranging from 60°C to 65°C. Therefore, this study was to develop a fast diagnostic method based on the LAMP reaction for rapid detection of PKV nucleic acid in fecal samples.