Open Access

Genetic characterization of type 2a canine parvoviruses from Taiwan reveals the emergence of an Ile324 mutation in VP2

  • Chao-Nan Lin1, 2Email author,
  • Chi-Hsien Chien1, 2,
  • Ming-Tang Chiou1,
  • Ling-Ling Chueh3,
  • Meng-Yu Hung1 and
  • Han-Siang Hsu1, 4
Virology Journal201411:39

DOI: 10.1186/1743-422X-11-39

Received: 10 December 2013

Accepted: 19 February 2014

Published: 25 February 2014

Abstract

Background

Canine parvovirus 2 (CPV 2) is a major infectious cause of mortality in puppies. The characteristic symptom of CPV 2 disease is intestinal hemorrhage with severe bloody diarrhea. Soon after CPV was first recognized in the late 1970s, the original virus, CPV 2, was replaced in the canine population by strains carrying minor antigenic variants (termed 2a, 2b, and 2c) of the VP2 gene that could be distinguished using monoclonal antibodies and molecular analyses. Here, we provide an updated molecular characterization of the CPV 2 circulating in Taiwan.

Methods

In this study, 28 isolates of CPV 2 from 144 dogs with suspected CPV infection were obtained from northern, central, and southern Taiwan from 2008 to 2012 and screened by PCR. The 28 isolates were sequenced, and a phylogenetic analysis of the VP2 gene was performed.

Results

Of the 28 Taiwanese CPV 2 isolates, 15 were identified as new CPV 2a, and 13 were identified as new CPV 2b. Compared to the reference CPV 2a, all 15 of the CPV 2a sequences collected in this study contain an Ile324 mutation caused by a TAT to ATT mutation at nucleotides 970–972 of the VP2 gene.

Conclusion

Our VP2 sequence data revealed that both types are currently prevalent CPV 2 field strains circulating in Taiwan, and a unique Ile324 VP2 mutation was found in our Taiwanese CPV 2a isolates and recent Asian isolates. CPV 2c was not observed in this study.

Keywords

Canine parvovirus Genotype VP2 Sequence analysis

Background

Canine parvovirus (CPV) enteritis is characterized by intestinal hemorrhage with severe bloody diarrhea [1]. The causative agent, CPV 2, was first identified in the late 1970s [2]. CPV is a non-enveloped, linear, single-stranded DNA virus with a genome of approximately 5 kb, and it belongs to the genus Parvovirus, together with feline panleukopenia virus (FPV), mink enteritis virus, raccoon parvovirus, and porcine parvovirus [3]. Indeed, CPV 2 is believed to have originated from FPV [4, 5], and various hypotheses for how this may have occurred have been suggested, including direct mutation from FPV and contact between cats and dogs kept as companion animals within the same home [5].

An antigenic variant, CPV 2a, developed within a few years after the emergence of CPV 2 [6, 7], and another CPV 2 variant, CPV 2b, began appearing in the canine population in the mid-1980s [8]. In 2000, a new antigenic variant, CPV 2c, was first detected in Italy [9]. New antigenic types of CPV 2 have been found in epidemics worldwide and are replacing the original CPV 2. The antigenic variant CPV 2a shows the following substitutions within the VP2 protein: Met87Leu, Ile101Thr, Ala300Gly, and Asp305Tyr. Furthermore, CPV 2b has been confirmed to contain an additional substitution, Asn426Asp [10, 11]. These two variants further evolved into new 2a and 2b types, with substitutions of Ser297Ala, during the 1990s [12]. Antigenic variant CPV 2c was identified with a substitution Asp426Glu [9]. Different antigenic variants of CPV 2 predominate in different countries [1242].

A retrospective analysis has revealed that the oldest CPV 2c strain was identified in 1996 in Germany [18], and the results from European epidemiological surveys show that CPV 2c is now predominant in Italy, Germany, and Spain and is also widely co-distributed with CPV 2a or CPV 2b in Portugal, France, and Belgium [18, 4347]. Outside of Europe, CPV 2a and 2b isolates are common in the United States [19, 41], whereas CPV 2c is more widespread in Uruguay [20, 32], Brazil [33], and Argentina [30, 48]. Surprisingly, either CPV 2a or CPV 2b is the predominant variant in Asian countries [12, 13, 15, 21, 22, 2426, 28, 29],[35, 37, 38] and Australia [42], though a few CPV 2c strains have been isolated in India [26]. Interestingly, a new amino acid substitution, Tyr324Ile, was identified in Korea [21, 24], China [29], Thailand [28], Uruguay [32], Japan [38], Taiwan [35], and India [37, 49].

In Taiwan, as in other Asian countries, variants CPV 2a and 2b have predominated since the first outbreak [13, 15, 35]. However, few recent studies have included a genetic analysis of Taiwanese CPV 2 strains. Therefore, the aim of this study was to clarify the evolution of CPV 2 isolated from northern, central, and southern Taiwan during the 2008–2012 period.

Results

PCR amplification and genotype analysis

A total of 28 cases from 144 dogs showed positive results for CPV 2. All of the CPV 2 isolates were clearly separated into two genotypes (Figure 1). Of the 28 Taiwanese CPV 2 strains, 15 were identified as new CPV 2a, and 13 were identified as new CPV 2b. Of the 15 new CPV 2a isolates, 3 (20%), 2 (13.3%), and 10 (66.7%) isolates were collected from northern, central, and southern Taiwan, respectively (Table 1). Of the 13 new CPV 2b isolates, 3 (23.1%), 10 (76.9%), and 0 (0%) isolates were collected from northern, central, and southern Taiwan, respectively (Table 1). Taken together, our results show that both types are currently prevalent CPV 2 field strains circulating in Taiwan.
https://static-content.springer.com/image/art%3A10.1186%2F1743-422X-11-39/MediaObjects/12985_2013_Article_2380_Fig1_HTML.jpg
Figure 1

Phylogenetic relationships based on the partial VP2 gene of CPV 2 between Taiwanese isolates and reference strains. The analysis was performed employing the maximum likelihood method based on 1,000 replicates using MEGA 5 software. N: Northern Taiwan, C: Central Taiwan, S, Southern Taiwan. Light grey underlay: CPV 2 isolates in the present study. Italic: the Ile324 mutation in VP2.

Table 1

The genotypes of 28 canine parvovirus type 2 isolates collected from Taiwanese dogs

Strain*

Sampling time

County (Region)

Age**

Vaccination

Genotype

Accession number

176R

2008/JUL

Taipei (North)

NA

NA

2b

KF906327

177R

2008/OCT

Taipei (North)

NA

NA

2b

KF906328

178R

2009/JAN

Taipei (North)

6Y

Yes

2a

KF906329

180BR

2011/DEC

Taipei (North)

NA

NA

2b

KF906330

181BR

2012/JAN

Taipei (North)

4.5 M

No

2a

KF906331

182BR

2012/JAN

Taipei (North)

4.5 M

No

2a

KF906332

077R

2012/JUL

Taichung (Central)

6Y

Yes

2b

KF906333

098R

2012/OCT

Taichung (Central)

3 M

Yes

2b

KF906334

099R

2012/OCT

Taichung (Central)

1Y

Yes

2b

KF906335

100R

2012/OCT

Taichung (Central)

1Y

No

2b

KF906336

102R

2012/NOV

Taichung (Central)

3 M

NA

2b

KF906337

103R

2012/NOV

Taichung (Central)

1Y

No

2b

KF906338

104R

2012/NOV

Taichung (Central)

1Y

No

2b

KF906339

105R

2012/NOV

Taichung (Central)

4 M

No

2b

KF906340

106R

2012/NOV

Taichung (Central)

10 M

No

2b

KF906341

107R

2012/NOV

Taichung (Central)

1Y

No

2b

KF906342

117R

2012/DEC

Taichung (Central)

6 M

No

2a

KF906343

118R

2012/DEC

Taichung (Central)

6 M

No

2a

KF906344

011BR

2012/MAR

Pingtung (South)

2 M

No

2a

KF906345

017R

2012/MAR

Tainan (South)

2 M

Yes

2a

KF906346

028B

2012/MAY

Pingtung (South)

1Y

Yes

2a

KF906347

030BR

2012/MAY

Pingtung (South)

3 M

Yes

2a

KF906348

031B

2012/MAY

Pingtung (South)

4 M

No

2a

KF906349

033B

2012/MAY

Pingtung (South)

1 M

No

2a

KF906350

039B

2012/MAY

Pingtung (South)

3 M

No

2a

KF906351

040B

2012/MAY

Pingtung (South)

3 M

No

2a

KF906352

041BR

2012/MAY

Pingtung (South)

3 M

No

2a

KF906353

106BR

2012/NOV

Pingtung (South)

1Y

Yes

2a

KF906354

*The letters in each name indicate the specimen source: B, whole blood; R, rectal swab.

**Age of the dog at presentation, Y = years; M = months; NA: not available.

DNA sequence analysis

The partial VP2 nucleotide sequences were analyzed using DNASTAR software, revealing 99.4-100, 99.7-100, and 99–99.4% homology within the local CPV 2a isolates, within the local CPV 2b isolates, and between the local CPV 2a and 2b isolates, respectively (Table 2). In comparison to the low nucleotide sequence similarity between reference Taiwanese CPV 2a and our CPV 2a (99.4-99.5%), the homology levels between our analyzed CPV 2a isolates (99.7-99.8%) and Korean CPV 2 K026 (EU009204) appeared to be much higher (data not shown).
Table 2

Sequence homology of local new CPV 2a and 2b isolates and reference strains

Strains

Genotypes

New CPV 2a

New CPV 2b

 

AY742953 (CPV 2a-435)

AB054213 (Taiwan 9)

Taiwanese CPV 2a*

AY742955 (CPV 2b-436)

AY869724 (Taichung)

Taiwanese CPV 2b**

AY742953 (CPV 2a-435)

100.0

99.8

99.2-99.4

99.7

99.7

99.5

AB054213 (Taiwan 9)

 

100.0

99.4-99.5

99.8

99.8

99.7

Taiwanese CPV 2a*

  

99.4-100.0

99.2-99.4

99.2-99.4

99.0-99.4

AY742955 (CPV 2b-436)

   

100.0

100.0

99.8

AY869724 (Taichung)

    

100.0

99.8

Taiwanese CPV 2b**

     

99.7-100.0

*Accession numbers: KF906329, KF906331, KF906332, KF906343, KF906344, KF906345, KF906346, KF906347, KF906348, KF906349, KF906350, KF906351, KF906352, KF906353, KF906354.

**Accession numbers: KF906327, KF906328, KF906330, KF906333, KF906334, KF906335, KF906336, KF906337, KF906338, KF906339, KF906340, KF906341, KF906342.

Amino acid sequence analysis

Amino acid comparisons among the 28 isolates and the 12 reference strains revealed a major region of great diversity at amino acids 297–324 (Figure 2). We also examined the 2 amino acids (positions 297 and 426) within this partial VP2 gene identified by Ohshima [12] et al. and Buonavoglia et al. [9] that were proposed to distinguish CPV 2a/2b from new CPV 2a/2b and CPV 2a/2b/2c, respectively. Our alignment revealed that all of our new CPV 2a and 2b strains contained Ala297 (Figure 2). The amino acid Glu426, which is unique to strain CPV 2c, was no observed in any strain in this study. Four nonsynonymous mutations were observed in our CPV 2a and/or 2b strains (TGT to TGC, CTA to CTG, AAT to AAC and CAA to CAG). One unique amino acid substitution was found in the all of our CPV 2a isolates (Tyr324Ile), caused by the mutation of TAT to ATT at nucleotide positions 970–972 of the VP2 gene. Our CPV 2a isolates were more closely related to the Ile324 isolates from other countries than to the prototype Taiwanese CPV 2a Taiwan 9 (Figure 1).
https://static-content.springer.com/image/art%3A10.1186%2F1743-422X-11-39/MediaObjects/12985_2013_Article_2380_Fig2_HTML.jpg
Figure 2

Multiple amino acid sequence alignment of the partial VP2 gene of CPV 2. Only those amino acid sequences differing from the FPV sequence are shown. The heterogenic region is boxed.

Discussion

CPV 2, which causes intestinal hemorrhage with severe bloody diarrhea in dogs, is distributed worldwide, and genetic variation among CPV 2 isolates could be used to further classify the viruses into four genotypes (2, 2a, 2b, and 2c) that differ in their amino acid sequence and VP2 gene phylogenetic relationships [10]. CPV 2a and 2b are the predominant variants in Asia [12, 13, 15, 21, 22, 2426, 28, 29],[35, 37, 38], whereas CPV 2c is recently distributed on several continents, including Europe (Italy, Germany, Spain, Portugal, France, Belgium, UK, Greece, and Bulgaria) [18, 43], Africa (Tunisia) [23], North America (USA) [19, 41], South America (Brazil [33], Uruguay [20, 32], Argentina [30, 48], and Ecuador [34]), and Asia (India) [26]. The first case of CPV 2c was identified in 1996 in Germany [18]. Although a few CPV 2c cases have been reported in Asia (India) [26], no CPV 2c was observed in the present study.

This is the first study to investigate the genotype prevalence of CPV 2 in northern, central, and southern Taiwan in recent years. Our results indicate that both types are currently prevalent CPV 2 field strains circulating in Taiwan. Surprisingly, all of the CPV 2a isolates contain a unique amino acid substitution (Tyr324Ile). Our review of the GenBank database and sequence analyses showed that this Ile324 variant of CPV 2a is also found in Korea [21, 24], China [29], Thailand [28], Uruguay [32], Japan [38], Taiwan [35], and India [37, 49]. Interestingly, with the exception of Uruguay, this Ile324 CPV 2a variant is only distributed in Asian countries, and our data revealed that this variant was first found in Taiwan in 2009. We believe that the Ile324 CPV 2a variant emerged a few years ago. Taken together, our results suggest that the Ile324 CPV 2a variant may be present due to i) importation from abroad or ii) evolution from existing CPV 2a genotypes. An ongoing investigation is aimed at distinguishing between these possibilities.

VP2 encodes a viral capsid protein that is the major structural protein of CPV 2 and is involved in the host immune response [50]. Therefore, a small number of mutations may result in increased pathogenicity [34], and the effectiveness of commercial vaccines against the Ile324 variant of CPV 2a requires further evaluation. Among all carnivore parvoviruses, residue 324 of VP2 is subject to positive selection [51] and is adjacent to a residue (amino acid 323) known to be involved in host range and tropism via canine transferrin receptor binding [52]. The mutation of CPV 2 residue 323 may influence interactions between residues in neighboring loops of either the same VP2 molecule or the threefold-related VP2, greatly decreasing replication in canine cells [53]. Although the function of residue 324 remains to be elucidated, using SYBR Green-based real-time PCR, our previous study detected the viral shedding of the Ile324 CPV 2 variant for up to 63 days in naturally infected dogs [54]. The pathogenesis of this new variant requires further investigation.

The first CPV 2 infection in Taiwan was recorded in 1980 and was attributed to CPV 2, which was later replaced by CPV 2a and 2b [13]. Previous studies have shown that the predominant genotypes of CPV 2 in northern, central, and southern Taiwan were CPV 2a [13], CPV 2b [15], and CPV 2a [35], respectively, in agreement with our results. Thus, both genotypes (CPV 2a and CPV 2b) constitute the prevalent CPV 2 field strains circulating in Taiwan in the last two decades. However, CPV 2 is constantly mutating, leading to the evolution of novel CPV 2 variants. For example, the CPV 2a Ile324 variant and CPV 2c Glu426 variant have emerged worldwide. Additional CPV 2 cases need to be investigated using continuous surveillance and sequence analysis.

Conclusion

Our VP2 sequence data revealed that both types are currently prevalent CPV 2 field strains circulating in Taiwan, and a unique Ile324 VP2 mutation was found in our Taiwanese CPV 2a isolates and in recent Asian isolates. CPV 2c was not observed in this study.

Methods

Specimen collection and DNA extraction

Clinical samples (whole blood and/or rectal swab) were collected from 144 dogs in northern, central, and southern Taiwan between 2008 and 2012. These samples were mainly acquired from dogs with diarrhea and/or bloody diarrhea. The year of sampling, age, clinical history, and CPV types of the sampled dogs are summarized in Table 1.

Sample preparation and CPV 2 screening

Viral DNA was extracted from the clinical samples (either whole blood or rectal swab) using a Genomic DNA Mini Kit (Geneaid Biotech, Ltd., Taipei, Taiwan) according to the manufacturer’s protocol. All of the clinical specimens were screened for CPV 2 by polymerase chain reaction (PCR) as described by Lin et al. [54].

VP2 gene amplification and sequencing

Samples showing positive PCR results from either type of specimen were included in this study. The partial VP2 gene of CPV 2 was amplified by PCR as described by Buonavoglia et al. [9]. The DNA fragments were purified (Geneaid Biotech, Ltd., Taipei, Taiwan), and the target nucleotide sequences were determined in both orientations using an auto-sequencer (ABI 3730XL, Foster City, CA, USA).

Sequence and phylogenetic analyses

The VP2 DNA sequences of our samples were compared to those of reference FPV (M38246), CPV 2 (M38245), CPV 2a (M24003), CPV 2b (M74849, AY742941), new CPV 2a (AY742953, AB054213, AB054215, DQ340434, EF011664, EF189717, EU009204, EU145955, FJ005259, FJ197841, FJ197842, FJ435343, FJ435345, FJ435346, FJ435347, FJ869138, GQ379043, GQ379048, GU569936, GU569942, HQ602978, HQ602995, JF346754, JF906788, JN403045, JX048605, JX048606, KC196114, KF149978), new CPV 2b (AY742955, AY869724, EU009205, EU145954, FJ005260, FJ005264, JF414817, JN867603, KF149985), and CPV 2c (FJ005235, FJ005237, FJ222821, GQ865518, GU380303, JF414818, KC196099, KF149962). Multiple alignments of the nucleic acid and amino acid sequences were performed with the Clustal W method using the MegAlign program (DNASTAR, Madison, WI, USA). The phylogenetic analyses were conducted by the maximum likelihood method using MEGA 5, version 5.05.

Abbreviations

CPV: 

Canine parvovirus

PCR: 

Polymerase chain reaction.

Declarations

Acknowledgement

We thank Dr. Li-En Hsieh, Dr. Ya-Ling Lin, and Dr. Ya-Min Xu for sample collection.

Authors’ Affiliations

(1)
Department of Veterinary Medicine, National Pingtung University of Science and Technology
(2)
Veterinary Hospital, National Pingtung University of Science and Technology
(3)
Graduate Institute of Veterinary Medicine, National Taiwan University
(4)
Don-Da Animal Hospital

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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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