Crystal structure of the lytic CHAPK domain of the endolysin LysK from Staphylococcus aureus bacteriophage K
© Sanz-Gaitero et al.; licensee BioMed Central Ltd. 2014
Received: 28 April 2014
Accepted: 4 July 2014
Published: 26 July 2014
Bacteriophages encode endolysins to lyse their host cell and allow escape of their progeny. Endolysins are also active against Gram-positive bacteria when applied from the outside and are thus attractive anti-bacterial agents. LysK, an endolysin from staphylococcal phage K, contains an N-terminal cysteine-histidine dependent amido-hydrolase/peptidase domain (CHAPK), a central amidase domain and a C-terminal SH3b cell wall-binding domain. CHAPK cleaves bacterial peptidoglycan between the tetra-peptide stem and the penta-glycine bridge.
The CHAPK domain of LysK was crystallized and high-resolution diffraction data was collected both from a native protein crystal and a methylmercury chloride derivatized crystal. The anomalous signal contained in the derivative data allowed the location of heavy atom sites and phase determination. The resulting structures were completed, refined and analyzed. The presence of calcium and zinc ions in the structure was confirmed by X-ray fluorescence emission spectroscopy. Zymogram analysis was performed on the enzyme and selected site-directed mutants.
The structure of CHAPK revealed a papain-like topology with a hydrophobic cleft, where the catalytic triad is located. Ordered buffer molecules present in this groove may mimic the peptidoglycan substrate. When compared to previously solved CHAP domains, CHAPK contains an additional lobe in its N-terminal domain, with a structural calcium ion, coordinated by residues Asp45, Asp47, Tyr49, His51 and Asp56. The presence of a zinc ion in the active site was also apparent, coordinated by the catalytic residue Cys54 and a possible substrate analogue. Site-directed mutagenesis was used to demonstrate that residues involved in calcium binding and of the proposed active site were important for enzyme activity.
The high-resolution structure of the CHAPK domain of LysK was determined, suggesting the location of the active site, the substrate-binding groove and revealing the presence of a structurally important calcium ion. A zinc ion was found more loosely bound. Based on the structure, we propose a possible reaction mechanism. Future studies will be aimed at co-crystallizing CHAPK with substrate analogues and elucidating its role in the complete LysK protein. This, in turn, may lead to the design of site-directed mutants with altered activity or substrate specificity.
KeywordsBacteriophage Calcium Crystallography Endolysin Peptidoglycan Protease Staphylococcus Zinc
Los bacteriófagos codifican endolisinas para lisar sus bacterias hospedadoras y permitir la liberación de su progenie. Las endolisinas también son activas contra bacterias Gram positivas cuando se aplican desde el exterior, y por lo tanto, son consideradas agentes antibacterianos atractivos. LysK, una endolisina del fago K que infecta estafilococos, contiene un dominio N-terminal amidohidrolasa/peptidasa dependiente de cisteína e histidina (CHAPK), un dominio amidasa central y un dominio C-terminal SH3b de unión a la pared bacteriana. CHAPK corta el peptidoglicano bacteriano entre el tetrapéptido y los puentes pentaglicina.
El dominio CHAPK de LysK fue cristalizado y se obtuvieron datos de difracción a alta resolución tanto de un cristal de proteína nativo como de un cristal derivado con cloruro de metilmercurio. La señal anómala presente en los datos derivados permitió la localización de la posición de los átomos pesados y la determinación de la fase. Las estructuras resultantes se completaron, refinaron y analizaron. La presencia de iones de calcio y zinc en la estructura fue confirmada por espectroscopía de emisión de fluorescencia de rayos X. Se llevaron a cabo análisis de zimograma sobre la enzima nativa y sobre mutantes puntuales seleccionados.
La estructura de CHAPK reveló una topología tipo papaína con un bolsillo hidrofóbico donde se localiza la tríada catalítica. Moléculas de tampón ordenadas presentes en este hueco pueden mimetizar el substrato de peptidoglicano. Cuando se compara con dominios CHAP resueltos previamente, CHAPK contiene un lóbulo adicional en su dominio N-terminal, con un ión de calcio estructural, coordinado por los residuos Asp56, Asp45, Asp47. También se observa la presencia de un ión de zinc en el centro activo, coordinado con el residuo catalítico Cys54 y un posible análogo del substrato. Se usó mutagénesis dirigida para demostrar que los residuos involucrados en la unión a calcio y los presentes en el centro activo propuesto eran importantes para la actividad enzimática.
Se determinó la estructura del dominio CHAPK de LysK a alta resolución, sugiriendo la localización del centro activo, del bolsillo de unión al sustrato y revelando la presencia de un ión de calcio estructuralmente importante. Se encontró un ión de zinc unido más débilmente. Basándonos en la estructura, proponemos un posible mecanismo de reacción. Futuros estudios tendrán por objeto la cristalización de CHAPK con análogos del sustrato y la elucidación de su papel en la proteína LysK completa. Esto, a su vez, podría conducir al diseño de mutantes puntuales con una actividad o especificidad de sustrato modificada.
KeywordsBacteriophage Calcium Crystallography Endolysin Peptidoglycan Protease Staphylococcus Zinc
Bacteriophage K is a virulent phage that infects a wide range of staphylococci. It belongs to the Myoviridae family of the Caudovirales order, with a genome of 148,317 bp[1–3]. To allow its progeny to escape from the host cell (“lysis from within”), it encodes the endolysin LysK, a peptidoglycan hydrolase. When applied exogenously to the pathogen, LysK causes “lysis from without” or exolysis. Gram-positive endolysins are highly specific, and no bacterial variants resistant to their phage endolysins have been found despite the use of mutagenesis strategies to promote the chance of resistance development. LysK kills a wide range of staphylococci, including multi-drug-resistant Staphylococcus aureus (MRSA).
LysK contains three domains: an N-terminal cysteine-histidine dependent amido-hydrolase/peptidase (CHAP) domain, a central amidase domain and a C-terminal SH3b cell wall-binding domain. The LysK amidase domain cleaves peptidoglycan between N-acetylmuramic acid and L-alanine of the stem peptide, while the CHAP domain hydrolyzes it between the D-alanine of the tetra-peptide stem and the first glycine of the penta-glycine cross-bridge. A truncated enzyme called CHAPK, containing only the first 165 amino acids of LysK corresponding to the CHAP domain, also showed exolytic activity. CHAPK is able to lyse several staphyloccocal species, independently from their origin, their antibiotic resistance profile and their ability to produce exopolysaccharides (associated with biofilm formation)[10, 11]. It is also effective against other related genera, such as Micrococcus or Streptococcus.
In order to understand the reaction mechanism and perhaps improve or alter the activity, we set out to solve the structure of CHAPK. The CHAPK domain was expressed in Escherichia coli, purified and crystallized. Although the crystallization procedure was not very reproducible and crystals grew as inter-grown plates, a high-resolution dataset could be collected from one of them, plus a dataset from a methylmercury chloride derivative of sufficient quality for structure solution by single-wavelength anomalous dispersion. This structure was refined against both the native and the derivative dataset. Here we present the high-resolution structure of the CHAPK domain solved by X-ray crystallography.
Results and discussion
Refinement and validation statistics for the CHAP K structure
Cell edges (a, b, c, Å)
39.2, 61.5, 73.2
39.0, 61.5, 72.8
Cell angles (α, β, γ, º)
91.5, 98.7, 90.1
91.8, 98.7, 90.0
Resolution range used (Å)
Number of reflections used
Number of reflections used for R-free
Number of atoms (protein/water/other)
Average B-value/Wilson B-value (Å2)
Ramachandran statisticsd (%)
R.m.s. deviationse (bonds, Å/angles, °)
The four CHAPK monomers do not form extensive inter-monomer interfaces in the crystal, suggesting that in solution the protein is monomeric. When the four crystallographically independent monomers are compared with each other, it is observed that they are very similar. While in part this is due to the use of local non-crystallographic symmetry restraints in the refinement, the fact that including these restraints significantly improved correspondence of the model to the data supports the similarity of the four crystallographically independent protein chains. Chains A and B on one hand, and chains C and D on the other, can be most reliably superposed, with root mean square differences (r.m.s.d.) between C-alpha atoms of 0.07 and 0.05 Å, respectively. The r.m.s.d. between chains A or B on one hand and chains C or D on the other are 0.23-0.26 Å. The largest structural differences are concentrated in residues 29–39 and 136–143, part of surface loops that interact with each other. These differences between the monomers are likely caused by interaction with neighbouring monomers in the crystal, i.e. different crystal contacts. The loop consisting of residues 136 to 143 is right next to a putative substrate-binding groove, so it may be somewhat more flexible to allow access of the substrate and release of the cleavage products.
When the structure is analyzed, it is clear that CHAPK belongs to the cysteine protease CA peptidase clan Pfam: CL0125; http://pfam.xfam.org/; Ref., with a papain-like fold. CHAPK is a member of the CHAP family of this clan (Pfam: PF05257), as expected from sequence homology. A structural similarity search revealed that the most similar structure is the CHAP domain of the streptococcal phage endolysin PlyC (PDB entry 4F88), with a root mean square difference (r.m.s.d.) of 2.5 Å when the backbone atoms of 124 residues are superposed onto CHAPK (Z-score 11.4). The next most similar structure is the C-terminal endopeptidase domain of the NlpC/P60 family cell-wall remodelling protein Bacillus cereus PDB code 3H41; Ref., with an r.m.s.d. of 2.8 Å when the backbone atoms of 114 residues are superposed (Z-score 10.2). When the PDB database is searched for sequence-similar structures, the first hit is the CHAP domain from Staphylococcus saprophyticus CHAP domain protein (PDB entry 2K3A), with a sequence identity of 28% over a stretch of 94 residues. However, this structure cannot be superimposed as well as those previously mentioned (r.m.s.d. of 3.4 Å when backbone atoms of 101 residues are superposed, Z-score 6.3) and our attempts to solve the CHAPK structure by molecular replacement using this model were unsuccessful. This lower similarity may be due to the fact that this structure was determined by NMR spectroscopy rather than crystallography. Superposition of the CHAPK structure onto the CHAP domain of the streptococcal phage endolysin PlyC (PDB entry 4F88) is shown in Figure 1C. The two alpha-helices and six beta-strands of CHAPK superpose quite well with the backbone of the homologous structures, but the loops, including the 310-helices, are very different.
Bound metal ions
The calcium ion is bound in the amino-terminal part of the protein, involving residues of the long loop connecting the first and second alpha-helices (residues 17–54) and Asp56 in the second alpha-helix. It is bound in a monodentate way to the side chain of residues Asp45 and Asp47 and in a bidentate way to both oxygen atoms of the Asp56 side chain (Figure 3B). Additional ligands are the main chain oxygen atoms of Tyr49 and His51 and an ordered water molecule. The coordination is octahedral and almost exclusively involves carbonyl oxygen atoms, as expected for calcium. Experimentally determined metal ion-oxygen distances are 2.3-2.5 Å, which is also consistent with usual calcium(II) coordination. The occupancy of the calcium site appears to be complete and the refined temperature factors of the calcium ions are very near those of the coordinating atoms (the temperature factors for the calcium ions vary between 10 and 12 Å2, while those for the coordinating ligand atoms are between 7 and 14 Å2). The calcium ion is near the proposed catalytic site (Figure 2). We propose that the calcium ion plays a structural role, helping to maintain the structure of the amino-terminal domain and thus its catalytic residues in the correct relative orientation. The calcium ion binding loop also contains residues that may be in contact with the substrate and thus play a role in determining substrate specificity. In the derivative protein structure, the calcium is present at the same occupancy and with the same coordinating ligands.
In contrast to the tightly bound calcium ion, the zinc ions appear to be bound more loosely and the derivative structure shows they could be replaced by methylmercury ions upon soaking of the crystals with methylmercury chloride. Also, the occupancy appears to be less than unity, we estimate it to be around 0.67 based on refinement runs performed at different occupancies. Finally, the resulting electron density around the zinc ions is somewhat ambiguous and we could not model the ligands without some remaining uncertainty. The zinc ions are coordinated by the sulphydryl group of Cys54, the sulphate group of the bound MES and several water molecules (Figure 3C). It is also near the main chain oxygen atom of Gly116. The coordination distances for the zinc ion are not ideal; the zinc ion is too close to Cys54 and too far from the coordinating oxygen atoms. A report by another group showed that zinc ions inhibit the LysK enzyme, while calcium ions have no effect on activity, but significantly enhance stability of the enzyme. However, in this assay, metal ions were not removed from the protein solution prior to testing their effects on the enzyme. Zinc ions may play a regulatory role, and their binding near Cys54 suggests they may regulate access of the substrate to the catalytic site.
Catalytic centre and proposed reaction mechanism
Mutation of the conserved Cys54 and His117 residues to alanine resulted in complete elimination of staphylolytic activity of the enzyme as demonstrated by zymographic analysis, indicating an essential role of these residues and supporting the hypothesis that they are part of the catalytic triad. Glu134 is believed to be the other residue of the catalytic triad, but is not as highly conserved as the other two residues. When this residue was mutated to alanine, it was clear from zymogram results that, although the catalytic activity was not completely eliminated, it was strongly reduced. In the absence of Glu134 perhaps another residue can take over its role.
A likely mechanism of action, analogous to that of other papain proteases[23, 24], is the following: Glu134 accepts a proton from the protonated imidazole group of His117. His117 subsequently accepts a proton from the hydroxyl group of Cys54 (through its N-epsilon). The deprotonated Cys54 then performs a nucleophilic attack on the peptidic bond between D-Ala and Gly in the staphylococcal peptidoglycan. As a result, a transacylation reaction between the enzyme and substrate occurs, giving rise to an acyl-enzyme intermediate. This intermediate may be hydrolyzed to release the enzyme and the cleaved peptidoglycan. In the NlpC/P60 domain of lipoprotein SPR from E. coli, there is a tyrosine residue (Tyr56) that has been reported to be very conserved and which may modulate Cys nucleophilicity or help in substrate binding. In the case of CHAPK, Tyr140 is located in an equivalent position, but having a different role, since its phenol group is pointing in the opposite direction. Cysteine proteases have an oxyanion hole, which helps to stabilize the developing negative charge during the formation of the acylenzyme intermediate. Asn136, which is located in close proximity to the catalytic triad, is one residue hypothesized to be involved in creating the oxyanion hole. When this residue was mutated to an alanine, the activity of the enzyme was visibly reduced, but not completely eliminated, supporting the aforementioned hypothesis.
Comparison with LysGH15 CHAP domain structure
While this manuscript was under review, a paper describing the structures of the CHAP domain (PDB entry 4 OLK), amidase-2 domain (PDB entry 4OLS) and the SH3 domain (PDB entry 2MK5) of the endolysin LysGH15 from phage GH15 was published. The first two were solved by X-ray crystallography at 2.7 and 2.2 Å resolution respectively, while the latter was solved by NMR spectroscopy. Phages GH15 and K share 97% identity in 84% of their genomes (Genbank entries NC_019448 and NC_005880, respectively)[2, 28]. The LysGH15 and LysK protein sequences are virtually identical, with only four amino acid differences in their 495-residue sequences. Of the differences, two are in the CHAP domain: Val26 of CHAPK is an isoleucine in CHAPGH15 and Glu113 of CHAPK is a glutamine in CHAPGH15. The high sequence similarity means the enzymes are almost identical and expected to share the same properties.
When the crystal structures of the CHAP domains are compared, it is notable the spacegroups and crystal packing are very different, which suggests the protein is a monomer in solution and inter-monomer interactions in the crystal are not likely to be biologically relevant. Given the almost identical sequences, it is not surprising that the monomer structures are highly similar; superposition of the two CHAP domains leads to an r.m.s.d. of 0.3 Å when 139 C-alpha atoms are superposed. The only significant difference in main-chain conformation is present in residues 109–116, which follow a different path in the two structures. This may indicate that this loop, which is directed away from the active site, is flexible and of limited importance to the structure and activity of the enzyme. The large side-chains of Tyr49, Trp73, Tyr140 and Tyr153, which are all on the surface of the protein, show different orientations.
The higher resolution of the CHAPK structure when compared to the CHAPGH15 structure (1.8 vs. 2.7 Å) should have led to more accurate placement of side-chain atoms and solvent molecules. In both structures, a buffer molecule occupies the groove that likely accommodates the peptidoglycan substrate: a Bis-Tris molecule (2-[Bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)-1,3-propanediol) in between the two monomers of the asymmetric unit of CHAPGH15 and a MES and HEPES molecule in the case of the native and derivative structures of CHAPK, respectively. The calcium ion is in exactly the same position, as are its coordinating residues and the EF-hand-like domain in which it is incorporated. No zinc ion was observed in the CHAPGH15 crystals.
Gu et al. also performed site-directed mutagenesis studies, but on the intact LysGH15 enzyme, not on the isolated CHAPGH15 domain. As observed for CHAPK, it was found that mutating the active site residue Cys54 affected bacterial lysis activity strongly. Mutating the calcium ion coordinating residues Asp45, Asp46 and Asp56 also diminished activity about ten-fold, while Tyr49 and His51 seem less important, the same as we observed.
We determined the structure of the CHAPK domain of LysK at 1.8 Å resolution (1 Å = 0.1 nm). The structure has the papain-type fold with a long loop between the two amino-terminal alpha-helices. The structure suggests the location of the active site near a hydrophobic groove, with Cys54, His117 and Glu134 forming the catalytic triad. The substrate most likely binds to the hydrophobic groove.
A calcium ion was found tightly bound to the protein. Its ligands are the side-chains of Asp45, Asp47 and Asp56, plus the backbone oxygens of Tyr49 and His51, all in the amino-terminal domain specific to CHAPK. It likely has a structural role, stabilizing the protein fold. It may also be involved in ensuring the correct location of the peptidoglycan inside the catalytic cleft or in the stabilization of the negative charge of the tetrahedral intermediate during catalysis. A zinc ion was also found and is likely more loosely bound, as it is less buried, has less protein ligands and could be exchanged for a methylmercury ion upon derivatization. Its role, if any, may be regulatory.
Based on the structure, we propose a possible reaction mechanism, involving all three residues of the likely catalytic triad. Future studies will include co-crystallization with peptidoglycan analogues and elucidating the role of the CHAPK domain in the complete LysK protein. This may allow site-directed mutation to modulate the peptidoglycan specificity and activity of both the CHAPK and LysK enzymes.
CHAPK was expressed, purified, crystallized and crystallographic data was collected as described[9, 12]. A complete native dataset was collected to 1.8 Å resolution with good statistics. A dataset to 1.7 Å resolution, but with inferior completeness, was also collected from a methylmercury chloride derivative at the Hg L-I edge. However, this dataset allowed phase determination by single anomalous dispersion (SAD) and automatic model building of four crystallographically independent protein molecules in the P1 unit cell (Table 1) using the ARP-WARP program. The model was refined against the derivative dataset and separately against the native dataset. The models were completed and adjusted using COOT and refined with REFMAC5, using local non-crystallographic symmetry restraints and taking care to select the same reflections for calculation of Rfree. To confirm the presence of zinc and calcium ions in the sample, an X-ray fluorescence emission spectrum was collected on a native protein crystal at ESRF beamline ID23-1. Validation was performed with MolProbity. Refinement and validation statistics are shown in Table 1.
Crystal contact analysis was done with PISA; other analyses were performed with the CCP4 suite. Structural similarity analysis was performed with DALI; for plotting a protein surface coloured according to amino acid conservation, CONSURF was used. The pKa of selected residues in the protein structure was predicted with PROPKA. The structural models and underlying data files have been submitted to the PDB (accession code 4CSH for the native structure and 4CT3 for the derivative). PYMOL (Schrödinger LLC, Portland OR, USA) was used for making structure figures and TOPDRAW to draw the secondary structure diagram.
CHAPK mutants were created using the QuikChange II Site-Directed Mutagenesis Kit from Agilent (Santa Clara CA, USA) as per the manufacturer’s instructions. Crude cell lysate was analyzed for over-expression using sodium dodecyl sulphate gel electrophoresis and for ability to lyse Staphylococcus aureus cells using zymographic gels as described previously.
We thank Jordi Benach (ALBA beamline BL13/XALOC), Max Nanao (ESRF beamline ID23-2), Christian Perrin (ESRF), Christoph Mueller-Dieckmann (ESRF beamline ID23-1) and James Sandy (DLS beamline I02) for help with using synchrotron data collection facilities. We acknowledge ALBA/CELLS (proposal number 2012010140), the European Synchrotron Radiation Facility (proposal number MX1477) and the Diamond Light Source (proposal number MX3808), which contributed to the results presented here. The research leading to these results has received funding from the European Community’s Seventh Framework Programme (FP7/2007-2013) under BioStruct-X grant agreement no. 283570 and was sponsored by grant BFU2011-24843 (MJvR) from the Spanish Ministry of Economy and Competitiveness, a Masters fellowship (MSG) and an FPU Ph.D. fellowship (CGD) from the Spanish Ministry of Education, Culture and Sports. We also acknowledge financial support from TSR-StrandIII:CRS/07/CR03 and FIRM:08RDCIT600 of the Irish Department of Agriculture.
- Rees PJ, Fry BA: The morphology of staphylococcal bacteriophage K and DNA metabolism in infected Staphylococcus aureus . J Gen Virol 1981,53(Pt 2):293-307.PubMedView Article
- O’Flaherty S, Coffey A, Edwards R, Meaney W, Fitzgerald GF, Ross RP: Genome of staphylococcal phage K: a new lineage of Myoviridae infecting Gram-positive bacteria with a low G + C content. J Bacteriol 2004,186(9):2862-2871.PubMedPubMed CentralView Article
- Gill JJ: Revised genome sequence of staphylococcus aureus bacteriophage K. Genome Announcements 2014,2(1):e01173.PubMedPubMed CentralView Article
- Loessner MJ: Bacteriophage endolysins - current state of research and applications. Curr Opin Microbiol 2005,8(4):480-487.PubMedView Article
- Ralston DJ, McIvor M: Lysis-from-without of Staphylococcus aureus strains by combinations of specific phages and phage-induced lytic enzymes. J Bacteriol 1964, 88: 676-681.PubMedPubMed Central
- Loeffler JM, Nelson D, Fischetti VA: Rapid killing of Streptococcus pneumoniae with a bacteriophage cell wall hydrolase. Science 2001,294(5549):2170-2172.PubMedView Article
- O’Flaherty S, Coffey A, Meaney W, Fitzgerald GF, Ross RP: The recombinant phage lysin LysK has a broad spectrum of lytic activity against clinically relevant staphylococci, including methicillin-resistant Staphylococcus aureus . J Bacteriol 2005,187(20):7161-7164.PubMedPubMed CentralView Article
- Becker SC, Dong S, Baker JR, Foster-Frey J, Protchard DF, Donovan DM: LysK CHAP endopeptidase domain is required for lysis of live staphylococcal cells. FEMS Microbiol Lett 2009,294(1):52-60.PubMedView Article
- Horgan M, O’Flynn G, Garry J, Cooney J, Coffey A, Fitzgerald GF, Ross RP, McAuliffe O: Phage lysin LysK can be truncated to its CHAP domain and retain lytic activity against live antibiotic-resistant staphylococci. Appl Environ Microbiol 2009,75(3):872-874.PubMedPubMed CentralView Article
- Fenton M, Casey PG, Hill C, Gahan CG, Ross RP, McAuliffe O, O’Mahony J, Maher F, Coffey A: The truncated phage lysin CHAP(k) eliminates Staphylococcus aureus in the nares of mice. Bioeng Bugs 2010,1(6):404-407.PubMedPubMed CentralView Article
- Fenton M, Keary R, McAuliffe O, Ross RP, O’Mahony J, Coffey A: Bacteriophage-derived peptidase CHAP K eliminates and prevents staphylococcal biofilms. Internat J Microbiol 2013, 2013: 625341.View Article
- Sanz-Gaitero M, Keary R, Garcia-Doval C, Coffey A, van Raaij MJ: Crystallization of the CHAP domain of the endolysin from Staphylococcus aureus bacteriophage K. Acta Crystallogr Sect F Struct Biol Cryst Commun 2013,69(Pt 12):393-1396.
- Fenton M, Cooney JC, Ross RP, Sleator RD, McAuliffe O, O’Mahony J, Coffey A: In silico modeling of the staphylococcal bacteriophage-derived peptidase CHAP K . Bacteriophage 2011,1(4):198-206.PubMedPubMed CentralView Article
- Punta M, Coggill PC, Eberhardt RY, Mistry J, Tate J, Boursnell C, Pang N, Forslund K, Ceric G, Clements J, Heger A, Holm L, Sonnhammer EL, Eddy SR, Bateman A, Finn RD: The Pfam protein families database. Nucl Acids Res 2012, 40: D290-D301.PubMedPubMed CentralView Article
- McGowan S, Buckle AM, Mitchell MS, Hoopes JT, Gallagher DT, Heselpoth RD, Shen Y, Reboul CF, Law RHP, Fischetti VA, Whisstock JC, Nelson DC: X-ray crystal structure of the streptococcal specific phage lysin PlyC. Proc Natl Acad Sci U S A 2012,109(31):12752-12757.PubMedPubMed CentralView Article
- Xu Q, Abdubek P, Astakhova T, Axelrod HL, Bakolitsa C, Cai X, Carlton D, Chen C, Chiu HJ, Chiu M, Clayton T, Das D, Deller MC, Duan L, Ellrott K, Farr CL, Feuerhelm J, Grant JC, Grzechnik A, Han GW, Jaroszewski L, Jin KK, Klock HE, Knuth MW, Kozbial P, Krishna SS, Kumar A, Lam WW, Marciano D, Miller MD, et al.: Structure of the c-D-glutamyl-L-diamino acid endopeptidase YkfC from Bacillus cereus in complex with L-Ala-c-D-Glu: insights into substrate recognition by NlpC/P60 cysteine peptidases. Acta Crystallogr Sect F Struct Biol Cryst Commun 2010,66(Pt 10):1354-1364.PubMedPubMed CentralView Article
- Rossi P, Aramini JMR, Xiao R, Chen CX, Nwosu C, Owens LA, Maglaqui M, Nair R, Fischer M, Acton TB, Honig B, Rost B, Montelione GT: Structural elucidation of the Cys-His-Glu-Asn proteolytic relay in the secreted CHAP domain enzyme from the human pathogen staphylococcus saprophyticus . Proteins 2009,74(2):515-519.PubMedPubMed CentralView Article
- Jones KW, Gordon BM, Hanson AL, Kwiatek WL, Pounds JG: X-ray fluorescence with synchrotron radiation. Ultramicroscopy 1988,24(2–3):313-328.PubMedView Article
- Harding MM: Small revisions to predicted distances around metal sites in proteins. Acta Crystallogr Sect D Biol Crystallogr 2006,62(Pt 6):678-682.View Article
- Filatova LY, Becker SC, Donovan DM, Gladilin AK, Klyachko NL: LysK, the enzyme lysing staphylococcus aureus cells: specific kinetic features and approaches towards stabilization. Biochimie 2010, 92: 507-513.PubMedView Article
- Fenton M, Ross RP, McAuliffe O, O’Mahony J, Coffey A: Characterization of the staphylococcal bacteriophage lysin CHAP K . J Appl Microbiol 2011, 111: 1025-1035.PubMedView Article
- Aramini JM, Rossi P, Huang YJ, Zhao L, Jiang M, Maglaqui M, Xiao R, Locke J, Nair R, Rost B, Acton TB, Inouye M, Montelione GT: Solution NMR structure of the NlpC/P60 domain of lipoprotein Spr from Escherichia coli: structural evidence for a novel cysteine peptidase catalytic triad. Biochemistry 2008,47(37):9715-9717.PubMedView Article
- Shokhen M, Khazanov N, Albeck A: The mechanism of papain inhibition by peptidyl aldehydes. Proteins 2011, 79: 975-985.PubMedView Article
- Brömme D: Papain-like cysteine proteases. Curr Protoc Protein Sci 2001, 21: 21.2.1-21.2.14.
- Lau EY, Bruice TC: Consequences of breaking the Asp-His hydrogen bond of the catalytic triad: effects on the structure and dynamics of the serine esterase cutinase. Biophys J 1999,77(1):85-98.PubMedPubMed CentralView Article
- Menard R, Storer AC: Oxyanion hole interactions in serine and cysteine proteases. Biol Chem Hoppe Seyler 1992,373(7):393-400.PubMedView Article
- Gu J, Feng Y, Feng X, Sun C, Lei L, Ding W, Niu F, Jiao L, Yang M, Li Y, Liu X, Song J, Cui Z, Han D, Du C, Yang Y, Ouyang S, Liu ZJ, Han W: Structural and biochemical characterization reveals LysGH15 as an unprecedented “EF-hand-like” calcium-binding phage lysin. PLOS Path 2014,10(5):e1004109.View Article
- Gu J, Liu X, Lu R, Li Y, Song J, Lei L, Sun C, Feng X, Du C, Yu H, Yang Y, Han W: Complete genome sequence of staphylococcus aureus bacteriophage GH15. J Virol 2012,86(16):8914-8915.PubMedPubMed CentralView Article
- Langer G, Cohen SX, Lamzin VS, Perrakis A: Automated macromolecular model building for X-ray crystallography using ARP/wARP version 7. Nat Protoc 2008,3(7):1171-1179.PubMedPubMed CentralView Article
- Emsley P, Cowtan K: Coot: model-building tools for molecular graphics. Acta Crystallogr Sect D Biol Crystallogr 2004,60(Pt 12 Pt 1):2126-2132.View Article
- Murshudov GN, Skubak P, Lebedev AA, Pannu NS, Steiner RA, Nicholls RA, Winn MD, Long F, Vagin AA: Refmac5 for the refinement of macromolecular crystal structures. Acta Crystallogr Sect D Biol Crystallogr 2011,67(Pt 4):355-367.View Article
- Brünger A: Free R value: a novel statistical quantity for assessing the accuracy of crystal structures. Nature 1992,355(6359):472-475.PubMedView Article
- Leonard GA, Solé VA, Beteva A, Gabadinho J, Guijarro M, McCarthy J, Marrocchelli D, Nurizzo D, McSweeney S, Mueller-Dieckmann S: Online collection and analysis of X-ray fluorescence spectra on the macromolecular crystallography beamlines of the ESRF. J Appl Crystallogr 2009, 42: 333-335.View Article
- Chen VB, Arendall WB 3rd, Headd JJ, Keedy DA, Immormino RM, Kapral GJ, Murray LW, Richardson JS, Richardson DC: MolProbity: all-atom structure validation for macromolecular crystallography. Acta Crystallogr Sect D Biol Crystallogr 2010,66(Pt 1):12-21.View Article
- Krissinel E, Henrick K: Inference of macromolecular assemblies from crystalline state. J Mol Biol 2007,372(3):774-797.PubMedView Article
- Winn MD, Ballard CC, Cowtan KD, Dodson EJ, Emsley P, Evans PR, Keegan RM, Krissinel EB, Leslie AG, McCoy A, McNicholas SJ, Murshudov GN, Pannu NS, Potterton EA, Powell HR, Read RJ, Vagin A, Wilson KS: Overview of the CCP4 suite and current developments. Acta Crystallogr Sect D Biol Crystallogr 2011,67(Pt 4):235-242.View Article
- Holm L, Rosenström P: Dali server: conservation mapping in 3D. Nucleic Acids Res 2010,38(Web Server issue):W545-W549.PubMedPubMed CentralView Article
- Armon A, Graur AD, Ben-Tal N: ConSurf: an algorithmic tool for the identification of functional regions in proteins by surface mapping of phylogenetic information. Bioinformatics 2003,19(1):163-164.View Article
- Rostkowski M, Olsson MHM, Søndergaard CR, Jensen JH: Graphical analysis of pH-dependent properties of proteins predicted using PROPKA. BMC Struct Biol 2011, 11: 6.PubMedPubMed CentralView Article
- Bond CS: Topdraw: a sketchpad for protein structure topology cartoons. Bioinformatics 2003,19(2):311-312.PubMedView Article
- Keary R, McAuliffe O, Ross RP, Hill C, O’Mahony J, Coffey A: Genome analysis of the staphylococcal temperate phage DW2 and functional studies on the endolysin and tail hydrolase. Bacteriophage 2014,4(1):e28451.PubMedPubMed CentralView Article
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