The placental specific gene, PLAC1, is induced by the Epstein-Barr virus and is expressed in human tumor cells
© Wang et al.; licensee BioMed Central Ltd. 2014
Received: 5 February 2014
Accepted: 30 April 2014
Published: 9 June 2014
The Epstein-Barr virus (EBV) is a causal agent in a number of malignancies in humans including hematopoietic tumors and non-hematopoietic tumors. Burkitt’s lymphoma cell lines containing the Epstein-Barr virus have been shown to form tumors in nude mice while clonal derivatives of such cell lines in which the viral genome has been lost do not (JID 177: 1194-1201, 1998; JV 72: 9150-9156, 1998; JV 68: 6069-6073, 1994). The re-introduction of EBV into these EBV negative BLs reconstitutes the tumor phenotype. Thus, EBV-induced cellular genes play critical role in EBV-related tumors.
Methods and results
In an attempt to identify cellular genes regulated by EBV that may contribute to its tumorigenic properties, we have enforced genome loss in the Burkitt’s lymphoma (BL) line, MutuI, by introducing a dominant negative form of the episomal replication factor, EBNA1 and carried out gene array analysis. One of the genes identified by this analysis is PLAC1, a gene originally identified as being expressed exclusively in placental tissue. Real time RT-PCR analysis verified higher expression in EBV positive vs. EBV negative Mutu clones. Analysis of a panel of RNAs from 20 normal tissues demonstrated the highest level of expression in placenta but significant expression was also observed in testis and brain cerebellum. PLAC1 expression was also observed in non-BL tumor cell lines derived from breast, ovary, and prostate. Lastly, expression of PLAC1 was found to be higher in some primary breast tumors compared to normal adjacent tissues.
This data suggests that the EBV-induced PLAC1 is a member of the cancer/testis group of tumor antigens.
KeywordsEBV PLAC1 Cancer Breast Ovarian Prostate
Placental specific gene 1 (Plac1) is a member of a family of genes that have been identified as showing predominantly placenta specific gene expression [1–5] with expression being restricted to cells of trophoblastic lineage in the mouse and human [1, 2]. Trophoblasts play numerous key roles in fetal development determined, in part, by differentiating along distinct pathways. These include the controlled invasion of extravillus trophoblasts into maternal uterine tissue resulting in placental attachment and regulatory interactions with the uterine vasculature. Additionally, multinucleated syncytiotrophoblasts are metabolically active cells that are in direct contact with the maternal circulation. They are responsible for many of the functions essential to pregnancy maintenance, i.e. nutrient and oxygen transport to the fetus, hormone production and secretion, and elimination of fetal waste products. As a support mechanism for these latter functions, trophoblasts specifically promote angiogenesis in part through the production of human chorionic gonadotropin (hCG) and vascular endothelial growth factor (VEGF) [6, 7]. Lastly, the placenta also contributes to immune tolerance necessary for effective placentation and pregnancy maintenance.
The PLAC1 gene is located on the X chromosome (Xq26.3) near the hypoxanthine-guanine phosphoribosyl transferase (HPRT) gene . PLAC1 is a 212 amino acid protein containing a predicted amino terminal transmembrane domain and a cleavable signal peptide of 23 amino acids (with a cleavage site following amino acid 23 ). PLAC1 has homology to the zona pellucida 3 protein (ZP3), a sperm binding glycoprotein which confers species specificity during fertilization , and has a “zona pellucida sperm-binding protein signature” between amino acids 89 and 106. Analysis of RNAs from a panel of normal tissues showed predominantly placental expression. While little is known regarding the function of PLAC1, lower circulating levels of PLAC1 mRNA in maternal serum have been shown to be associated with vaginal bleeding and threatened abortion prior to week 20 suggesting a key role for PLAC1 in trophoblast function . Recently, PLAC1 is recognized as an essential factor for normal placental and embryonic development in mutant mouse model .
The identification of tumor specific antigens for the development of possible immunological cancer vaccines has been of significant interest. The accumulated identification of tumor specific antigens led to the recognition of a wide array of genes of diverse function but sharing a number of unique characteristics  including expression in normal tissue being restricted to testis (and often placenta), expression in a number of cancers, association with tumor grade, and immunogenicity in cancer patients . In addition, many of these genes have been found to be localized on the X chromosome. Based on these common characteristics of genes with otherwise diverse functions led to their being classified together and referred to as “cancer/testis antigens (CT antigens)” . Due to its localization on the surface of cancer cells, PLAC1 provides accessibility to antibodies make them attractive candidates for targeted immunotherapeutic approaches for breast cancer and other tumor types .
The Epstein-Barr virus (EBV) is a causal agent in a number of malignancies in humans including nasopharyngeal carcinoma, the endemic form of Burkitt’s lymphoma (BL), Hodgkin’s disease, and a small percentage of gastric carcinomas [12, 13]. EBV is especially problematic in immuno-compromised individuals and despite the utilization of highly active retroviral therapy (HAART) to treat AIDs patients, the incidence of EBV associated non-Hodgkin’s lymphomas have increased [14, 15].
In most tumors, EBV exists as an autonomously replicating episome. Episomal replication and segregation during mitosis is facilitated in part by the EBV encoded nuclear antigen, EBNA1 (E pstein-B arr virus n uclear a ntigen 1) [16, 17]. Previous studies have shown that either long term culture, clonal selection, or treatment with hydroxyurea can lead to the loss of EBV episomes in EBV positive Burkitt’s lymphoma cell lines [18–20]. While these EBV negative derivatives proliferate in tissue culture, the parental BLs form colonies in soft agar and form tumors in nude mice, but the EBV negative derivatives do not. Further, the re-introduction of EBV into these EBV negative BLs reconstitutes the tumor phenotype. In an effort to identify cellular genes regulated by EBV that may contribute to the malignant phenotype, we enforced EBV genome loss in the EBV positive BL line, MutuI, and carried out gene array analysis of the resulting EBV negative clones vs. the EBV positive progenitor cells. Here we report that one of the genes identified in this analysis is PLAC1. We also show that this gene is expressed in testis and that it is expressed in other tumors not associated with EBV. Based on its expression profile and its localization to the X chromosome, we propose that PLAC1 is a candidate CT antigen.
We show here that like previous studies [1, 2], PLAC1 expression is highly limited in normal tissues. Nevertheless, using real time RT-PCR, we have observed that PLAC1 expression can be detected in testis and cerebellum. PLAC1’s higher level of expression in EBV positive MutuI cells relative to EBV negative Mutu cells led us to test the expression PLAC1 in a panel of tumor cell lines. This analysis revealed that PLAC1 is highly expressed in several Burkitt’s lymphoma cell lines as well as tumor cell lines derived from a number of other cancers. Using RNAs isolated from primary matched tumor and normal tissues, we further determined a higher level of PLAC1 expression is some tumor samples. This raises the possibility that PLAC1 may be a member of the expanding group of CT antigens.
The first CT antigens described were identified using screens to identify antigenic proteins in cancer patients that are specifically expressed in tumor cells with the hopes of identifying targets for immunotherapy. Another common but not exclusive feature of CT antigens is their inducibility by hypomethylating agents and/or histone deacetylase inhibitors. At this time we have not tested this issue but it is notable that the promoter for PLAC1 is devoid of CpG dinucleotides and therefore may not be subjected to CpG methylation dependent silencing. Nevertheless, it does seem likely that PLAC1 might be silenced through some epigenetic imprinting mechanism involving histone deacetylases.
There are several functions that both the placenta and tumors provide which are required for and/or enhance the survival of the fetus and the tumor. The placenta is the key organ responsible for the exchange of nutrients and waste materials to and from the fetus. While these activities are mediated by specific transport mechanisms, this process is enhanced through the synthesis of angiogenesis promoting factors such as vascular endothelial growth factor (VEGF) and human chorionic gonadotropin (hCG) by the trophoblast [6, 7]. Similarly, hypoxic conditions in tumors can promote the synthesis of angiogenic factors such as VEGF, which in turn leads to angiogenesis. Another key function of the placenta is to protect the fetus from immuno-surveillance by the mother. Both the fetus and the placenta are of fetal origin and therefore are immunologically distinct from the mother. The placenta utilizes multiple mechanisms to prevent immunological targeting by the mother’s immune system including the down regulation of major histocompatibility complex (MHC) II proteins . Similarly, tumors have been shown to down regulate MHC proteins . Tumors can also secrete cytokines that enhance the recruitment of tolerance inducing immune cells  and this also appears to be a key mechanism utilized by the placenta to prevent fetal targeting . Another commonality between the synciotrophoblasts and invasive tumors is their production of metalloproteinases (MMPs) which serve to promote the invasive properties of the tumor and of the synciotrophoblasts .
Other studies have previously found molecular links between trophoblasts and malignant cells. Acevedo et al. discovered that 28 out of 28 randomly selected cancer cell lines express a membrane form of hCG . In another study, Goshen et al. found that a specific splice variant of CD44 which is found in metastasizing human malignancies and is thought to play an important role in metastatic spread, is also expressed in invasive extravillous trophoblasts . These authors propose that this splice variant may similarly play a role in trophoblast invasion. Interestingly, a previous study also found that another Epstein-Barr virus induced gene, EBI3 (Epstein-Barr virus inducible 3) is expressed at high levels in the syncytiotrophoblasts [31, 32]. Together, there is accumulating data indicating that there are not only numerous functional similarities between tumors and the placenta but that there are also some similarities in the mechanisms, which mediate these common functions. The findings that PLAC1 is expressed in both tumors and in trophoblasts provide an additional molecular link between the two. While there is a limited knowledge regarding the function of PLAC1, there is circumstantial evidence indicating that it is crucial for fetal development  and for embryonic development . Knockdown of PLAC1 in breast cancer cells not only blocks cell cycle and proliferation, but also impairs cell motility, migration and invasion probably through inhibiting the cyclin D1 and the phosphorylation of AKT kinase . It will be interesting to ultimately determine how PLAC1 expression functions and whether it similarly promotes one or more key survival functions in tumors.
The number of latency associated genes expressed in EBV associated tissues varies according to the tumor and cell type. Typically, primary Burkitt’s lymphomas express a limited set of viral genes: EBNA1, two small non-coding Pol III derived transcripts referred to as EBER1 and EBER2, and a set of alternatively spliced products derived from the BamHI A region of the EBV genome (latency type I genes) . In EBV immortalized non-tumor lymphoblastoid cell lines, a larger group of EBV encoded genes are expressed (latency type III genes) which are important for maintaining the growth phenotype of the infected cell . In this case, the EBV encoded genes, EBNA1, EBERs, BamHI transcripts, plus EBNA2, EBNA-LP, EBNA3A, EBNA3B, EBNA3C and the latent membrane proteins, LMP1 and LMP2 are expressed. Many of the EBV genes unique to the type III program (relative to the type I program) are each essential for providing the growth phenotype to lymphoblastoid cell lines. In line with this, the growth phenotype of Burkitt’s lymphoma cells is probably dictated principally through genetic alterations such as the c-Myc translocation to the immunoglobulin locus , the defining translocation of Burkitt’s lymphomas. Accordingly, the EBV genome can be eradicated in Burkitt’s lymphoma cell lines without abrogating the proliferative capacity of the derived cells [18–20]. Nevertheless, it is clear that type I gene expression contributes to the tumor phenotype of Burkitt’s lymphoma cells since the loss of EBV in these cells results in cells that cannot form colonies in soft agar and cannot form tumors in SCID mice. This indicates that the type I genes likely play key roles in tumorigenesis other than simply promoting cell growth and it is possible that type I EBV gene expression contributes some of the characteristics that are common to tumors and placenta outlined above. It is notable that of the B-cell lines tested, the highest expression of PLAC1 was observed in the type I BL lines (note: although P3HR1 is a Burkitt’s lymphoma derived cell line, these cells display a group II/III gene expression pattern). This indicates that induction of PLAC1 by EBV is likely facilitated principally by the type I gene expression pattern. It will be interesting to determine whether expression of PLAC1 might contribute to one or more of the tumorigenic properties of EBV.
Enforced genome loss of EBV and carried out gene array and real time RT-PCR analysis, we identify that EBV induces PLAC1 gene. Based on its expression profile and its localization to the X chromosome, we propose that PLAC1 is a candidate CT antigen.
Materials and methods
All B-cell lines, LNCAP and PC3 cell lines were propagated in RPMI supplemented with 10% fetal bovine serum albumin, penicillin, streptomycin, and glutamine. Hela, 293, MDA-MB-231, MDA-MD-361, CaoV3, and BG1 cells were propagated in Dulbecco’s Modified Eagles Medium (DMEM; Life Technologies) supplemented with 10% fetal bovine serum albumin, penicillin, streptomycin, and glutamine. Cells were cultured at 37°C in a humidified 5% CO2-containing atmosphere.
Transient transfection experiments were performed by using a modified version of the calcium phosphate precipitation procedure (a detailed protocol is available at flemingtonlab.com). Briefly, 106 cells were plated onto 100-mm-diameter tissue culture dishes. The following day, the medium was replaced with 8 ml of fresh supplemented DMEM; 4 h later, DNA precipitates were generated by mixing 0.5 ml of 1x HEPES-buffered saline (0.5% HEPES, 0.8% NaCl, 0.1% dextrose, 0.01% anhydrous Na2HPO4, 0.37% KCl [pH 7.10]) with a total of 30 μg of plasmid DNA (10 μg retroviral vector plus 10 μg VSV-G expression vector, plus 10 μg pVPACK dGI packaging vector). A total of 30 μl of 2.5 M CaCl2 was added, and samples were mixed immediately. Precipitates were allowed to form at room temperature for 20 min before being added drop wise to cells. Cells were incubated at 37°C with 5% CO2 for 16 h before the medium was replaced with 10 ml of fresh DMEM (plus 10% FBS).
24 hrs later, viral supernatants were collected and subjected to two rounds of centrifugation to get rid of floating 293 cells. Infections were carried out in 6 well plates with 1 ml virus plus 106 MutuI or DG75 cells suspended in 1 ml DME (+10% fetal bovine serum). Polybrene was added to a final concentration of 8 ug/ml and the mixture was mixed by gently rocking. Cells were spun in 6 well plates at 1000 g for 1 hr followed by 4 hr incubation in a tissue culture incubator. Cells were then collected, spun down and resuspended in 2 ml RPMI (+10% fetal bovine serum, + penicillin, streptomycin, and glutamine) per well. Cells were cultured for 2 days prior to antibiotic selection.
Western blot analysis
After a single 1× PBS wash, cells were immediately suspended in 300 μl of sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis loading buffer (125 mM Tris [pH 6.80], 10% glycerol, 2% SDS, 5% 2-mercaptoethanol, 0.05% bromphenol blue) and boiled for 20 min to shear the genomic DNA. Whole-cell extracts were measured with the Bio-Rad protein assay kit according to the manufacturer's instructions. An equal weight of cell lysates was subjected to SDS-polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes. The blots were blocked for 30 min in Tris-buffered saline containing 5% low-fat powdered milk and 1% FBS and then incubated with the primary antibody (in blocking buffer) overnight at 4°C. The blots were washed three times with 1× TBST (140 mM NaCl, 3 mM KCl, 25 mM Tris–HCl [pH 7.4], 0.1% Tween 20) (each wash was carried out for approximately 10 min). The blots were then incubated with horseradish peroxidase-conjugated secondary antibody (Bio-Rad) in blocking buffer for 1 h at room temperature. Blots were washed as described above and analyzed with an enhanced chemiluminescence detection system (Perkin-Elmer) according to the manufacturer's recommendations, and filters were exposed to Fuji Super RX film. The following antibodies were used for Western blot analysis: EBNA1 (Advanced Biotechonologies Incorporated, CAT# 13-156-100, 1:1000 dilution), HA.11 (Boehringer Mannheim, Cat# 1 867 431, 1:1000 dilution), Actin (Sigma, Cat# A 4700, 1:1000 dilution).
Real time RT-PCR
RNAs from cells were prepared using a RNeasy® Mini kit from Qiagen (Cat# 74104) according to vendor’s protocol. Normal tissue RNAs were purchased from BD Biosciences (Cat# 636643). Individual RNAs from primary lung (Cat#64013-1), colon (Cat#64014-1), and breast (Cat#64015-1) tumors were purchases from BD Biosciences. qPCR human matched pair total RNA panel was purchased from BD Biosciences (Cat# 636691).
cDNA synthesis was carried out using Superscript™ III first-strand synthesis system for RT-PCR (Invitrogen, Cat#. 18080–051). 2 ug of RNA were used for first strand synthesis and then diluted to a final volume of 200 ul.
Real Time PCR was carried out using 5 ul of diluted cDNA for each 20 ul reaction. Analysis was carried out on a BioRad iCycler using BioRad iQ™ SYBR Green supermix (CAT#170-8882) with the following conditions: 95°C 3' then 40 cycles of 95°C 30", 60°C 30", and 72C 30”. Primer sequences for real time PCR were the following: PLAC1; PLAC1-PS1-sense, 5' - TTCACCAGTGAGCACAAAGC - 3' and PLAC1-PS1-antisense, 5' - CCAGTCTATGGAGCACAGCA - 3', PLAC1-PS2-sense 5' -GGGCAAATACAGACACAGCA -3' and PLAC1-PS2-antisense 5' -CCAGTCTATGGAGCACAGCA -3', GAPDH; GAPDH-sense, 5' - GCCAAGGTCATCCATGACAACTTTGG - 3' and GAPDH-antisense, 5' - GCCTGCTTCACCACCTTCTTGATGTC - 3', mATPsy6 ; mATGsy6-sense, 5’-GGGCGCAGTGATTATAGGCTT-3’ and mATPsy6-antisense, 5’-GGTGTAGGTGTGCCTTGTGGT-3’, RNASE T2; RNASE T2-sense, 5' -TGGGGATAAAACCATCCATC -3' and RNASE T2-antisense, 5'-AGCTGCTGGTCTTGCTTAGT-3'.
For analysis of EBV DNA in Mutu Clones, genomic DNA was isolated as describe at flemingtonlab.com. PCR analysis was carried out essentially as described above for RT-PCR analysis except that the following conditions were used: 95°C 3' then 40 cycles of 95°C 30", 55°C 30", and 72C 30”. The following primers were used to amplify different parts of the EBV genome: BamHI R primers (Rp); R-left, 5'-TAGTTAATGCCCCAGCCAGA-3', R-right, 5'-CTTTAAAAAGGCCGGCTGAC-3', BamHI M primers (BMRF1p) ; M-right, 5'-ACCTACATGACTAGCATCAAGCAA-3', and M-left, 5'-GGCCTCCATAGTTTACAGACAGAA-3', BamHI Q primers (Qp); Q-left, 5'-AAATTGGGTGACCACTGAGG-3' and Q-right, 5'-CATACACCGTGCGAAAAGAA-3', BamHI K primers (EBNA1); K-left, 5'-AAGGAGGGTGGTTTGGAAAG-3' and K-right, 5'-TGGAATAGCAAGGCCAATTCC-3'.
To generate dominant negative forms of EBNA1, full length EBNA1 was first excised from the plasmid, pCEP4, with ClaI and SacII. Since the SacII digestion cleaves the last few carboxy-terminal amino acids from the EBNA1 reading frame, two adaptors were synthesized which together encode the terminal amino acids from EBNA1 plus a BglII overhang (Adaptor 1-sense; 5’-GGAGGGTGATGACGGAGATGACGGAGATGAA-3’, Adaptor 1-antisense; 5’-CATCACCTCCTTCATCTCCGTCATCTCCGTCATCACCCTCCGC-3’, Adaptor 2-sense; 5’-GGAGGTGATGGAGATGAGGGTGAGGAAGGGCAGGAGTGA-3’, Adaptor 2-antisense; 5’-GATCTCACTCCTGCCCTTCCTCACCCTCATCTC-3’). The ClaI/SacII EBNA1 fragment was ligated to EcoRI and BglII cut pMSCV-neo vector in the presence of Adaptor 1 plus Adaptor 2, plus an EcoRI/ClaI adaptor (from eZclone Systems). Ligations were carried out in the presence of 1 ul T4 polynucleotide Kinase to phosphorylate adaptors during ligation reaction. This construction led to the generation of pMSCV-neo-EBNA1wt. pMSCV-neo-E1dn was generated from this plasmid by digesting pMSCV-neo-EBNA1wt with EcoRI and ApaI to excise the amino terminal EBNA1 sequences. The resulting vector plus carboxyl terminal EBNA1 sequences were then ligated to three adaptors which when linked together contain a translation initiation sequence, an HA epitope tag, the SV40 nuclear localization signal plus an EcoRI overhang at the 5’ end and an ApaI overhang at the 3’ end (DN adaptor 1-sense; 5’-AATTCTGCTGAAGATGATGGCCTATCCTTATG-3’, DN adaptor 1-antisense; 5’-CATCATCTTCAGCAG-3’, DN adaptor 2-sense; 5’-ATGTGCCTGACTATGCCGCCCCAAAGAAA-3’, DN adaptor 2-antisense; 5’-CATAGTCAGGCACATCATAAGGATAGGC-3’ , DN adaptor 3-sense; 5’-AAGCGAAAGGTGGCCGGCC-‘3, DN adaptor 3-antisense; 5’-GGCCACCTTTCGCTTTTTCTTTGGGGCGG-3’). Ligations were carried out in a standard ligation reaction except that 1 ul of T4 polynucleotide kinase was added to phosphorylate adaptors during ligation reaction. This strategy generated pMSCV-neo-EBNA1dn. pMSCV-puro-EBNA1dn was derived from this plasmid by simply excising the HA/nls/EBNA1dn cassette from pMSCV-neo-EBNA1dn and transferring to pMSCV-puro using appropriate standard cloning adaptors. All constructs were verified by sequence analysis.
We would like to thank Erik Flemington for input and for providing reagents. We would also like to thank Ratna Vadlamudi, Frank Jones and Matt Burrow for cell lines and for helpful suggestions. Thanks to Brian Schaefer for excellent technical advice and reagents. We thank Dr. Michael Fant for carefully reading our manuscript and for giving valuable modifications. We thank Dr. Joseph A. Lasky for helping publication. Acknowledge funding supports from the NIH T32 grant and Ladies Leukemia League.
- Cocchia M, Huber R, Pantano S, Chen EY, Ma P, Forabosco A, Ko MS, Schlessinger D: PLAC1, an Xq26 gene with placenta-specific expression. Genomics 2000, 68: 305-312.PubMedView Article
- Fant M, Weisoly DL, Cocchia M, Huber R, Khan S, Lunt T, Schlessinger D: PLAC1, a trophoblast-specific gene, is expressed throughout pregnancy in the human placenta and modulated by keratinocyte growth factor. Mol Reprod Dev 2002, 63: 430-436.PubMedView Article
- Galaviz-Hernandez C, Stagg C, de Ridder G, Tanaka TS, Ko MS, Schlessinger D, Nagaraja R: Plac8 and Plac9, novel placental-enriched genes identified through microarray analysis. Gene 2003, 309: 81-89.PubMedView Article
- Hemberger M, Himmelbauer H, Ruschmann J, Zeitz C, Fundele R: cDNA subtraction cloning reveals novel genes whose temporal and spatial expression indicates association with trophoblast invasion. Dev Biol 2000, 222: 158-169.PubMedView Article
- Massabbal E, Parveen S, Weisoly DL, Nelson DM, Smith SD, Fant M: PLAC1 expression increases during trophoblast differentiation: evidence for regulatory interactions with the fibroblast growth factor-7 (FGF-7) axis. Mol Reprod Dev 2005, 71: 299-304.PubMedView Article
- Kaufmann P, Mayhew TM, Charnock-Jones DS: Aspects of human fetoplacental vasculogenesis and angiogenesis. II. Change During Normal Pregnancy Placenta 2004, 25: 114-126.
- Zygmunt M, Herr F, Munstedt K, Lang U, Liang OD: Angiogenesis and vasculogenesis in pregnancy. Eur J Obstet Gynecol Reprod Biol 2003,110(Suppl 1):S10-S18.PubMedView Article
- Farina A, Rizzo N, Concu M, Banzola I, Sekizawa A, Grotti S, Carinci P: Lower maternal PLAC1 mRNA in pregnancies complicated with vaginal bleeding (threatened abortion <20 weeks) and a surviving fetus. Clin Chem 2005, 51: 224-227.PubMedView Article
- Jackman SM, Kong X, Fant ME: Plac1 (placenta-specific 1) is essential for normal placental and embryonic development. Mol Reprod Dev 2012, 79: 564-572.PubMedPubMed CentralView Article
- Scanlan MJ, Gure AO, Jungbluth AA, Old LJ, Chen YT: Cancer/testis antigens: an expanding family of targets for cancer immunotherapy. Immunol Rev 2002, 188: 22-32.PubMedView Article
- Koslowski M, Sahin U, Mitnacht-Kraus R, Seitz G, Huber C, Tureci O: A placenta-specific gene ectopically activated in many human cancers is essentially involved in malignant cell processes. Cancer Res 2007, 67: 9528-9534.PubMedView Article
- Kieff E: Epstein-Barr virus and its replication. 3rd edition. Philadelphia: Lippincott-Raven Press; 1996.
- Thompson MP, Kurzrock R: Epstein-Barr virus and cancer. Clin Cancer Res 2004, 10: 803-821.PubMedView Article
- Jacobson LP, Yamashita TE, Detels R, Margolick JB, Chmiel JS, Kingsley LA, Melnlck S, Muñoz A: Impact of potent antiretroviral therapy on the incidence of Kaposi's sarcoma and non-Hodgkin's lymphomas among HIV-1-infected individuals. Multicenter AIDS Cohort Study. J Acquir Immune Defic Syndr 1999,21(Suppl 1):S34-S41.PubMed
- Ledergerber B, Telenti A, Egger M: Risk of HIV related Kaposi's sarcoma and non-Hodgkin's lymphoma with potent antiretroviral therapy: prospective cohort study. Swiss HIV Cohort Study Bmj 1999, 319: 23-24.PubMed
- Lee MA, Diamond ME, Yates JL: Genetic evidence that EBNA-1 is needed for efficient, stable latent infection by Epstein-Barr virus. J Virol 1999, 73: 2974-2982.PubMedPubMed Central
- Young LS, Dawson CW, Eliopoulos AG: The expression and function of Epstein-Barr virus encoded latent genes. Mol Pathol 2000, 53: 238-247.PubMedPubMed CentralView Article
- Chodosh J, Holder V, Gan Y, Belgaumi A, Sample J, Sixbey J: Eradication of latent Epstein-Barr virus by hydroxyurea alters the growth-tranformed cell phenotype. J Invect Dis 1998, 177: 1194-1201.View Article
- Komano J, Sugiura M, Takada K: Epstein-Barr virus contributes to the malignant phenotype and to apoptosis resistance in Burkitt's lymphoma cell line Akata. J Virol 1998, 72: 9150-9156.PubMedPubMed Central
- Shimizu N, Tanabe-Tochikura A, Kuroiwa Y, Takada K: Isolation of Epstein-Barr virus (EBV)-negative cell clones from the EBV-positive Burkitt's lymphoma (BL) line Akata: malignant phenotypes of BL cells are dependent on EBV. J Virol 1994, 68: 6069-6073.PubMedPubMed Central
- Zhang RD, Fidler IJ, Price JE: Relative malignant potential of human breast carcinoma cell lines established from pleural effusions and a brain metastasis. Invasion Metastasis 1991, 11: 204-215.PubMed
- Janssens N, Janicot M, Perera T, Bakker A: Housekeeping genes as internal standards in cancer research. Mol Diagn 2004, 8: 107-113.PubMedView Article
- Lu H, Zhang Y, Roberts DD, Osborne CK, Templeton NS: Enhanced gene expression in breast cancer cells in vitro and tumors in vivo. Mol Ther 2002, 6: 783-792.PubMedView Article
- Revillion F, Pawlowski V, Hornez L, Peyrat JP: Glyceraldehyde-3-phosphate dehydrogenase gene expression in human breast cancer. Eur J Cancer 2000, 36: 1038-1042.PubMedView Article
- Murphy SP, Choi JC, Holtz R: Regulation of major histocompatibility complex class II gene expression in trophoblast cells. Reprod Biol Endocrinol 2004, 2: 52.PubMedPubMed CentralView Article
- Gilboa E: How tumors escape immune destruction and what we can do about it. Cancer Immunol Immunother 1999, 48: 382-385.PubMedView Article
- Curiel TJ, Coukos G, Zou L, Alvarez X, Cheng P, Mottram P, Evdemon-Hogan M, Conejo-Garcia JR, Zhang L, Burow M, Zhu Y, Wei S, Kryczek I, Daniel B, Gordon A, Myers L, Lackner A, Disis ML, Knutson KL, Chen L, Zou W: Specific recruitment of regulatory T cells in ovarian carcinoma fosters immune privilege and predicts reduced survival. Nat Med 2004, 10: 942-949.PubMedView Article
- Bulla R, Fischetti F, Bossi F, Tedesco F: Feto-maternal immune interaction at the placental level. Lupus 2004, 13: 625-629.PubMedView Article
- Acevedo HF, Tong JY, Hartsock RJ: Human chorionic gonadotropin-beta subunit gene expression in cultured human fetal and cancer cells of different types and origins. Cancer 1995, 76: 1467-1475.PubMedView Article
- Goshen R, Ariel I, Shuster S, Hochberg A, Vlodavsky I, de Groot N, Ben-Rafael Z, Stern R: Hyaluronan, CD44 and its variant exons in human trophoblast invasion and placental angiogenesis. Mol Hum Reprod 1996, 2: 685-691.PubMedView Article
- Devergne O, Birkenbach M, Kieff E: Epstein-Barr virus-induced gene 3 and the p35 subunit of interleukin 12 form a novel heterodimeric hematopoietin. Proc Natl Acad Sci U S A 1997, 94: 12041-12046.PubMedPubMed CentralView Article
- Devergne O, Coulomb-L'Hermine A, Capel F, Moussa M, Capron F: Expression of Epstein-Barr virus-induced gene 3, an interleukin-12 p40-related molecule, throughout human pregnancy: involvement of syncytiotrophoblasts and extravillous trophoblasts. Am J Pathol 2001, 159: 1763-1776.PubMedPubMed CentralView Article
- Van Krieken JH, Kluin PM: The association of c-myc rearrangements with specific types of human non-Hodgkin's lymphomas. Leuk Lymphoma 1992, 7: 371-376.PubMedView Article
- Deng Z, Chen CJ, Chamberlin M, Lu F, Blobel GA, Speicher D, Cirillo LA, Zaret KS, Lieberman PM: The CBP bromodomain and nucleosome targeting are required for Zta-directed nucleosome acetylation and transcription activation. Mol Cell Biol 2003, 23: 2633-2644.PubMedPubMed CentralView Article
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