Systemic distribution of different low pathogenic avian influenza (LPAI) viruses in chicken
© Post et al.; licensee BioMed Central Ltd. 2013
Received: 26 June 2012
Accepted: 15 January 2013
Published: 17 January 2013
Since we were able to isolate viable virus from brain and lung of H7N1 low pathogenic avian influenza virus (LPAIV) infected chickens, we here examined the distribution of different LPAIV strains in chickens by measuring the viral AI RNA load in multiple organs. Subtypes of H5 (H5N1, H5N2), H7 (H7N1, H7N7) and H9 (H9N2), of chicken (H5N2, H7N1, H7N7, H9N2), or mallard (H5N1) origin were tested. The actual presence of viable virus was evaluated with virus isolation in organs of H7N7 inoculated chickens.
Viral RNA was found by PCR in lung, brain, intestine, peripheral blood mononuclear cells, heart, liver, kidney and spleen from chickens infected with chicken isolated LPAIV H5N2, H7N1, H7N7 or H9N2. H7N7 virus could be isolated from lung, ileum, heart, liver, kidney and spleen, but not from brain, which was in agreement with the data from the PCR. Infection with mallard isolated H5N1 LPAIV resulted in viral RNA detection in lung and peripheral blood mononuclear cells only.
We speculate that chicken isolated LPAI viruses are spreading systemically in chicken, independently of the strain.
KeywordsLow pathogenic avian Influenza Chickens Systemic distribution
Avian influenza (AI) A is a highly heterogeneous group of viruses with varying pathogenicity in different species. Influenza virus subtypes have gained sufficient adaptive molecular changes to become established in domestic poultry and cause mild to severe disease [1–3]. AI is classified by the world organization for animal health into two pathotypes, low pathogenicity avian influenza viruses (LPAIV) and high pathogenicity avian influenza viruses (HPAIV), based on their virulence in chickens. Pandemic influenza outbreaks of HPAIV in poultry pose a significant threat to public health as highlighted by the emergence of H5N1 HPAIV . In general, the HPAIV emerged from a H5 or H7 LPAIV subtype that was circulating in chickens or turkeys . LPAIV haemagglutinin (HA) lacks the polybasic cleavage site that characterizes most HPAIV. The monobasic cleavage site of LPAIV favors trypsin-like proteases, which are thought to be secreted only by cells of the respiratory and intestinal tract. However, some chicken-isolated LPAIV strains have been isolated from a limited number of other tissues including the pancreas, kidneys and oviduct of intranasally (i.n.) or intratracheally (i.t.) inoculated chickens . In addition, we were able to isolate viable virus from brain of H7N1 LPAIV inoculated chickens . With the introduction of PCR several reports indicate systemic distribution of mRNA of LPAIV strains like H7N1 and H9N2 [6–8]. In order to investigate whether systemic distribution of LPAIV was restricted to some individually reported strains, we tested the systemic distribution of four LPAIV chicken (C-LPAIV) and one mallard (M-LPAIV) isolate, using PCR in chicken.
H7N1 LPAIV (A/Chicken/Italy/1067/99) was a gift from Dr. Ilaria Capua (Istituto Zooprofilattico Sperimentaledelle Venezie, Italy). H5N1 LPAIV (A/Mallard/Italy/3401/05), H5N2 LPAIV (A/Chicken/Pennsylvania/21525/83) and H7N7 LPAIV (A/Chicken/Netherlands/06022003/06) were kindly provided by Dr. Guus Koch (Department of Virology, Central Veterinary Institute of Wageningen UR, The Netherlands). H9N2 (A/Chicken/Saudi Arabia/SP02525/3AAV/2000) was obtained from the Animal Health Service (Deventer, The Netherlands). The viruses were propagated and titrated in the allantoic cavities of 10-day-old SPF chicken eggs to prepare stock virus. For animal experiments, the viruses were diluted in sterile PBS to 106 EID50 per ml immediately prior to use. Non-vaccinated AI-free chickens were randomly divided over four (experiment 1) or 2 (experiment 2) groups and housed in floor cages for 3 weeks prior to inoculation. Food and water were provided ad libitum. In compliance with Dutch law, all experiments were approved by the Animal Experimental committee of the institutions, in accordance with the Dutch regulations on experimental animals. For the first experiment one-day-old Lohmann Brown layer chickens were obtained from a commercial breeder (Pronk’s Broederij, Meppel, The Netherlands). Chickens were inoculated with 0.2 ml (2×105 EID50) of the LPAIV strains H7N1, H7N7 or H5N1 equally divided between the i.n. and i.t. route. Control chickens were inoculated with 0.2 ml PBS. Six chickens from each group were sacrificed at 2 and 4 days post infection (d.p.i.). The body weight of the chickens was established daily and from all sacrificed chickens gross pathology of the organs was studied. Lung, brain, ileum, blood, liver, kidney, heart and spleen were collected for RNA extraction, snap-frozen in liquid nitrogen and stored at −80°C until use. A piece of about 0.5×0.5 cm tissue was used which correspond to approximately 200 mg. peripheral blood mononuclear cells (PBMC) were isolated from blood by ficol-paque (Amersham Biosciences, Uppsala, Sweden). For the second experiment the chickens were obtained from a commercial breeder and inoculated as in exp. 1 with LPAIV H5N2 or H9N2. Five chickens from each group were sacrificed at 2 and 4 d.p.i. The body weight of the H5N2 LPAIV inoculated chickens was established at 0, 1, 2 and 4 d.p.i.
For RNA isolation, organs were stored in trizol (Invitrogen, Breda, The Netherlands) at −80°C until use. Both methods of preservation were tested and give equal RNA values. RNA isolation of the samples of both experiments was performed using the phenol/chloroform method as previously described . H7N1, H7N7, H5N1 and H5N2 were analyzed using the quantitative PCR (qPCR) . For H9N2 cDNA was generated from 500 ng RNA with reverse transcription using an iScript cDNA Synthesis kit (Biorad, Veenendaal, The Netherlands). H9N2 viral cDNA was amplified with specific primers and conditions as described . RNA samples of day 2 p.i. of H9N2 infected chickens were tested using an one and two step PCR with the primers described. Comparable Ct values for AI load were obtained. Therefore both PCR methods can be used next to each other. As a control, tissue samples of the uninfected chickens were included in each run of the qPCR. Standardization of the PCR was done by equalizing the amount of input RNA with 200 ng. The quality and integrity of the RNA samples was analyzed using the Agilent Bioanalyzer (lab on chip, Agilent). The data were expressed as Ct-45 values, which means that increased values in the figures indicate increased amount of viral RNA and when Ct-45 is equal to zero no viral RNA was detected. Because no Ct values were found for any organs of control chickens tested during a 45 cycles run in these particular PCRs, we considered Ct values ≤ 45 as positive . H7N7 inoculated chickens from the first experiment were used as an example to confirm the presence of progeny virus in tissues where viral RNA was detected by PCR . Briefly, the supernatant of homogenized and clarified organs of six H7N7 inoculated chickens (4 d.p.i.) was divided over five embryonated eggs. The eggs were screened daily and incubated for 7 days. After 7 days or earlier after embryo mortality the allantoic fluid was harvested. The presence of H7N7 virus in the allantoic fluid was tested with the HA-test and sequencing. Differences between the Ct values of the strains were analyzed for statistical significance by the Mann–Whitney U test. For this purpose the Ct value of samples with no detectable virus was set at 45. Differences in weight was analyzed for statistical significance by the Student’s T-test. P ≤ 0.05 was considered significantly different.
Although viral RNA was detected in most lungs and spleens after inoculation with C-LPAIV strains, differences were found between the C-LPAIV strains. After a H5N2 C-LPAIV infection all chickens become positive for viral RNA and almost all evaluated organs of these chickens were infected (Figures 1 and 2). At 2 d.p.i. a significant higher viral load was found in PBMC (compared to H7N1, H7N7 (p ≤ 0.01) and H9N2 (p ≤ 0.05)), heart (compared to H7N1 and H9N2 (p ≤ 0.01)) and kidney (compared to H9N2 (p ≤ 0.01)). At 4 d.p.i. a significant higher viral load was found in brain (compared to H7N1, H7N7 (p ≤ 0.01) and H9N2 (p ≤ 0.05)) and PBMC (compared to H7N1 (p ≤ 0.01) and H7N7 (p ≤ 0.05)). A typically high viral RNA load was found in the kidney of H7N1 inoculated chickens at 4 d.p.i. (significant differences compared to H7N7, H9N2 (p ≤ 0.01) and H5N2 (p ≤ 0.05)), in the ileum of H7N7 inoculated chickens at 2 d.p.i. (significant differences compared to H7N1 (p ≤ 0.05)) and in the PBMC of H9N2 inoculated chickens (significant differences compared to H7N1 and H7N7 (p ≤ 0.05)). Figures 1 and 2 show that not all chickens were positive in brain (H7N1 and H7N7), heart (H7N1 and H9N2) and kidney (H9N2). The cause of the differences in distribution between the C-LPAIV strains has not been studied here, but might for instance be related to receptor affinity [9, 10] or replication efficiency as suggested for differences between HPAIV .
Detection of virus in organs of H7N7 LPAIV inoculated chickens by virus isolation and HA
Virus present (pos. chickens/total tested)
While there is sufficient literature on C-LPAIV, reports studying M- LPAIV infections are scarce. Ladman et al.  examined the upper respiratory and the intestinal tract of turkeys and chickens after inoculation of different LPAI viruses, including the mallard isolates H5N1 and H7N3. H5N1 LPAIV could only be isolated from the upper respiratory tract of turkeys, while H7N3 LPAIV was isolated from the upper respiratory and the intestinal tract of both turkeys and chickens. The fact that H5N1 LPAIV was detected in turkeys and not in chicken might be explained by a difference in susceptibility for AI between turkeys and chickens . Although the tissue tropism of the mallard isolates LPAIV H5N1 and H7N3 might differ in chicken, we speculate that differences between H5N1 M- LPAIV and the C- LPAIV subtypes that were tested in our study were related to differences in adaptation to the host. Starting from the primary infected organs, systemic distribution is among others facilitated by lymphocytes [18, 19]. In chickens with viral RNA of H5N1 M-LPAIV in the lung (Figure 2) we also found viral RNA in PBMC at 4 d.p.i., confirming the relation between the level of viral RNA in the primary infected lung and possibilities for systemic distribution via blood cells. In the primary infected lung, where the necessary proteases for LPAIV replication are available, H5N1 M-LPAIV RNA could not be measured in 4 out of 6 chickens on day 2 and 4 (Figures 1 and 2). When the experiment was repeated the results were largely identical (data not shown) and the absence of viral RNA in the lung of H5N1 M-LPAIV infected chickens was therefore probably not related to the experimental infection method. The absence of virus in the lung (and other organs) of H5N1 M-LPAIV infected chickens might be related to impaired replication opportunities of the virus in the non-mallard host. Why some H5N1 M-LPAIV infected chickens did have a high RNA load in the lung is unclear.
In summary, RNA of a panel of LPAI viruses isolated from chickens can be detected in multiple organs of chickens. Viral RNA might indicate viable virus in organs beyond the respiratory tract as was shown for H7N1 and H7N7 LPAIV. Differences in systemic distribution between the LPAIV strains isolated from chickens exist but larger differences are found between the chicken isolated strains and the mallard isolated strain. These differences are likely to be dependent on host preference and might eventually be related to replication efficiency of the virus in different hosts.
Low pathogenic avian influenza virus
Chicken isolated low pathogenic avian influenza virus strain
Mallard isolated low pathogenic avian influenza virus strain
Days post infection
Financially supported by EU 6th framework Flupath (grant 044220) and program “Impulse Veterinary avian influenza research in the Netherlands” Dutch Ministry of Agriculture, Nature and Food Quality.
- Alexander DJ: An overview of the epidemiology of avian influenza. Vaccine 2007, 25: 5637-5644. 10.1016/j.vaccine.2006.10.051PubMedView ArticleGoogle Scholar
- Iqbal M, Yaqub T, Reddy K, McCauley JW: Novel genotypes of H9N2 influenza A viruses isolated from poultry in Pakistan containing NS genes similar to highly pathogenic H7N3 and H5N1 viruses. PLoS One 2009, 4: e5788. 10.1371/journal.pone.0005788PubMedPubMed CentralView ArticleGoogle Scholar
- Kwon J, Lee H, Lee D, Lee Y, Mo I, Nahm S, Kim M, Lee J, Park S, Choi I, Song C: Immune responses and pathogenesis in immunocompromised chickens in response to infection with the H9N2 low pathogenic avian influenza virus. Virus Res 2008, 133: 187-194. 10.1016/j.virusres.2007.12.019PubMedView ArticleGoogle Scholar
- Ferguson NM, Fraser C, Donnelly CA, Ghani AC, Anderson RM: Public health risk from the avian H5N1 influenza epidemic. Science 2004, 304: 968-969. 10.1126/science.1096898PubMedView ArticleGoogle Scholar
- Spickler AR, Trampel DW, Roth JA: The onset of virus shedding and clinical signs in chickens infected with high-pathogenicity and low pathogenicity avian influenza viruses. Avian Pathol 2008, 37: 555-577. 10.1080/03079450802499118PubMedView ArticleGoogle Scholar
- Post J, Burt DW, Cornelissen JBWJ, Broks V, Van Zoelen D, Peeters B, Rebel JMJ: Systemic distribution and host responses in brain and intestine of chickens infected with low pathogenic or high pathogenic avian influenza virus. Virol J 2012, 6: 9-61.Google Scholar
- Mosleh N, Dadras H, Mohammadi A: Molecular quantitation of H9N2 avian influenza virus in various organs of broiler chickens using TaqMan real time PCR. J Mol Genet Med 2009, 3: 152-157.PubMedPubMed CentralView ArticleGoogle Scholar
- Toffan A, Beato MS, De Nardi R, Bertoli E, Salviato A: Conventional inactivated bivalent H5/H7 vaccine prevents viral localization in muscles of turkeys infected experimentally with low pathogenic avian influenza and highly pathogenic avian influenza isolates. Avian Pathol 2008, 37: 407-412. 10.1080/03079450802061124PubMedPubMed CentralView ArticleGoogle Scholar
- Gambaryan A, Tuzikov A, Pazynina G, Bovin N, Balish A, Klimov A: Evolution of the receptor binding phenotype of influenza A (H5) viruses. Virology 2006, 344: 432-438. 10.1016/j.virol.2005.08.035PubMedView ArticleGoogle Scholar
- Mundt E, Gay L, Jones L, Saavedra G, Tompkins SM, Tripp RA: Replication and pathogenesis associated with H5N1, H5N2, and H5N3 low-pathogenic avian influenza virus infection in chickens and ducks. Arch Virol 2009, 154: 1241-1248. 10.1007/s00705-009-0437-2PubMedView ArticleGoogle Scholar
- Bogs J, Veits J, Gohrbandt S, Hundt J, Stech O, Breithaupt A, Teifke JP, Mettenleiter TC, Stech J: Highly pathogenic H5N1 influenza viruses carry virulence determinants beyond the polybasic hemagglutinin cleavage site. PLoS One 2010, 5: e11826. 10.1371/journal.pone.0011826PubMedPubMed CentralView ArticleGoogle Scholar
- Gharaibeh S: Pathogenicity of an avian influenza virus serotype H9N2 in chickens. Avian Dis 2008, 52: 106-110. 10.1637/8108-090907-RegPubMedView ArticleGoogle Scholar
- Li C, Yu K, Tian G, Yu D, Liu L, Jing B, Ping J, Chen H: Evolution of H9N2 influenza viruses from domestic poultry in mainland China. Virology 2005, 340: 70-83. 10.1016/j.virol.2005.06.025PubMedView ArticleGoogle Scholar
- Wan H, Sorrel EM, Song H, Hossain MJ, Ramirez-Nieto G, Monne I, Stevens J, Cattoli G, Capua I, Chen L, Donis RO, Busch J, Paulson JC, Brockwell C, Webby R, Blanco J, Al-Natour MQ, Perez DR: Replication and transmission of H9N2 influenza viruses in ferrets: Evaluation of pandemic potential. PLoS One 2008, 3: e2923. 10.1371/journal.pone.0002923PubMedPubMed CentralView ArticleGoogle Scholar
- Monne I, Ormelli S, Salviato A, De Battisti C, Bettini F, Salomoni A, Drago A, Zecchin B, Capua I, Cattoli G: Development and validation of a one-step real-time PCR assay for simultaneous detection of subtype H5, H7, and H9 avian influenza viruses. J Clin Microbiol 2008, 46: 1769-1773. 10.1128/JCM.02204-07PubMedPubMed CentralView ArticleGoogle Scholar
- Ladman BS, Driscoll CP, Pope CR, Slemons RD, Gelb J Jr: Potential of low pathogenicity avian influenza viruses of wild bird origin to establish experimental infections in turkeys and chicken. Avian Dis 2010, 54: 1091-1094. 10.1637/9228-010410-ResNote.1PubMedView ArticleGoogle Scholar
- Spackman E, Gelb J Jr, Preskenis LA, Ladman BS, Pope CR, Pantin-Jackwood MJ, McKinley ET: The pathogenesis of low pathogenicity H7 avian influenza viruses in chickens, ducks and turkeys. Virol J 2010, 7: 331. 10.1186/1743-422X-7-331PubMedPubMed CentralView ArticleGoogle Scholar
- Van Campen H, Easterday BC, Hinshaw VS: Destruction of lymphocytes by a virulent avian influenza A virus. J Gen Virol 1989, 70: 467-472. 10.1099/0022-1317-70-2-467PubMedView ArticleGoogle Scholar
- Gabriel G, Klingel K, Planz O, Bier K, Herwig A, Sauter M, Klenk HD: Spread of infection and lymphocyte depletion in mice depends on polymerase of influenza virus. Am J Pathol 2009, 175: 1178-1186. 10.2353/ajpath.2009.090339PubMedPubMed CentralView ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.