A monoclonal antibody against lymphocyte function-associated antigen-1 decreases HIV-1 replication by inducing the secretion of an antiviral soluble factor
© Rychert et al.; licensee BioMed Central Ltd. 2013
Received: 13 December 2012
Accepted: 12 April 2013
Published: 18 April 2013
Lymphocyte Function-Associated Antigen-1 (LFA-1) likely plays a role in the pathogenesis of against HIV-1 and is known to facilitate cell-to-cell transmission of the virus. A monoclonal antibody specific for LFA-1 (Cytolin®) was evaluated as a potential therapeutic in pilot studies performed in the mid-1990s. These uncontrolled human studies suggested that administration of this anti-LFA-1 antibody to HIV-1 infected individuals could provide a modest benefit by decreasing circulating HIV-1 RNA and increasing CD4+ T cell counts. At the time, it was proposed that when bound to cytolytic T cells, the antibody inhibited lysis of activated CD4+ T cells. Given the renewed interest in monoclonal antibody therapy for HIV-1 infected individuals, we investigated possible mechanisms of action of this antibody in vitro.
To assess whether this anti-LFA-1 antibody binds to HIV-1, a virus capture assay was performed. Binding of the antibody to cells was assessed using flow cytometry. Inhibition of HIV-1 replication was determined in culture by measuring the amount of p24 produced by ELISA. After co-culture of the antibody with peripheral blood mononuclear cells, supernatants were assayed for cytokines and chemokines using various immunoassays.
Our experiments demonstrate that anti-LFA-1 antibody binds to CCR5 and CXCR4 utilizing strains of HIV-1. It also binds to CD8+ T cells and dendritic cells. When bound to virus prior to infection, there is no decrease in HIV-1 replication, suggesting it does not directly inhibit viral replication via virus binding. When bound to cells, it does not inhibit lysis of CD4+ T cells, as was originally hypothesized. Binding to cells does appear to induce the production of a soluble factor that inhibits HIV-1 replication. We determined that this soluble factor was not any of the cytokines or chemokines with known anti-HIV-1 activity. Further, the antibody does not appear to induce any common immune modulating cytokines or chemokines.
These results suggest that one possible mechanism of action of this anti-LFA-1 antibody is to inhibit HIV-1 replication via the production of a soluble antiviral factor that is induced upon binding to cells.
KeywordsHIV-1 LFA-1 Monoclonal antibody therapy
Lymphocyte funtion-associated antigen
Human immunodeficiency virus
Enzyme linked immunosorbent assay
Cytotoxic T lympocyte
Peripheral blood mononuclear cells
Carboxyfluorescein succinimidyl ester
Phosphate buffered saline
Macrophage inflammatory protein
Regulated and normal T cell expressed and secreted
Human neutrophil protein
Epidermal growth factor
Stromal cell-derived factor 1
Macrophage derived chemokines
Leukemia inhibitory factor
Monocyte chemoattractant protein
Transforming growth factor beta
CD8 antiviral factor.
Lymphocyte Function-Associated Antigen-1 (LFA-1, CD11a/CD18) is a member of the integrin family of adhesion molecules. It is expressed on immune cells and plays a role in leukocyte trafficking, antigen presentation, cellular activation, and adhesion of Cytotoxic T lymphocytes (CTL) to their targets. In addition to its role in the immune response, LFA-1 and its ligands are incorporated into the viral envelope as HIV-1 buds from the cell surface . These proteins facilitate viral synapse formation and promote cell-to-cell transmission of the virus .
Cytolin® is a murine anti-human monoclonal antibody that binds to LFA-1 (hereafter referred to as LFA-1 MAb). It recognizes an epitope within CD11a known as S6F1. This epitope is preferentially expressed on CD8+ T cells and can be used to distinguish killer effector cells from suppressor effector cells . In HIV-1 infected individuals, CD8+ T cells play a central role in controlling viral replication by lysing infected cells. It has been shown that the frequency of CD8+ T cells expressing the S6F1 epitope is higher in HIV-1 infected individuals compared to uninfected controls [4, 5]. The S6F1+ cell subset is not expanded in individuals with Epstein Barr Virus (EBV) infection , suggesting this epitope may play a unique role in the pathogenesis of HIV-1 infection and is not merely the result of expansion of this cell subset due to chronic viral infection.
It has been hypothesized that LFA-1 MAb could be used therapeutically to alter the course of HIV-1 infection. In several small clinical studies [7, 8] a reduction in HIV-1 RNA (range 0.2-1 log10 copies/ml) and a modest increase in CD4 T cell count (range 70–200 cells/mm3) was observed when the antibody was administered to HIV-1 infected individuals. At the time, investigators hypothesized that LFA-1 MAb improved CD4 T cell counts by inhibiting the cytolytic effect of CD8+ T cells (CTL) on activated uninfected CD4+ T cells . This hypothesis was based on in vitro studies that suggested that CTL killing of activated uninfected CD4+ T cells contributes to CD4+ T cell depletion in HIV-1 infected individuals [9, 10]. This lytic activity was shown to be abrogated using an antibody specific to LFA-1 . Given the increased interest in monoclonal antibody therapy in persons with HIV-1 infection, we set out to determine possible mechanisms of action of LFA-1 MAb.
n = 25
Mean Age (years)
(n = 13)
(n = 12)
Mean HIV Plasma Viral Load at first visit copies/ml (range)
Mean Absolute CD4 Count at first visit cells/mm3 (range)
Mean Absolute CD8 Count at first visit cells/mm3 (range)
p < 0.0001
Inhibiting HIV-1 replication via virus binding
We then asked whether HIV-1 replication could be inhibited in the presence of LFA-1 MAb. The same viral stocks were incubated with increasing concentrations of LFA-1 MAb prior to infecting PHA activated CD8-depleted PBMC. These infection assays were performed in triplicate using cells from ten of the HIV-1 negative subjects. As shown in Figure 1B, there was a trend for a decrease in replication with higher concentrations of LFA-1 MAb for HIV-IIIB and to a lesser extent HIV-SF162. However, the mean concentration of p24 was not significantly different when virus was pre-incubated with LFA-1 MAb for any of the three viral isolates (p = 0.07 for IIIB, p = 0.55 for SF162, p = 0.56 for AC225, one way ANOVA). Although LFA-1 MAb is able to bind to CXCR4 and CCR5 utilizing strains of HIV-1, this does not result in the inhibition of viral replication.
Inhibiting HIV replication via cell binding
Identifying the antiviral soluble factor
In this study, we set out to determine the in vitro mechanisms of action of a monoclonal antibody that binds to Lymphocyte Function-Associated Antigen-1 (LFA-1). We found that LFA-1 MAb is able to bind HIV-1 virions and preferentially binds to CD8+ T cells and dendritic cells. When bound to virus, it does not inhibit HIV-1 replication in culture. When bound to cells, it does not inhibit CTL killing of activated uninfected CD4+ T cells, as was previously hypothesized; rather, it appears to induce the production of an as yet unidentified soluble factor that can inhibit HIV-1 replication.
Using a virus capture assay, we determined that LFA-1 MAb is able to bind to HIV-1. However, it does not appear to bind to CD4+ T cells. We know that LFA-1 takes on different conformations based on the activation state of a cell [16, 17]. It is possible, that under specific conditions, infected CD4+ T cells express LFA-1 in such a way that LFA-1 MAb could bind. Indeed, others have shown that HIV-1 gp120 can trigger the activated state of LFA-1 in a CD4 dependent manner . It has also been shown that binding of α4β7 by gp120 results in an altered conformation of LFA-1 on CD4+ T cells . It is therefore possible that HIV-1 infection itself results in changes in the conformation of LFA-1 on CD4+ T cells such that they express the S6F1 epitope to which LFA-1 MAb binds. Alternatively, LFA-1 MAb may only bind to the virions that replicated within dendritic cells, which contain LFA-1 on their surface.
Incubating PBMC with LFA-1 MAb for 24 hours resulted in the production of a soluble factor that inhibited HIV-1 replication. Given the cells that LFA-1 MAb binds and the short period of incubation, we hypothesized that this factor was most likely a chemokine or cytokine. However, we did not observe an increase in the concentration of any of the analytes we tested in supernatants from LFA-1 MAb exposed PBMC, as compared to control. It is possible that a combination of these factors may explain the anti-HIV-1 activity we observed or that the antiviral soluble factor may vary from one culture to another. This is difficult to assess given the small number of cultures tested. Another possibility is that residual LFA-1 Mab in the supernatant contributed to the inhibition we observed. However, we did not observe inhibition of viral replication when LFA-1 MAb is incubated with virus prior to infection. It is also possible that this soluble factor is the same anti-HIV-1 soluble factor, known as “CD8 Antiviral Factor” (CAF). CAF is known to be produced by CD8+ T cells ; however, it is typically produced after stimulation with anti-CD3 antibodies and its production is maximized after 5–9 days of culture. Therefore, it seems unlikely that the soluble factor is CAF. Further studies to identify the soluble factor are warranted. Biochemical experiments to determine it’s approximate size, it’s sensitivity to proteases, and it’s tolerance to changes in pH and temperature may yield important clues to its identity; however, ultimately it may be necessary to isolate it from the complex mixture of analytes in the cell culture supernatant and produce it in sufficient quantities to get a definitive identification.
In this study, we have shown that LFA-1 MAb may be able to inhibit HIV-1 replication as a result of binding to either CD8+ T cells or dendritic cells and inducing the production of an antiviral soluble factor. This is just one of the potential mechanisms by which this antibody could decrease HIV-1 replication in vivo. Other potential mechanisms include interfering with cell-to-cell transmission of virus or augmenting the HIV-1 specific immune response by altering leukocyte trafficking. Additional studies are needed to examine these mechanisms in more detail.
Our approach to identifying the antiviral soluble factor does give us some insight into the impact that LFA-1 MAb may have on the immune system. Despite binding to a signaling molecule that has the potential for altering an immune response, we did not identify any chemokine or cytokine that was produced as a consequence of the interaction between LFA-1 MAb and LFA-1. Although not shown here, we also did not observe an increase in proliferation or apoptosis in cultures containing LFA-1 MAb. Thus, this antibody appears to have a benign effect, in general, on immune cells. This could be advantageous if this antibody is further tested in human trials.
From this study we conclude that the mechanism of action of LFA-1 MAb is not the result of the antibody binding to virus, but rather a downstream effect of it binding to cells. Our data suggest that binding of LFA-1 MAb to CD8+ T cells or dendritic cells may result in the production of a yet-to-be-identified soluble antiviral factor.
Cytodyn Incorporated (Santa Fe, New Mexico) provided the anti-LFA-1 monoclonal antibody (Cytolin®). HIV-SF162 and HIV-IIIB were obtained from the NIH AIDS Reference and Reagent Program. These two viruses were chosen because they utilize either CCR5 or CXCR4 as a co-receptor for viral entry (SF162 and IIIB respectively). HIV-AC225 is a primary isolate from a recently infected individual. It is predicted to be a CCR5 utilizing isolate based on the sequence of the V3 region of its envelope. All three viruses were propagated on CD8-depleted PBMC from the same donor. Culture media was prepared using RPMI supplemented with Hepes, Penicillin/Streptomycin, L-glutamine, and 10% fetal calf serum.
Twenty-five subjects (13 HIV-1 negative and 12 HIV-1 positive) were enrolled in a blood draw only study. To be included in the study, HIV-1 infected subjects had to be in the asymptomatic stage of infection with a CD4 T cell count greater than 350 cells/mm3, an HIV-1 plasma RNA viral load less than 100,000 copies/ml, and not be on antiretroviral therapy. All subjects signed informed consent as approved by the Massachusetts General Hospital (MGH) Human Subjects Committee.
Peripheral blood samples were obtained by venipuncture into tubes containing Acid Citrate Dextrose (ACD). Plasma was separated from whole blood using centrifugation. Peripheral blood mononuclear cells (PBMC) were obtained by density gradient centrifugation (FICOLL, Sigma). HIV-1 serostatus was confirmed at the initial visit in all subjects. T cell subset analysis was performed at every visit for all subjects. HIV-1 RNA testing was performed at the initial visit for all subjects, and then only for HIV-1 infected subjects at subsequent visits. Assays were performed in the Clinical Laboratory at MGH.
To determine whether LFA-1 MAb could bind to HIV-1, a virus capture assay was performed similar to what has previously been described [21–23]. Briefly, ninety-six well plates (Nunc) were coated with 5ug/ml of the anti-LFA-1 antibody, PBS, or 1 ug/ml mixture of monoclonal antibodies specific to HIV-1 gp120, for 2 hours at room temperature. The gp120-specific antibody mixture contained three human monoclonal antibodies, 17b, A32, and EH21. These antibodies bind to discontinuous epitopes, are known to cross-react with envelope glycoproteins from multiple Clade B isolates, and likely bind to monomeric gp120 [24–27]. After blocking with PBS containing 4% whey, undiluted virus stocks were plated in triplicate, and incubated at room temperature for 2 hours. Wells were washed with PBS containing 0.05% Tween20, and harvested with 100ul PBS containing 0.5% TritonX 100. This supernatant was kept at 4°C overnight then diluted to 1:500 in PBS containing lysing solution. The presence of virus in these supernatants was determined using a p24 ELISA, as per the manufacturers instructions (HIV-1 p24 ELISA, Zeptometrix).
Isolation of cell subsets
PBMC were depleted of CD8+ cells by magnetic bead based separation using Dynabead CD8 (Invitrogen) as per the manufacturers instructions. This resulted in >95% depletion of CD8+ cells. CD4+ cells were isolated from PBMC by positive selection using paramagnetic beads coupled to anti-CD4 monoclonal antibodies (Dynabead CD4, Invitrogen). This isolation was performed according to the manufacturers instructions and resulted in a population of cells containing >95% CD4+ cells.
CTL lysis of activated CD4+ T cells
To determine whether LFA-1 MAb abrogated CTL lysis of activated uninfected CD4+ cells, we first obtained CD4+ target cells using magnetic positive enrichment, as described above. These cells were activated for three days at 37°C with 1.25 ug/ml PHA (phytohemagglutinin) in media containing 50U/ml IL-2. Autologous CD4+ cell depleted PBMC were used as effector cells. These cells were incubated with 10 ug/ml of LFA-1 MAb or PBS, as a control, on ice for 30 minutes then added without washing to the target cells. The activated CD4+ target cells were differentiated from effector cells via staining with 1uM CFSE. Effector and target cells were combined at a ratio of 25:1. After a 4 hour co-culture at 37°C, all cells were stained with 1 ug/ml 7AAD for 20 minutes at room temperature. The cells were then washed in PBS containing 2 ug/ml Actinomycin D and 1% fetal calf serum. Prior to acquisition, the cells were fixed in Actinomycin D containing buffer with 1% formaldehyde. The frequency of CFSE + 7AAD + cells (dead targets) was determined by flow cytometry on a BD LSRII. Single stained controls were used to set the gates. Percent cytotoxicity was calculated using the following formula: 100*(%sample lysis-%target cell only lysis)/(100-% target cell only lysis).
Identifying cells that bind LFA-1 MAb
To determine the cell subsets that bind to this anti-LFA-1 antibody, flow cytometry was performed. PBMC were either left inactivated or were activated for 1 hour at 37°C with 10 ng/ml of PMA (phorbol 12-myristate 13-acetate) and 5 ug/ml Ionomycin. The cells were then incubated with 24 ug/ml of LFA-1 MAb for 20 minutes at 4°C. This concentration of anti-LFA-1 was determined by titration on PBMC using flow cytometry. After washing in PBS containing 1% fetal calf serum, cells were stained with a FITC-labeled anti-mouse IgG for 20 minutes at 4°C. The various cell subsets were identified using fluorochrome-labeled antibodies to discriminate T cells, B cells, monocytes, and dendritic cells (CD3 PE, CD4 Q605, CD8 APC-Cy7, CD14 Pacific Blue, CD19 PE-Cy5, CD11c APC). The cells were washed and fixed in 1% formaldehyde prior to acquisition on a BD LSR-II. Gating was performed manually to identify CD3+ CD19- T cells, CD19 + CD3- B cells, and CD19-CD3- monocytes and dendritic cells. The T cell subset was further differentiated into CD4+ and CD8+ cells. Dendritic cells were defined as CD14- CD11c+, while monocytes were defined as CD14+ CD11c+. The percent of LFA-1 MAb + cells within each cell subset is reported.
Induction of soluble antiviral factors
To induce the production of an antiviral soluble factor, ninety-six well round bottom plates (BD Falcon) were coated with 1 ug/ml of LFA-1 MAb or PBS as a control and stored up to 1 week at 4°C. On the day of the assay, plates were warmed to room temperature and 2 × 105 PBMC were added to each well. After incubation at 37°C for 24 hours, the plates were centrifuged at 1700 rpm for 7 minutes to pellet the cells and the supernatant was removed and stored at −20°C prior to use in subsequent assays.
To test the direct effect of LFA-1 MAb on HIV-1 replication, the antibody was incubated with virus then a viral replication assay was performed. One hundred TCID50 of each virus stock (HIV-SF162, HIV-IIB, HIV-AC225) was incubated for 4 hours at 37°C with serial 10-fold dilutions of LFA-1 MAb. This mixture was then used to infect CD8-depleted PBMC from a single HIV-1 negative donor that had been activated for 3 days with 1.25 ug/ml of PHA in RPMI supplemented with 50U IL-2. Infected cells were maintained at 37°C for 7 days. The degree of viral replication was determined by measuring the concentration of p24 in the supernatant using an ELISA, as per the manufacturers instructions (HIV-1 p24 ELISA, Zeptometrix).
To test the effect of induced soluble factors on HIV-1 replication, a viral replication assay was performed in the presence of supernatant from PBMC treated with LFA-1 MAb or PBS, as described above. PHA activated CD8-depleted PBMC from a single HIV-1 negative donor were resuspended in supernatants diluted 1:2 with culture media, then infected with 10 TCID50 of HIV-SF162 and incubated at 37°C for 7 days. The concentration of p24 was then measured using an ELISA as per the manufacturers instructions (HIV-1 p24 ELISA, Zeptometrix).
Several assays were used in an attempt to determine the identity of the soluble factor. We started by testing for the presence of cytokines and chemokines that are known to have anti-HIV activity. The concentrations of MIP1-alpha, MIP1-beta, and RANTES were determined using a multiplex bead based ELISA system (Flowcytomix, Bender). Twenty-five microliters of supernatant from PBMC cultures incubated with either LFA-1 MAb or PBS (see above) were mixed with antibody-labeled beads, and biotin-conjugated secondary antibodies. After 2-hours of incubation, the beads were pelleted by centrifugation and incubated with PE-Streptavidin for 1 hour. The beads were then washed and resuspended in assay buffer containing 1% formaldehyde to ensure the samples were not infectious. Preliminary experiments verified that the use of formaldehyde did not interfere with the measurement of the analytes. The fluorescent signal from the beads was detected using a BD LSR-II flow cytometer. The resulting data was analyzed using the Flowcytomix software provided by the manufacturer. The concentration of each chemokine was determined by comparison to a standard curve generated from recombinant chemokines provided by the manufacturer.
An ELISA was performed to determine the concentration of alpha-defensins 1, 2 and 3 (Human HNP 1–3, Hycult Biotech) and Interferon-alpha (Human IFN-alpha pan ELISA kit, MAbtech). One hundred microliters of supernatant from PBMC cultures incubated with LFA-1 MAb or PBS were diluted 1:2 in dilution buffer and the assay was performed in duplicate according to each manufacturer’s instructions. The captured analytes were detected using a biotinylated secondary detection antibody followed by Streptavidin-peroxidase, and developed using a TMB substrate. Serial dilutions of the standards provided by each manufacturer were run in duplicate to create a standard curve from which the concentration of alpha defensins or IFN-alpha was calculated.
To broaden our search for the identity of the soluble factor, we utilized an antibody array (G2000 Human Cytokine Array, RayBioTech). This assay is similar to a standard sandwich ELISA, but it is performed on a glass slide and utilizes a fluorescent readout. This array can detect 174 different analytes including common cytokines, chemokines, and growth factors. A full list of the analytes can be found in Additional file 1. Supernatant from PBMC cultures incubated with either LFA-1 MAb or PBS (see above) was sent to RayBiotech for testing. Since the cell culture media we used in this assay contained serum, which includes many of the analytes being measured, a media only control was also tested. The mean signal intensity of LFA-1 MAb treated supernatants was compared to the mean signal intensity of PBS treated supernatants after subtracting out the signal intensity of the media control. A Wilcoxon sign rank test was used to determine whether the mean signal intensity was higher in LFA-1 MAb treated supernatants.
A follow-up experiment was performed to determine the concentration of Epidermal Growth Factor (EGF) in the LFA-1 MAb and PBS treated supernatants. In this quantitative ELISA, 100ul of supernatant was diluted 1:2 in dilution buffer and the assay was performed in duplicate as indicated by the manufacturer (EGF Human ELISA kit, MAbtech). Duplicate wells of serial dilutions the EGF standard were run in parallel with the samples to generate a standard curve from which the concentration of EGF was calculated.
The following reagent was obtained through the AIDS Research and Reference Reagent Program, Division of AIDS, NIAID, NIH: HIV-1 SF162 from Dr. Jay Levy and HTLV-IIIB/H9 from Dr. Robert Gallo.
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